OP1S2_DANRE
ID OP1S2_DANRE Reviewed; 354 AA.
AC Q9W6A8;
DT 27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Opsin-1, short-wave-sensitive 2;
DE AltName: Full=Blue cone photoreceptor pigment;
DE AltName: Full=Blue-sensitive opsin;
DE AltName: Full=Opsin SWS-2;
GN Name=opn1sw2; Synonyms=bluops, opn1sw1, sws2;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Eye;
RX PubMed=10349976; DOI=10.1017/s0952523899163168;
RA Vihtelic T.S., Doro C.J., Hyde D.R.;
RT "Cloning and characterization of six zebrafish photoreceptor opsin cDNAs
RT and immunolocalization of their corresponding proteins.";
RL Vis. Neurosci. 16:571-585(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND BIOPHYSICOCHEMICAL
RP PROPERTIES.
RC STRAIN=AB; TISSUE=Eye;
RX PubMed=12618404; DOI=10.1093/genetics/163.2.663;
RA Chinen A., Hamaoka T., Yamada Y., Kawamura S.;
RT "Gene duplication and spectral diversification of cone visual pigments of
RT zebrafish.";
RL Genetics 163:663-675(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Retina;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Visual pigments are the light-absorbing molecules that
CC mediate vision. They consist of an apoprotein, opsin, covalently linked
CC to cis-retinal.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Absorption:
CC Abs(max)=416 nm {ECO:0000269|PubMed:12618404};
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Retinal long single cone outer segments.
CC {ECO:0000269|PubMed:10349976}.
CC -!- PTM: Phosphorylated on some or all of the serine and threonine residues
CC present in the C-terminal region. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Opsin
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AF109372; AAD24755.1; -; mRNA.
DR EMBL; AB087809; BAC24133.1; -; Genomic_DNA.
DR EMBL; AL844847; CAE30415.1; -; Genomic_DNA.
DR EMBL; BC059418; AAH59418.1; -; mRNA.
DR EMBL; BC062277; AAH62277.1; -; mRNA.
DR RefSeq; NP_571267.1; NM_131192.1.
DR AlphaFoldDB; Q9W6A8; -.
DR SMR; Q9W6A8; -.
DR STRING; 7955.ENSDARP00000019477; -.
DR PaxDb; Q9W6A8; -.
DR Ensembl; ENSDART00000011178; ENSDARP00000019477; ENSDARG00000017274.
DR Ensembl; ENSDART00000192024; ENSDARP00000144766; ENSDARG00000115330.
DR GeneID; 30435; -.
DR KEGG; dre:30435; -.
DR CTD; 30435; -.
DR ZFIN; ZDB-GENE-990604-40; opn1sw2.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01030000234549; -.
DR InParanoid; Q9W6A8; -.
DR OMA; CFCFGFP; -.
DR OrthoDB; 940057at2759; -.
DR PhylomeDB; Q9W6A8; -.
DR TreeFam; TF324998; -.
DR PRO; PR:Q9W6A8; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 11.
DR Bgee; ENSDARG00000017274; Expressed in retina and 14 other tissues.
DR ExpressionAtlas; Q9W6A8; baseline.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0001750; C:photoreceptor outer segment; IDA:ZFIN.
DR GO; GO:0009882; F:blue light photoreceptor activity; NAS:UniProtKB.
DR GO; GO:0008020; F:G protein-coupled photoreceptor activity; IBA:GO_Central.
DR GO; GO:0071482; P:cellular response to light stimulus; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007602; P:phototransduction; IBA:GO_Central.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR001760; Opsin.
DR InterPro; IPR001521; Opsin_blue.
DR InterPro; IPR027430; Retinal_BS.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00238; OPSIN.
DR PRINTS; PR00574; OPSINBLUE.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR PROSITE; PS00238; OPSIN; 1.
PE 1: Evidence at protein level;
KW Chromophore; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Phosphoprotein; Photoreceptor protein; Receptor;
KW Reference proteome; Retinal protein; Sensory transduction; Transducer;
KW Transmembrane; Transmembrane helix; Vision.
FT CHAIN 1..354
FT /note="Opsin-1, short-wave-sensitive 2"
FT /id="PRO_0000197755"
FT TOPO_DOM 1..43
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..68
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 69..80
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..106
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 107..120
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..140
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 141..159
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..183
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 184..209
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..237
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 238..259
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..283
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 284..291
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..316
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 317..354
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 303
FT /note="N6-(retinylidene)lysine"
FT /evidence="ECO:0000250"
FT CARBOHYD 24
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 207
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 117..194
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 354 AA; 39484 MW; A434A07E864A30EC CRC64;
MKQQQQTPEL FEDFHMPITL DVSNISAYSP FLVPQDHLGH SGVFMGMSAF MLFLFIAGTA
INVLTIVCTI QYKKLRSHLN YILVNLAISN LWVSVFGSSV AFYAFYKKYF VFGPIGCKIE
GFTSTIGGMV SLWSLAVVAL ERWLVICKPL GNFTFKTPHA IAGCILPWCM ALAAGLPPLL
GWSRYIPEGL QCSCGPDWYT TNNKFNNESY VMFLFCFCFA VPFSTIVFCY GQLLITLKLA
AKAQADSAST QKAEREVTKM VVVMVFGFLI CWGPYAIFAI WVVSNRGAPF DLRLATIPSC
LCKASTVYNP VIYVLMNKQF RSCMMKMVFN KNIEEDEASS SSQVTQVSSV APEK
