OPALI_PIG
ID OPALI_PIG Reviewed; 142 AA.
AC Q29102;
DT 10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Opalin;
DE AltName: Full=Oligodendrocytic myelin paranodal and inner loop protein;
DE AltName: Full=Transmembrane protein 10;
DE AltName: Full=Transmembrane protein sp83.5;
GN Name=OPALIN; Synonyms=SP83.5, TMEM10;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, ALTERNATIVE SPLICING, AND
RP SUBCELLULAR LOCATION.
RC TISSUE=Brain;
RX PubMed=9746342; DOI=10.1046/j.1432-1327.1998.2560024.x;
RA Bangsow T., Schepelmann S., Martin C., May M., Oberthuer A., Perl S.,
RA Knuepfer E., Zinke H., Gassen H.G.;
RT "Identification of a gene selectively expressed in the brain, which encodes
RT a putative transmembrane protein and a soluble cytoplasmic isoform.";
RL Eur. J. Biochem. 256:24-35(1998).
CC -!- FUNCTION: Central nervous system-specific myelin protein that increase
CC myelin genes expression during oligodendrocyte differentiation.
CC Promotes oligodendrocyte terminal differentiation.
CC {ECO:0000250|UniProtKB:Q7M750}.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm
CC {ECO:0000269|PubMed:9746342}.
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane
CC {ECO:0000250|UniProtKB:Q7M750}; Single-pass type I membrane protein
CC {ECO:0000255}. Note=In the CNS, enriched in the myelin paranodal and
CC inner loop membranes, but not that of the PNS. Enriched in the leading
CC edge of extending processes. {ECO:0000250|UniProtKB:Q7M750}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q29102-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q29102-2; Sequence=VSP_003990;
CC -!- TISSUE SPECIFICITY: Specifically expressed in brain.
CC {ECO:0000269|PubMed:9746342}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X89480; CAA61669.1; -; mRNA.
DR EMBL; Y11026; CAA71910.1; -; mRNA.
DR PIR; S58082; S58082.
DR RefSeq; NP_999375.1; NM_214210.1. [Q29102-1]
DR AlphaFoldDB; Q29102; -.
DR STRING; 9823.ENSSSCP00000011194; -.
DR PaxDb; Q29102; -.
DR PRIDE; Q29102; -.
DR Ensembl; ENSSSCT00005074446; ENSSSCP00005046767; ENSSSCG00005045976. [Q29102-1]
DR GeneID; 397416; -.
DR KEGG; ssc:397416; -.
DR CTD; 93377; -.
DR eggNOG; ENOG502RWAT; Eukaryota.
DR HOGENOM; CLU_1824653_0_0_1; -.
DR InParanoid; Q29102; -.
DR TreeFam; TF337818; -.
DR Proteomes; UP000008227; Unplaced.
DR Proteomes; UP000314985; Unplaced.
DR Genevisible; Q29102; SS.
DR GO; GO:0044291; C:cell-cell contact zone; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0048713; P:regulation of oligodendrocyte differentiation; ISS:UniProtKB.
DR InterPro; IPR026609; Opalin.
DR PANTHER; PTHR21102; PTHR21102; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Cytoplasm; Glycoprotein; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..142
FT /note="Opalin"
FT /id="PRO_0000072593"
FT TOPO_DOM 1..33
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 55..142
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 78..94
FT /note="Required for plasma membrane localization"
FT /evidence="ECO:0000250|UniProtKB:Q7M750"
FT CARBOHYD 6
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 12
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 14
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..96
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_003990"
SQ SEQUENCE 142 AA; 15771 MW; FEF979931F237B8D CRC64;
MSFSLNFTLP ANTTSSPVVT SGKGADCGPS LGLAAGIPSL VATALLVALL LILIHRRRRS
SESTEEIERP CEISEIYDNP RVAENPRRSP THEKNIMGAE EAHIYVKTVS GSQEPMRDTY
RPAVEMERRR GLWWLIPRLS LE