OP21A_ARATH
ID OP21A_ARATH Reviewed; 167 AA.
AC Q6ID99; Q56ZY5; Q9LM70;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Outer envelope pore protein 21A, chloroplastic;
DE AltName: Full=Chloroplastic outer envelope pore protein of 21 kDa A;
GN Name=OEP21A; OrderedLocusNames=At1g20816; ORFNames=F2D10.33;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 37-167.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=12649433; DOI=10.1110/ps.0237503;
RA Schleiff E., Eichacker L.A., Eckart K., Becker T., Mirus O., Stahl T.,
RA Soll J.;
RT "Prediction of the plant beta-barrel proteome: a case study of the
RT chloroplast outer envelope.";
RL Protein Sci. 12:748-759(2003).
CC -!- FUNCTION: Voltage-dependent rectifying anion channel that facilitates
CC the translocation between chloroplast and cytoplasm of phosphorylated
CC carbohydrates such as triosephosphate, 3-phosphoglycerate and inorganic
CC phosphate (Pi) depending of ATP to triosephosphate ratio in the
CC plastidial intermembrane space; in high triosephosphate/ATP conditions
CC (e.g. photosynthesis), export of triosphosphate from chloroplast
CC (outward rectifying channels), but in high ATP/triosephosphate
CC conditions (e.g. dark phase), import of phosphosolutes (inward
CC rectifying channels) (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, etioplast membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}. Plastid, chloroplast outer
CC membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC Note=Present in non-green root plastids. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the plastid outer envelope porin OEP21 (TC
CC 1.B.29) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF80623.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAD94125.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC069251; AAF80623.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30027.1; -; Genomic_DNA.
DR EMBL; BT014774; AAT41757.1; -; mRNA.
DR EMBL; BT015010; AAT70461.1; -; mRNA.
DR EMBL; AK220825; BAD94125.1; ALT_INIT; mRNA.
DR RefSeq; NP_173505.2; NM_101934.3.
DR AlphaFoldDB; Q6ID99; -.
DR SMR; Q6ID99; -.
DR STRING; 3702.AT1G20816.1; -.
DR TCDB; 1.B.29.1.4; the plastid outer envelope porin of 21 kda (oep21) family.
DR PaxDb; Q6ID99; -.
DR PRIDE; Q6ID99; -.
DR ProteomicsDB; 248898; -.
DR EnsemblPlants; AT1G20816.1; AT1G20816.1; AT1G20816.
DR GeneID; 838673; -.
DR Gramene; AT1G20816.1; AT1G20816.1; AT1G20816.
DR KEGG; ath:AT1G20816; -.
DR Araport; AT1G20816; -.
DR TAIR; locus:2825702; AT1G20816.
DR eggNOG; ENOG502RX9S; Eukaryota.
DR HOGENOM; CLU_104399_0_0_1; -.
DR InParanoid; Q6ID99; -.
DR OMA; YFFHEAS; -.
DR OrthoDB; 1516696at2759; -.
DR PhylomeDB; Q6ID99; -.
DR PRO; PR:Q6ID99; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q6ID99; baseline and differential.
DR Genevisible; Q6ID99; AT.
DR GO; GO:0034426; C:etioplast membrane; ISS:UniProtKB.
DR GO; GO:0031359; C:integral component of chloroplast outer membrane; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR GO; GO:0015288; F:porin activity; ISS:UniProtKB.
DR GO; GO:0008308; F:voltage-gated anion channel activity; ISS:UniProtKB.
DR GO; GO:0044070; P:regulation of anion transport; ISS:UniProtKB.
DR InterPro; IPR034575; OEP21.
DR PANTHER; PTHR35993; PTHR35993; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Ion transport; Membrane; Plastid; Plastid outer membrane;
KW Porin; Reference proteome; Transmembrane; Transmembrane beta strand;
KW Transport.
FT CHAIN 1..167
FT /note="Outer envelope pore protein 21A, chloroplastic"
FT /id="PRO_0000415547"
FT TOPO_DOM 1..21
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 22..31
FT /note="Beta stranded; Name=1"
FT /evidence="ECO:0000250"
FT TOPO_DOM 32..55
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..65
FT /note="Beta stranded; Name=2"
FT /evidence="ECO:0000250"
FT TOPO_DOM 66..71
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..81
FT /note="Beta stranded; Name=3"
FT /evidence="ECO:0000250"
FT TOPO_DOM 82..87
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..97
FT /note="Beta stranded; Name=4"
FT /evidence="ECO:0000250"
FT TOPO_DOM 98..110
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..120
FT /note="Beta stranded; Name=5"
FT /evidence="ECO:0000250"
FT TOPO_DOM 121..127
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..137
FT /note="Beta stranded; Name=6"
FT /evidence="ECO:0000250"
FT TOPO_DOM 138..142
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..152
FT /note="Beta stranded; Name=7"
FT /evidence="ECO:0000250"
FT TOPO_DOM 153..158
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..167
FT /note="Beta stranded; Name=8"
FT /evidence="ECO:0000250"
FT CONFLICT 135
FT /note="Y -> N (in Ref. 4; BAD94125)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 167 AA; 19782 MW; 0705A3F316470572 CRC64;
METSLRYATN SRSLKIHAKE KFPVNSKTRL QLHGELDTGA GVPSYFCAMI RYFFHEASTN
LGVGLHYDKR EKLRCLVRGK KKFPVITDEV VTFNIKGRCD FDQDLVQRNA KGAAEFDWNI
WKFQKDQDLR LRIGYEMFEK VPYMQIRENN WTFNTNLKGK WNVRYDL
