OPA_DROME
ID OPA_DROME Reviewed; 609 AA.
AC P39768; A0JQ21; Q960Y0; Q9VN66;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 179.
DE RecName: Full=Pair-rule protein odd-paired;
GN Name=opa; ORFNames=CG1133;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC TISSUE=Embryo;
RX PubMed=8288124; DOI=10.1101/gad.8.1.105;
RA Benedyk M.J., Mullen J.R., Dinardo S.;
RT "Odd-paired: a zinc finger pair-rule protein required for the timely
RT activation of engrailed and wingless in Drosophila embryos.";
RL Genes Dev. 8:105-117(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=7781900; DOI=10.1006/dbio.1995.1171;
RA Cimbora D.M., Sakonju S.;
RT "Drosophila midgut morphogenesis requires the function of the segmentation
RT gene odd-paired.";
RL Dev. Biol. 169:580-595(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcription factor essential for parasegmental subdivision
CC of the embryo. It is involved in the activation of wingless (wg) in odd
CC parasegments. It is also required for the timely activation of wg in
CC the remaining parasegments and for the timely activation of engrailed
CC (en) in all parasegments. {ECO:0000269|PubMed:7781900,
CC ECO:0000269|PubMed:8288124}.
CC -!- INTERACTION:
CC P39768; Q9VM83: Dmel\CG17376; NbExp=4; IntAct=EBI-93813, EBI-15116127;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed throughout all segment primordia;
CC expressed ubiquitously in the ectoderm and mesoderm precursors.
CC {ECO:0000269|PubMed:7781900, ECO:0000269|PubMed:8288124}.
CC -!- DEVELOPMENTAL STAGE: The peak expression is seen between 2 and 12 hours
CC of embryogenesis. Expression continues through the larval instars and
CC during pupation although at lower levels compared with embryogenesis.
CC {ECO:0000269|PubMed:7781900, ECO:0000269|PubMed:8288124}.
CC -!- SIMILARITY: Belongs to the GLI C2H2-type zinc-finger protein family.
CC {ECO:0000305}.
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DR EMBL; U04435; AAA18958.1; -; mRNA.
DR EMBL; S78339; AAB34592.1; -; mRNA.
DR EMBL; AE014297; AAF52084.1; -; Genomic_DNA.
DR EMBL; AY051783; AAK93207.1; -; mRNA.
DR EMBL; BT029391; ABK56895.1; -; mRNA.
DR PIR; A49839; A49839.
DR RefSeq; NP_524228.2; NM_079504.4.
DR AlphaFoldDB; P39768; -.
DR SMR; P39768; -.
DR BioGRID; 65820; 36.
DR DIP; DIP-17067N; -.
DR IntAct; P39768; 25.
DR STRING; 7227.FBpp0078477; -.
DR PaxDb; P39768; -.
DR DNASU; 40605; -.
DR EnsemblMetazoa; FBtr0078836; FBpp0078477; FBgn0003002.
DR GeneID; 40605; -.
DR KEGG; dme:Dmel_CG1133; -.
DR CTD; 40605; -.
DR FlyBase; FBgn0003002; opa.
DR VEuPathDB; VectorBase:FBgn0003002; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000165216; -.
DR HOGENOM; CLU_002678_37_2_1; -.
DR InParanoid; P39768; -.
DR OMA; AHPHHAM; -.
DR OrthoDB; 592023at2759; -.
DR PhylomeDB; P39768; -.
DR SignaLink; P39768; -.
DR BioGRID-ORCS; 40605; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 40605; -.
DR PRO; PR:P39768; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0003002; Expressed in parasegment 5 (Drosophila) and 18 other tissues.
DR ExpressionAtlas; P39768; baseline and differential.
DR Genevisible; P39768; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IMP:FlyBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0007417; P:central nervous system development; IBA:GO_Central.
DR GO; GO:0007455; P:eye-antennal disc morphogenesis; IMP:FlyBase.
DR GO; GO:0008354; P:germ cell migration; IMP:FlyBase.
DR GO; GO:0007494; P:midgut development; IMP:FlyBase.
DR GO; GO:0007366; P:periodic partitioning by pair rule gene; IEA:UniProtKB-KW.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR InterPro; IPR041643; Znf_ZIC.
DR Pfam; PF00096; zf-C2H2; 2.
DR Pfam; PF18366; zf_ZIC; 1.
DR SMART; SM00355; ZnF_C2H2; 5.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; DNA-binding; Metal-binding; Nucleus;
KW Pair-rule protein; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..609
FT /note="Pair-rule protein odd-paired"
FT /id="PRO_0000047015"
FT ZN_FING 210..249
FT /note="C2H2-type 1; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 258..285
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 291..315
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 321..345
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 351..375
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 20..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 373..550
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 583..609
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 373..388
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 389..511
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 587
FT /note="P -> S (in Ref. 5; AAK93207)"
FT /evidence="ECO:0000305"
FT CONFLICT 606
FT /note="A -> R (in Ref. 1; AAA18958 and 2; AAB34592)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 609 AA; 66238 MW; 434CF5B7212FD6CD CRC64;
MMMNAFIEPA QHHLASYGLR MSPNTTASNS NAQQQQQQQL EMTQQQQQQQ QQQQQQQQQD
QESAAATAAA YQNSGYGHFN SYASRDFLLG RREAEYGVAG SAGQASAAAD SMLFSGFPAQ
AAELGSGFGQ HPFHSHHHHH QMRMGMADAY AAGHPYNHHG NFPTAAVHHP VVHHPSHHAM
SAMHPAGAGA FLRYMRHQPA SSASSVKQEM QCLWIDPDQP GLVPPGGRKT CNKVFHSMHE
IVTHLTVEHV GGPECTTHAC FWVGCSRNGR PFKAKYKLVN HIRVHTGEKP FACPHPGCGK
VFARSENLKI HKRTHTGEKP FKCEHEGCDR RFANSSDRKK HSHVHTSDKP YNCRINGCDK
SYTHPSSLRK HMKVHGNVDE KSPSHGYDSE GEESSSSSII TGGAQTPPST RLDGSAGSSS
GVSSLSGGSG IKSSPHSIKS EPNPMHSVHL GASSSGSSST ASSSASHLLQ HQQHQHQQQQ
QQQQHQQQAQ QQQQLTAHPS DPKSSPALQL MAASASAYLP PPLGPPPSHH HHPHHHQAAP
SPGAAAASAS MLHHNHHLLY HPAAQHHPPS DWYHTTAPSG SAEAMNPLNH FGHHHHHHHL
MHPGAATAY
