ONEC_DROME
ID ONEC_DROME Reviewed; 1081 AA.
AC Q9NJB5; Q9V4E4;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 27-APR-2001, sequence version 2.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Homeobox protein onecut;
GN Name=onecut; ORFNames=CG1922;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Nguyen D.N.T., Lai Z.C.;
RT "The Drosophila onecut is a neural-specific transcriptional regulator.";
RL Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Transcriptional regulator. Binds and recognize ATTG sites.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Neural-specific.
CC -!- SIMILARITY: Belongs to the CUT homeobox family. {ECO:0000305}.
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DR EMBL; AF169227; AAF25796.1; -; mRNA.
DR EMBL; AE014135; AAF59330.2; -; Genomic_DNA.
DR RefSeq; NP_001259065.1; NM_001272136.1.
DR RefSeq; NP_524842.2; NM_080103.3.
DR AlphaFoldDB; Q9NJB5; -.
DR SMR; Q9NJB5; -.
DR BioGRID; 69903; 5.
DR IntAct; Q9NJB5; 5.
DR STRING; 7227.FBpp0302730; -.
DR PaxDb; Q9NJB5; -.
DR EnsemblMetazoa; FBtr0089104; FBpp0088173; FBgn0028996.
DR EnsemblMetazoa; FBtr0310610; FBpp0302730; FBgn0028996.
DR GeneID; 45816; -.
DR KEGG; dme:Dmel_CG1922; -.
DR UCSC; CG1922-RA; d. melanogaster.
DR CTD; 45816; -.
DR FlyBase; FBgn0028996; onecut.
DR VEuPathDB; VectorBase:FBgn0028996; -.
DR eggNOG; KOG2252; Eukaryota.
DR GeneTree; ENSGT00950000183103; -.
DR HOGENOM; CLU_296520_0_0_1; -.
DR InParanoid; Q9NJB5; -.
DR OMA; YQIVPQQ; -.
DR OrthoDB; 333224at2759; -.
DR PhylomeDB; Q9NJB5; -.
DR BioGRID-ORCS; 45816; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 45816; -.
DR PRO; PR:Q9NJB5; -.
DR Proteomes; UP000000803; Chromosome 4.
DR Bgee; FBgn0028996; Expressed in brain and 22 other tissues.
DR ExpressionAtlas; Q9NJB5; baseline and differential.
DR Genevisible; Q9NJB5; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR003350; CUT_dom.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR Pfam; PF02376; CUT; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM01109; CUT; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS51042; CUT; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Homeobox; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..1081
FT /note="Homeobox protein onecut"
FT /id="PRO_0000202407"
FT DNA_BIND 751..837
FT /note="CUT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00374"
FT DNA_BIND 918..977
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 274..326
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 481..504
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 653..688
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 844..875
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 978..1081
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 280..299
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 310..326
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 486..500
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 844..866
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1006..1041
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1058..1081
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 104
FT /note="R -> T (in Ref. 1; AAF25796)"
FT /evidence="ECO:0000305"
FT CONFLICT 132
FT /note="D -> H (in Ref. 1; AAF25796)"
FT /evidence="ECO:0000305"
FT CONFLICT 305
FT /note="R -> A (in Ref. 1; AAF25796)"
FT /evidence="ECO:0000305"
FT CONFLICT 497
FT /note="R -> K (in Ref. 1; AAF25796)"
FT /evidence="ECO:0000305"
FT CONFLICT 847
FT /note="S -> R (in Ref. 1; AAF25796)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1081 AA; 117698 MW; 2913672792F194DA CRC64;
MDSLNDIIDH QTFSQELVED ASEFITVGHH SERPSQSSQQ PNSGQDLTMS MQDIISCPVK
HRTCSASGSG SASGSDSVVM VIDALGQGNR QSAYQIVPQQ LQQRNMPLPF GLLERDRQHM
QHGREVNTSP VDFVSSDINL DGLTVDADVS QTDHSQETAV KQEQKLLIVQ SKSQDQSHRR
IRMLVDVSSV NSGLGVHVDD MDEISSDGVG CDDEGVTLSH QHLLEQEEQF GLTSHHPHLQ
PHTQIIHGLH QRSTHSEMGL DNGHGEVLSV IVHSQDSDKE DCEENDDGDA EGDLENEDDD
ERDSRSREQL LSHSSYQTLT SVNDRLSSPG FSQTSYATLT PIQPLPPIST MSEKFAYSGH
ISGGDSGDTD VNGDGAGGGV VEVGEVTNQS SEATGTVSIS SGNATSSVCS NNDCSSFSAL
SMPIGSGHLG LGVLSGVQSP FSSYEKLSSM ISPPPNNYLV SCDLHSSVSG RVINSSHLQL
SHNGNKKESG THEHTHRPAD VNGGKFSYTG HISRGDSVDN DVNGEKFSFS DHISGGDSGD
EDANREKFIY SDHISEGENG PDVNSGTNWL QMHSEREVRL HMPVPAELEA RFHISSERRT
RLNVPPARGS LSRHLAPNAP ICPADWKADD WKHSNAGVVS LTADMPVVVS LTPTPPPLRD
DSVSGIKQNE RSSPGHREQY FLDKSQEPSS VVADQCSPGI NGTPQSVCVI HQQSPSALGN
GFQSSSQQAK VSSCASPKGT VSSGGAVSNR IANSSDMEEI NTKDLAQRIS AELKRYSIPQ
AIFAQRVLCR SQGTLSDLLR NPKPWSKLKS GRETFRRMYK WLQEPEFQRM SALRMAAAQI
PQRAPLSSGM SLGSATGPSG STGTIPTDLD PHGGPTMIQN PLTNESDSSP ASTPVTSVLV
GSVVSCRRKE EPQIEQMPQP KKPRLVFTDL QRRTLQAIFK ETKRPSKEMQ VTIARQLGLE
PTTVGNFFMN ARRRSMDKWR DDDSKSTMHV AHSRQQQDEQ QKDNAHTHSQ SQIQDQNHSQ
NQAIHNSMSQ DPYSNLHTTA MSPLGAFDED ADMDLELESH DFDLVDPDEH GDTNDPNGDM
L
