OP10_MAIZE
ID OP10_MAIZE Reviewed; 1059 AA.
AC P0DKL2; A0A096TBT9; A0A096TBU0; A0A096TBU1;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2016, sequence version 1.
DT 25-MAY-2022, entry version 30.
DE RecName: Full=Protein OPAQUE10 {ECO:0000303|PubMed:27541862};
GN Name=O10 {ECO:0000303|PubMed:27541862};
GN ORFNames=GRMZM2G346263 {ECO:0000305}, Zm.12817;
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. B73;
RX PubMed=19965430; DOI=10.1126/science.1178534;
RA Schnable P.S., Ware D., Fulton R.S., Stein J.C., Wei F., Pasternak S.,
RA Liang C., Zhang J., Fulton L., Graves T.A., Minx P., Reily A.D.,
RA Courtney L., Kruchowski S.S., Tomlinson C., Strong C., Delehaunty K.,
RA Fronick C., Courtney B., Rock S.M., Belter E., Du F., Kim K., Abbott R.M.,
RA Cotton M., Levy A., Marchetto P., Ochoa K., Jackson S.M., Gillam B.,
RA Chen W., Yan L., Higginbotham J., Cardenas M., Waligorski J., Applebaum E.,
RA Phelps L., Falcone J., Kanchi K., Thane T., Scimone A., Thane N., Henke J.,
RA Wang T., Ruppert J., Shah N., Rotter K., Hodges J., Ingenthron E.,
RA Cordes M., Kohlberg S., Sgro J., Delgado B., Mead K., Chinwalla A.,
RA Leonard S., Crouse K., Collura K., Kudrna D., Currie J., He R.,
RA Angelova A., Rajasekar S., Mueller T., Lomeli R., Scara G., Ko A.,
RA Delaney K., Wissotski M., Lopez G., Campos D., Braidotti M., Ashley E.,
RA Golser W., Kim H., Lee S., Lin J., Dujmic Z., Kim W., Talag J., Zuccolo A.,
RA Fan C., Sebastian A., Kramer M., Spiegel L., Nascimento L., Zutavern T.,
RA Miller B., Ambroise C., Muller S., Spooner W., Narechania A., Ren L.,
RA Wei S., Kumari S., Faga B., Levy M.J., McMahan L., Van Buren P.,
RA Vaughn M.W., Ying K., Yeh C.-T., Emrich S.J., Jia Y., Kalyanaraman A.,
RA Hsia A.-P., Barbazuk W.B., Baucom R.S., Brutnell T.P., Carpita N.C.,
RA Chaparro C., Chia J.-M., Deragon J.-M., Estill J.C., Fu Y., Jeddeloh J.A.,
RA Han Y., Lee H., Li P., Lisch D.R., Liu S., Liu Z., Nagel D.H., McCann M.C.,
RA SanMiguel P., Myers A.M., Nettleton D., Nguyen J., Penning B.W.,
RA Ponnala L., Schneider K.L., Schwartz D.C., Sharma A., Soderlund C.,
RA Springer N.M., Sun Q., Wang H., Waterman M., Westerman R., Wolfgruber T.K.,
RA Yang L., Yu Y., Zhang L., Zhou S., Zhu Q., Bennetzen J.L., Dawe R.K.,
RA Jiang J., Jiang N., Presting G.G., Wessler S.R., Aluru S.,
RA Martienssen R.A., Clifton S.W., McCombie W.R., Wing R.A., Wilson R.K.;
RT "The B73 maize genome: complexity, diversity, and dynamics.";
RL Science 326:1112-1115(2009).
RN [2]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBUNIT, DOMAIN,
RP SUBCELLULAR LOCATION, AND INTERACTION WITH ZEINS; FL1 AND HIP.
RX PubMed=27541862; DOI=10.1371/journal.pgen.1006270;
RA Yao D., Qi W., Li X., Yang Q., Yan S., Ling H., Wang G., Wang G., Song R.;
RT "Maize opaque10 encodes a cereal-specific protein that is essential for the
RT proper distribution of zeins in endosperm protein bodies.";
RL PLoS Genet. 12:E1006270-E1006270(2016).
CC -!- FUNCTION: Cereal endosperm protein required for the ring-shaped
CC distribution of 22 kDa alpha- and 16 kDa gamma-zeins in protein bodies.
CC {ECO:0000269|PubMed:27541862}.
CC -!- SUBUNIT: Homodimer (PubMed:27541862). Interacts (via N-terminus) with
CC FL1 (via C-terminus), HIP, 19 kDa alpha-zein (AC P06677), 22 kDa alpha-
CC zein (AC O48966), 16 kDa gamma-zein (AC P08031) and 50 kDa gamma-zein
CC (AC C0P381) (PubMed:27541862). {ECO:0000269|PubMed:27541862}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:27541862}; Single-pass membrane protein
CC {ECO:0000255}. Note=In endosperm, predominantly deposited into protein
CC bodies, at the interface between the alpha-zein-rich region and the
CC gamma-zein-rich region. {ECO:0000269|PubMed:27541862}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=P0DKL2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P0DKL2-2; Sequence=VSP_058623;
CC Name=3;
CC IsoId=P0DKL2-3; Sequence=VSP_058623, VSP_058624;
CC Name=4;
CC IsoId=P0DKL2-4; Sequence=VSP_058621, VSP_058622;
CC -!- TISSUE SPECIFICITY: Expressed in kernels.
CC {ECO:0000269|PubMed:27541862}.
CC -!- DEVELOPMENTAL STAGE: Expressed continuously during kernel development.
CC {ECO:0000269|PubMed:27541862}.
CC -!- DOMAIN: The seven repeat domain is responsible for dimerization.
CC {ECO:0000269|PubMed:27541862}.
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DR EMBL; AC204586; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_008665265.1; XM_008667043.1.
DR AlphaFoldDB; P0DKL2; -.
DR EnsemblPlants; Zm00001eb054280_T001; Zm00001eb054280_P001; Zm00001eb054280. [P0DKL2-1]
DR Gramene; Zm00001eb054280_T001; Zm00001eb054280_P001; Zm00001eb054280. [P0DKL2-1]
DR eggNOG; ENOG502R1IX; Eukaryota.
DR Proteomes; UP000007305; Chromosome 1.
DR ExpressionAtlas; P0DKL2; baseline and differential.
DR GO; GO:0005884; C:actin filament; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0008093; F:cytoskeletal anchor activity; IBA:GO_Central.
DR GO; GO:0051764; P:actin crosslink formation; IBA:GO_Central.
DR Gene3D; 1.10.418.10; -; 1.
DR InterPro; IPR036872; CH_dom_sf.
DR SUPFAM; SSF47576; SSF47576; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Endoplasmic reticulum; Membrane; Reference proteome;
KW Repeat; Transmembrane; Transmembrane helix.
FT CHAIN 1..1059
FT /note="Protein OPAQUE10"
FT /id="PRO_0000438208"
FT TRANSMEM 1003..1023
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 269..342
FT /note="1"
FT /evidence="ECO:0000305"
FT REPEAT 343..416
FT /note="2"
FT /evidence="ECO:0000305"
FT REPEAT 417..490
FT /note="3"
FT /evidence="ECO:0000305"
FT REPEAT 491..564
FT /note="4"
FT /evidence="ECO:0000305"
FT REPEAT 565..638
FT /note="5"
FT /evidence="ECO:0000305"
FT REPEAT 639..712
FT /note="6"
FT /evidence="ECO:0000305"
FT REPEAT 713..786
FT /note="7"
FT /evidence="ECO:0000305"
FT REGION 269..786
FT /note="7 X approximate repeats"
FT /evidence="ECO:0000305"
FT REGION 511..534
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 732..756
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 856..875
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 889..998
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 520..534
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 905..926
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 941..978
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 151..167
FT /note="ICQILGLAGIDLFTPSD -> VWIFAHFPSRRFGSINI (in isoform
FT 4)"
FT /id="VSP_058621"
FT VAR_SEQ 168..1059
FT /note="Missing (in isoform 4)"
FT /id="VSP_058622"
FT VAR_SEQ 294..513
FT /note="Missing (in isoform 2 and isoform 3)"
FT /id="VSP_058623"
FT VAR_SEQ 1020..1059
FT /note="SAALLQKKQGGKLCDGHTISFGKICSEQRKGQEHGQRKRP -> RRSKGGSF
FT ATVIPSLSGKSARSNARARNMGRENVPDAYPGGRLKA (in isoform 3)"
FT /id="VSP_058624"
SQ SEQUENCE 1059 AA; 114580 MW; 6444D4BD5F9803C6 CRC64;
MSLHAARGGP HEDLSWSAIG RRAPRDVVTF GDREGGGGMR SAQQGPGAVR VDEASSASTF
RELDEAFLQT QTKIWLGEVL HLRFGEDALV ADLLADGELL FQVSKVLWKM LLKNNREQLK
QSKVYIYERL SFGRSSGKYM PYSKVDSFLK ICQILGLAGI DLFTPSDVVE KRNVRKVCIC
IRSVSKKSLI LHLNVPDFDV VTYTISMPNY VVGGIRRNLE QTQYSSSSSS GYSPCARSKV
LQQQIIFGGQ NDQHEDTHYD SDEAESKLSL LEPEDSVNED NFAAVLSQFN DAHNKGSEGY
GESGCGKHGE KSLAESVGSL NIGIIDFEFM DSTPLIHDKE SCSLLEPEDS VNEDNFAAVL
SKFNDAPNKE SKGYGESGRG KHGEKSLAES VGSLNIGIVD SEFMDSSPLI HDKESCSPLE
PEGSVNEDNF TAVLSQFNDA PNKESEGYGE SGCGKHGEKS LVESVGSLNI GIVDSEFMDS
SPLIHDKESC SPLEPEDSVN EDNFAAVLSQ FNDAPNKESE GYGESGRGKH GEKSLAESVG
SLNIGIIDSE FMDSSPLIHD KESCFPLEPE GSVNEDNFTA VLSQFNDAPN KESEGYGESG
CGKHGEKSLV ESVGSLNIGI VDSEFMDSSP LIHDKESCSP LEPEDSVNED NFTAVLSQFN
DAPNKESEAY GESGCGKHGE NSLDESVGSL NIGVIDSEFM DSSPLIHDKE SCSPLEPEDS
VNEDNFAAVL SQYSDGPNEG NEGYGESGHY KHEEKSLDES VGSLTIGIID SEFMDSSRPI
HDKESCSTGS AADQCSRTIP AKYELSSEES DSTGSHLVFD SGKNYLELNN HSVTDLERIY
NGHATSVDQY VRGNGETLAD HPKKEEAGLQ KDTGTIAQHR DTLACDGESV CSSCEEPRRG
LNGEPSDFSS ESHSRLTPTH NTGGKLSMVS EHPVHNMESD MTGMASDSTN PELNPEASTR
NEMDGSRSTD NPVEPENVAQ DSATRGRPEG DAPRSGKGVL RSVAGGITLV GAVFFMFHLS
AALLQKKQGG KLCDGHTISF GKICSEQRKG QEHGQRKRP
