ID   OPCR_BACSU              Reviewed;         185 AA.
AC   O34709; Q795K5;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=HTH-type transcriptional repressor OpcR;
GN   Name=opcR; Synonyms=yvbF; OrderedLocusNames=BSU33840;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=10216873; DOI=10.1046/j.1365-2958.1999.01354.x;
RA   Kappes R.M., Kempf B., Kneip S., Boch J., Gade J., Meier-Wagner J.,
RA   Bremer E.;
RT   "Two evolutionarily closely related ABC transporters mediate the uptake of
RT   choline for synthesis of the osmoprotectant glycine betaine in Bacillus
RT   subtilis.";
RL   Mol. Microbiol. 32:203-216(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION, DNA-BINDING, ACTIVITY REGULATION, AND GENE NAME.
RC   STRAIN=168;
RX   PubMed=23960087; DOI=10.1099/mic.0.067074-0;
RA   Lee C.H., Wu T.Y., Shaw G.C.;
RT   "Involvement of OpcR, a GbsR-type transcriptional regulator, in negative
RT   regulation of two evolutionarily closely related choline uptake genes in
RT   Bacillus subtilis.";
RL   Microbiology 159:2087-2096(2013).
CC   -!- FUNCTION: Negatively regulates the transcription of the opuC operon. In
CC       the absence of GbsR, is also a negative regulator of the opuB operon.
CC       Binds to an inverted repeat in the promoter region of the operons.
CC       {ECO:0000269|PubMed:23960087}.
CC   -!- ACTIVITY REGULATION: Is not choline-responsive.
CC       {ECO:0000269|PubMed:23960087}.
CC   -!- SIMILARITY: Belongs to the GbsR family. {ECO:0000305}.
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DR   EMBL; AF009352; AAB63772.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15389.1; -; Genomic_DNA.
DR   PIR; E70029; E70029.
DR   RefSeq; NP_391264.1; NC_000964.3.
DR   RefSeq; WP_003244093.1; NZ_JNCM01000033.1.
DR   AlphaFoldDB; O34709; -.
DR   SMR; O34709; -.
DR   STRING; 224308.BSU33840; -.
DR   PaxDb; O34709; -.
DR   PRIDE; O34709; -.
DR   EnsemblBacteria; CAB15389; CAB15389; BSU_33840.
DR   GeneID; 936258; -.
DR   KEGG; bsu:BSU33840; -.
DR   PATRIC; fig|224308.179.peg.3669; -.
DR   eggNOG; COG1510; Bacteria.
DR   InParanoid; O34709; -.
DR   OMA; HAEEHLI; -.
DR   PhylomeDB; O34709; -.
DR   BioCyc; BSUB:BSU33840-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR000835; HTH_MarR-typ.
DR   InterPro; IPR026282; MJ1563.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF12802; MarR_2; 1.
DR   PIRSF; PIRSF006707; MJ1563; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..185
FT                   /note="HTH-type transcriptional repressor OpcR"
FT                   /id="PRO_0000381938"
FT   DNA_BIND        49..73
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   185 AA;  21751 MW;  2B34299E30709449 CRC64;
     MKKTALDIIE HAEEHLIEKI AENMHTFGMP STVGRVLGII YMNRKPMTLS ELSEATGMSK
     TRMSQVVREM IDANIAEKVF EKGVRKDLYD VEQDYYQTFI SLFAANWTKV VSKNKVLYKK
     LNRELSDLLQ RDGLTPEAEE KVNQLLNELK EWLHYYDWLS RLIEFFESEE VFRYVPKTKE
     CSSLK