OOEP_CANLF
ID OOEP_CANLF Reviewed; 149 AA.
AC Q0ZFW8;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=Oocyte-expressed protein;
DE AltName: Full=Oocyte- and embryo-specific protein 19;
DE Short=dOEP19;
GN Name=OOEP; Synonyms=OEP19;
OS Canis lupus familiaris (Dog) (Canis familiaris).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX NCBI_TaxID=9615;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Oocyte;
RA He W., Li Y., Chertihin O., Herr J.C.;
RT "Cloning the orthologous gene of moep 19 in dog.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: As part of the OOEP-KHDC3 scaffold, recruits BLM and TRIM25
CC to DNA replication forks, thereby promoting the ubiquitination of BLM
CC by TRIM25, enhancing BLM retainment at replication forks and therefore
CC promoting stalled replication fork restart (By similarity). Positively
CC regulates the homologous recombination-mediated DNA double-strand break
CC (DSB) repair pathway by regulating ATM activation and RAD51 recruitment
CC to DSBs in oocytes (By similarity). Thereby contributes to oocyte
CC survival and the resumption and completion of meiosis (By similarity).
CC As a member of the subcortical maternal complex (SCMC), plays an
CC essential role for zygotes to progress beyond the first embryonic cell
CC divisions via regulation of actin dynamics (By similarity). Required
CC for the formation of F-actin cytoplasmic lattices in oocytes which in
CC turn are responsible for symmetric division of zygotes via the
CC regulation of mitotic spindle formation and positioning (By
CC similarity). {ECO:0000250|UniProtKB:Q9CWE6}.
CC -!- SUBUNIT: Component of the subcortical maternal complex (SCMC), at least
CC composed of NLRP5, KHDC3, OOEP, and TLE6 (By similarity). Within the
CC complex, interacts with NLRP5, KHDC3 and TLE6 (By similarity). As part
CC of the SCMC interacts with the SCMC-associated protein NLRP4F (By
CC similarity). The SCMC may facilitate translocation of its components
CC between the nuclear and cytoplasmic compartments (By similarity). Forms
CC a scaffold complex with KHDC3/FILIA, and interacts with BLM and TRIM25
CC at DNA replication forks (By similarity).
CC {ECO:0000250|UniProtKB:A6NGQ2, ECO:0000250|UniProtKB:Q9CWE6}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9CWE6}. Nucleus
CC {ECO:0000250|UniProtKB:Q9CWE6}.
CC -!- DOMAIN: Contains an atypical KH domain with amino acid changes at
CC critical sites, suggesting that it may not bind RNA.
CC -!- SIMILARITY: Belongs to the KHDC1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DQ470473; ABF13465.1; -; mRNA.
DR RefSeq; NP_001106928.1; NM_001113457.1.
DR AlphaFoldDB; Q0ZFW8; -.
DR SMR; Q0ZFW8; -.
DR STRING; 9612.ENSCAFP00000033796; -.
DR PaxDb; Q0ZFW8; -.
DR Ensembl; ENSCAFT00030025423; ENSCAFP00030022200; ENSCAFG00030013739.
DR Ensembl; ENSCAFT00040032918; ENSCAFP00040028643; ENSCAFG00040017820.
DR Ensembl; ENSCAFT00845021225; ENSCAFP00845016689; ENSCAFG00845011927.
DR GeneID; 474966; -.
DR KEGG; cfa:474966; -.
DR CTD; 441161; -.
DR VEuPathDB; HostDB:ENSCAFG00845011927; -.
DR eggNOG; ENOG502RU0M; Eukaryota.
DR GeneTree; ENSGT00940000162097; -.
DR HOGENOM; CLU_146793_0_0_1; -.
DR InParanoid; Q0ZFW8; -.
DR OMA; RVRPWWF; -.
DR OrthoDB; 1476034at2759; -.
DR TreeFam; TF338690; -.
DR Proteomes; UP000002254; Chromosome 12.
DR Bgee; ENSCAFG00000024700; Expressed in placenta and 47 other tissues.
DR GO; GO:0045179; C:apical cortex; IBA:GO_Central.
DR GO; GO:0005938; C:cell cortex; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0032991; C:protein-containing complex; IBA:GO_Central.
DR GO; GO:0106333; C:subcortical maternal complex; IEA:Ensembl.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0007015; P:actin filament organization; ISS:UniProtKB.
DR GO; GO:0009880; P:embryonic pattern specification; IBA:GO_Central.
DR GO; GO:0051293; P:establishment of spindle localization; ISS:UniProtKB.
DR GO; GO:0035088; P:establishment or maintenance of apical/basal cell polarity; IBA:GO_Central.
DR GO; GO:2000781; P:positive regulation of double-strand break repair; ISS:UniProtKB.
DR GO; GO:1905168; P:positive regulation of double-strand break repair via homologous recombination; ISS:UniProtKB.
DR GO; GO:0045836; P:positive regulation of meiotic nuclear division; ISS:UniProtKB.
DR GO; GO:0051302; P:regulation of cell division; ISS:UniProtKB.
DR GO; GO:0070201; P:regulation of establishment of protein localization; ISS:UniProtKB.
DR GO; GO:0032880; P:regulation of protein localization; ISS:UniProtKB.
DR GO; GO:0031297; P:replication fork processing; ISS:UniProtKB.
DR CDD; cd12795; FILIA_N_like; 1.
DR Gene3D; 3.30.1370.10; -; 1.
DR InterPro; IPR036612; KH_dom_type_1_sf.
DR InterPro; IPR031952; MOEP19_KH-like.
DR InterPro; IPR040068; OOEP.
DR PANTHER; PTHR19447:SF14; PTHR19447:SF14; 1.
DR Pfam; PF16005; MOEP19; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..149
FT /note="Oocyte-expressed protein"
FT /id="PRO_0000328800"
FT DOMAIN 49..110
FT /note="KH; atypical"
SQ SEQUENCE 149 AA; 17068 MW; B04B0647D6192E31 CRC64;
MVDDAGAAEV LGDEWRPAWS LDRLLRAPLP PPRIRTRPWW FPVQELRDPL VFYLEAWLAD
AIFGPDRAII PEIEWMSQVL LTVDVVNSGD LVEISIFGWP RVQNRVKSVL LSLASWHRKH
RARAEKMKQL EEFLKTGGTG PQTPEHPVA
