ID   OPGD_ECO57              Reviewed;         551 AA.
AC   Q8X9V1;
DT   03-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Glucans biosynthesis protein D;
DE   Flags: Precursor;
GN   Name=mdoD; Synonyms=opgD; OrderedLocusNames=Z2294, ECs2029;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Probably involved in the control of the structural glucose
CC       backbone of osmoregulated periplasmic glucans (OPGs). {ECO:0000250}.
CC   -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250}.
CC   -!- PTM: Predicted to be exported by the Tat system. The position of the
CC       signal peptide cleavage has not been experimentally proven.
CC   -!- SIMILARITY: Belongs to the OpgD/OpgG family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG56350.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB35452.1; -; Genomic_DNA.
DR   PIR; B85736; B85736.
DR   PIR; E90882; E90882.
DR   RefSeq; NP_310056.1; NC_002695.1.
DR   RefSeq; WP_000375956.1; NZ_SEKU01000010.1.
DR   AlphaFoldDB; Q8X9V1; -.
DR   SMR; Q8X9V1; -.
DR   STRING; 155864.EDL933_2226; -.
DR   EnsemblBacteria; AAG56350; AAG56350; Z2294.
DR   EnsemblBacteria; BAB35452; BAB35452; ECs_2029.
DR   GeneID; 66674725; -.
DR   GeneID; 917232; -.
DR   KEGG; ece:Z2294; -.
DR   KEGG; ecs:ECs_2029; -.
DR   PATRIC; fig|386585.9.peg.2129; -.
DR   eggNOG; COG3131; Bacteria.
DR   HOGENOM; CLU_023403_2_0_6; -.
DR   OMA; DVQFFHV; -.
DR   UniPathway; UPA00637; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009250; P:glucan biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.60.40.10; -; 1.
DR   Gene3D; 2.70.98.10; -; 1.
DR   HAMAP; MF_01068; MdoD_OpgD; 1.
DR   InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR   InterPro; IPR014718; GH-type_carb-bd.
DR   InterPro; IPR023724; Glucan_biosyn_MdoD.
DR   InterPro; IPR014438; Glucan_biosyn_MdoG/MdoD.
DR   InterPro; IPR007444; Glucan_biosyn_MdoG_C.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR006311; TAT_signal.
DR   InterPro; IPR019546; TAT_signal_bac_arc.
DR   PANTHER; PTHR30504; PTHR30504; 1.
DR   Pfam; PF04349; MdoG; 1.
DR   PIRSF; PIRSF006281; MdoG; 1.
DR   SUPFAM; SSF74650; SSF74650; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
DR   TIGRFAMs; TIGR01409; TAT_signal_seq; 1.
DR   PROSITE; PS51318; TAT; 1.
PE   3: Inferred from homology;
KW   Periplasm; Reference proteome; Signal.
FT   SIGNAL          1..32
FT                   /note="Tat-type signal"
FT                   /evidence="ECO:0000255"
FT   CHAIN           33..551
FT                   /note="Glucans biosynthesis protein D"
FT                   /id="PRO_0000020208"
SQ   SEQUENCE   551 AA;  62768 MW;  7FC2F7072C60FA9F CRC64;
     MDRRRFIKGS MAMAAVCGTS GIASLFSQAA FAADSDIADG QTQRFDFSIL QSMAHDLAQT
     AWRGAPRPLP DTLATMTPQA YNSIQYDAEK SLWHNVENRQ LDAQFFHMGM GFRRRVRMFS
     VDPATHLARE IHFRPELFKY NDAGVDTKQL EGQSDLGFAG FRVFKAPELA RRDVVSFLGA
     SYFRAVDDTY QYGLSARGLA IDTYTDSKEE FPDFTAFWFD TVKPGATTFT VYALLDSASI
     TGAYKFTIHC EKSQVIMDVE NHLYARKDIK QLGIAPMTSM FSCGTNERRM CDTIHPQIHD
     SDRLSMWRGN GEWICRPLNN PQKLQFNAYT DNNPKGFGLL QLDRDFSHYQ DIMGWYNKRP
     SLWVEPRNKW GKGTIGLMEI PTTGETLDNI VCFWQPEKAV KAGDEFAFQY RLYWSAQPPV
     HCPLARVMAT RTGMGGFPEG WAPGEHYPEK WARRFAVDFV GGDLKAAAPK GIEPVITLSS
     GEAKQIEILY IEPIDGYRIQ FDWYPTSDST DPVDMRMYLR CQGDAISETW LYQYFPPAPD
     KRQYVDDRVM S