OPALI_MOUSE
ID OPALI_MOUSE Reviewed; 143 AA.
AC Q7M750; Q6P903;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2003, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Opalin {ECO:0000303|PubMed:17442045};
DE AltName: Full=Oligodendrocytic myelin paranodal and inner loop protein;
DE AltName: Full=Transmembrane protein 10 {ECO:0000303|PubMed:18439243};
GN Name=Opalin; Synonyms=Tmem10;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP IDENTIFICATION.
RX PubMed=11814680; DOI=10.1016/s0378-1119(01)00846-0;
RA Nobile C., Hinzmann B., Scannapieco P., Siebert R., Zimbello R.,
RA Perez-Tur J., Sarafidou T., Moschonas N.K., French L., Deloukas P.,
RA Ciccodicola A., Gesk S., Poza J.J., Lo Nigro C., Seri M.,
RA Schlegelberger B., Rosenthal A., Valle G., Lopez de Munain A.,
RA Tassinari C.A., Michelucci R.;
RT "Identification and characterization of a novel human brain-specific gene,
RT homologous to S. scrofa tmp83.5, in the chromosome 10q24 critical region
RT for temporal lobe epilepsy and spastic paraplegia.";
RL Gene 282:87-94(2002).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=17442045; DOI=10.1111/j.1471-4159.2007.04583.x;
RA Aruga J., Yoshikawa F., Nozaki Y., Sakaki Y., Toyoda A., Furuichi T.;
RT "An oligodendrocyte enhancer in a phylogenetically conserved intron region
RT of the mammalian myelin gene Opalin.";
RL J. Neurochem. 102:1533-1547(2007).
RN [4]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=18439243; DOI=10.1186/1471-2202-9-40;
RA Kippert A., Trajkovic K., Fitzner D., Opitz L., Simons M.;
RT "Identification of Tmem10/Opalin as a novel marker for oligodendrocytes
RT using gene expression profiling.";
RL BMC Neurosci. 9:40-40(2008).
RN [5]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP GLYCOSYLATION AT ASN-6; ASN-12 AND THR-14, AND MUTAGENESIS OF ASN-6; ASN-12
RP AND THR-14.
RX PubMed=18490449; DOI=10.1074/jbc.m801314200;
RA Yoshikawa F., Sato Y., Tohyama K., Akagi T., Hashikawa T.,
RA Nagakura-Takagi Y., Sekine Y., Morita N., Baba H., Suzuki Y., Sugano S.,
RA Sato A., Furuichi T.;
RT "Opalin, a transmembrane sialylglycoprotein located in the central nervous
RT system myelin paranodal loop membrane.";
RL J. Biol. Chem. 283:20830-20840(2008).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [7]
RP DISRUPTION PHENOTYPE.
RX PubMed=27855200; DOI=10.1371/journal.pone.0166732;
RA Yoshikawa F., Sato Y., Tohyama K., Akagi T., Furuse T., Sadakata T.,
RA Tanaka M., Shinoda Y., Hashikawa T., Itohara S., Sano Y., Ghandour M.S.,
RA Wakana S., Furuichi T.;
RT "Mammalian-Specific Central Myelin Protein Opalin Is Redundant for Normal
RT Myelination: Structural and Behavioral Assessments.";
RL PLoS ONE 11:E0166732-E0166732(2016).
RN [8]
RP DEVELOPMENTAL STAGE, FUNCTION, TISSUE SPECIFICITY, AND SUBCELLULAR
RP LOCATION.
RX PubMed=30837646; DOI=10.1038/s41598-019-40342-x;
RA de Faria O. Jr., Dhaunchak A.S., Kamen Y., Roth A.D., Kuhlmann T.,
RA Colman D.R., Kennedy T.E.;
RT "TMEM10 Promotes Oligodendrocyte Differentiation and is Expressed by
RT Oligodendrocytes in Human Remyelinating Multiple Sclerosis Plaques.";
RL Sci. Rep. 9:3606-3606(2019).
CC -!- FUNCTION: Central nervous system-specific myelin protein that increase
CC myelin genes expression during oligodendrocyte differentiation.
CC Promotes oligodendrocyte terminal differentiation.
CC {ECO:0000269|PubMed:30837646}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:18439243,
CC ECO:0000269|PubMed:18490449, ECO:0000269|PubMed:30837646}; Single-pass
CC type I membrane protein {ECO:0000255}. Note=In the CNS, enriched in the
CC myelin paranodal and inner loop membranes, but not that of the PNS.
CC Enriched in the leading edge of extending processes (PubMed:30837646).
CC {ECO:0000269|PubMed:30837646}.
CC -!- TISSUE SPECIFICITY: Expressed specifically in oligodendrocytes of the
CC brain. {ECO:0000269|PubMed:17442045, ECO:0000269|PubMed:18439243,
CC ECO:0000269|PubMed:18490449, ECO:0000269|PubMed:30837646}.
CC -!- DEVELOPMENTAL STAGE: Expression in the cerebellum increases
CC dramatically after the first postnatal week (PubMed:18490449,
CC PubMed:30837646). Selectively expressed in brain at P21, and not
CC expressed in any other tissues tested including thymus, lung, heart,
CC liver, spleen, kidney and testis. {ECO:0000269|PubMed:18490449,
CC ECO:0000269|PubMed:30837646}.
CC -!- INDUCTION: By leukemia inhibitory factor (LIF) and cAMP analogs.
CC Suppressed in the presence of astrocytic or neural-stem-like
CC differentiation factors such as bone morphotic protein (BMP) and fetal
CC calf serum. {ECO:0000269|PubMed:17442045}.
CC -!- DISRUPTION PHENOTYPE: Deficient mice are born the expected Mendelian
CC ratio and have a normal body shape and weight. No obvious abnormalities
CC in gross anatomy or major myelin protein components nor in the
CC compartment of myelinated axons and oligodendrocyte maturation.
CC Nevertheless, deficient mice display increased exploratory activity in
CC a novel environment. {ECO:0000269|PubMed:27855200}.
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DR EMBL; BC060980; AAH60980.1; -; mRNA.
DR EMBL; BK000002; DAA00020.1; -; mRNA.
DR CCDS; CCDS37985.1; -.
DR RefSeq; NP_705740.1; NM_153520.1.
DR AlphaFoldDB; Q7M750; -.
DR STRING; 10090.ENSMUSP00000084422; -.
DR GlyGen; Q7M750; 3 sites.
DR iPTMnet; Q7M750; -.
DR PhosphoSitePlus; Q7M750; -.
DR PaxDb; Q7M750; -.
DR PRIDE; Q7M750; -.
DR ProteomicsDB; 293931; -.
DR Antibodypedia; 2734; 41 antibodies from 11 providers.
DR DNASU; 226115; -.
DR Ensembl; ENSMUST00000087176; ENSMUSP00000084422; ENSMUSG00000050121.
DR GeneID; 226115; -.
DR KEGG; mmu:226115; -.
DR UCSC; uc008hlq.1; mouse.
DR CTD; 93377; -.
DR MGI; MGI:2657025; Opalin.
DR VEuPathDB; HostDB:ENSMUSG00000050121; -.
DR eggNOG; ENOG502RWAT; Eukaryota.
DR GeneTree; ENSGT00390000009395; -.
DR HOGENOM; CLU_1824653_0_0_1; -.
DR InParanoid; Q7M750; -.
DR OMA; DCGPSVG; -.
DR OrthoDB; 1457772at2759; -.
DR PhylomeDB; Q7M750; -.
DR TreeFam; TF337818; -.
DR BioGRID-ORCS; 226115; 1 hit in 70 CRISPR screens.
DR PRO; PR:Q7M750; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q7M750; protein.
DR Bgee; ENSMUSG00000050121; Expressed in lumbar subsegment of spinal cord and 65 other tissues.
DR ExpressionAtlas; Q7M750; baseline and differential.
DR Genevisible; Q7M750; MM.
DR GO; GO:0044291; C:cell-cell contact zone; IDA:CACAO.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0048713; P:regulation of oligodendrocyte differentiation; IMP:UniProtKB.
DR InterPro; IPR026609; Opalin.
DR PANTHER; PTHR21102; PTHR21102; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..143
FT /note="Opalin"
FT /id="PRO_0000072592"
FT TOPO_DOM 1..30
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 52..143
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 79..103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 79..95
FT /note="Required for plasma membrane localization"
FT /evidence="ECO:0000269|PubMed:30837646"
FT CARBOHYD 6
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:18490449"
FT CARBOHYD 12
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:18490449"
FT CARBOHYD 14
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000269|PubMed:18490449"
FT MUTAGEN 6
FT /note="N->Q: Impairs cell surface expression."
FT /evidence="ECO:0000269|PubMed:18490449"
FT MUTAGEN 12
FT /note="N->Q: Impairs cell surface expression."
FT /evidence="ECO:0000269|PubMed:18490449"
FT MUTAGEN 14
FT /note="T->A: Impairs cell surface expression."
FT /evidence="ECO:0000269|PubMed:18490449"
FT CONFLICT 133
FT /note="L -> F (in Ref. 1; AAH60980)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 143 AA; 15833 MW; A5DF69DB05C72B41 CRC64;
MSFSLNFTLP SNTTSSPVVT SAKATDCGPS IGLAAGIPSL LATALLVALL FTLIQRRRTI
DDEPVEETEI PCEISELYDN PKISENPRRS PTHEMNPRGS QEGHIYVKTV SGSEEPLPDT
YRPPEELERR RGLWWLVPSL SLE
