ID   OPGD_ECOLI              Reviewed;         551 AA.
AC   P40120; P46134; P76098; P77631;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   03-JUL-2003, sequence version 3.
DT   03-AUG-2022, entry version 153.
DE   RecName: Full=Glucans biosynthesis protein D;
DE   Flags: Precursor;
GN   Name=mdoD; Synonyms=opgD, ydcG, yzzZ; OrderedLocusNames=b1424, JW1420;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA   Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA   Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA   Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA   Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA   Wada C., Yamamoto Y., Horiuchi T.;
RT   "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 28.0-40.1 min region on the linkage map.";
RL   DNA Res. 3:363-377(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 495-551.
RC   STRAIN=MB2052;
RX   PubMed=2671655; DOI=10.1007/bf02464895;
RA   Tanaka S., Matsushita Y., Yoshikawa A., Isono K.;
RT   "Cloning and molecular characterization of the gene rimL which encodes an
RT   enzyme acetylating ribosomal protein L12 of Escherichia coli K12.";
RL   Mol. Gen. Genet. 217:289-293(1989).
RN   [5]
RP   PROTEIN SEQUENCE OF 33-44.
RC   STRAIN=K12 / EMG2;
RX   PubMed=9298646; DOI=10.1002/elps.1150180807;
RA   Link A.J., Robison K., Church G.M.;
RT   "Comparing the predicted and observed properties of proteins encoded in the
RT   genome of Escherichia coli K-12.";
RL   Electrophoresis 18:1259-1313(1997).
RN   [6]
RP   IDENTIFICATION.
RX   PubMed=7567469; DOI=10.1093/nar/23.17.3554;
RA   Borodovsky M., McIninch J., Koonin E.V., Rudd K.E., Medigue C., Danchin A.;
RT   "Detection of new genes in a bacterial genome using Markov models for three
RT   gene classes.";
RL   Nucleic Acids Res. 23:3554-3562(1995).
RN   [7]
RP   CHARACTERIZATION, AND EXPORT VIA THE TAT-SYSTEM.
RC   STRAIN=K12;
RX   PubMed=15175282; DOI=10.1128/jb.186.12.3695-3702.2004;
RA   Lequette Y., Oedberg-Ferragut C., Bohin J.-P., Lacroix J.-M.;
RT   "Identification of mdoD, an mdoG paralog which encodes a twin-arginine-
RT   dependent periplasmic protein that controls osmoregulated periplasmic
RT   glucan backbone structures.";
RL   J. Bacteriol. 186:3695-3702(2004).
RN   [8]
RP   EXPORT VIA THE TAT-SYSTEM AND THE SEC-SYSTEM.
RX   PubMed=17218314; DOI=10.1074/jbc.m610507200;
RA   Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B.,
RA   Palmer T., Georgiou G.;
RT   "Export pathway selectivity of Escherichia coli twin arginine translocation
RT   signal peptides.";
RL   J. Biol. Chem. 282:8309-8316(2007).
CC   -!- FUNCTION: Probably involved in the control of the structural glucose
CC       backbone of osmoregulated periplasmic glucans (OPGs).
CC   -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Periplasm.
CC   -!- DEVELOPMENTAL STAGE: Expressed essentially during the stationary growth
CC       phase when OPG synthesis has stopped.
CC   -!- PTM: Exported by the Tat system. The position of the signal peptide
CC       cleavage has been experimentally proven. Can also be exported by the
CC       Sec system.
CC   -!- SIMILARITY: Belongs to the OpgD/OpgG family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA15046.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U00096; AAC74506.3; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15046.1; ALT_INIT; Genomic_DNA.
DR   EMBL; X15860; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; C64894; C64894.
DR   RefSeq; NP_415941.5; NC_000913.3.
DR   RefSeq; WP_000375961.1; NZ_SSZK01000021.1.
DR   AlphaFoldDB; P40120; -.
DR   SMR; P40120; -.
DR   BioGRID; 4261154; 12.
DR   IntAct; P40120; 6.
DR   STRING; 511145.b1424; -.
DR   jPOST; P40120; -.
DR   PaxDb; P40120; -.
DR   PRIDE; P40120; -.
DR   EnsemblBacteria; AAC74506; AAC74506; b1424.
DR   EnsemblBacteria; BAA15046; BAA15046; BAA15046.
DR   GeneID; 945994; -.
DR   KEGG; ecj:JW1420; -.
DR   KEGG; eco:b1424; -.
DR   PATRIC; fig|511145.12.peg.1487; -.
DR   EchoBASE; EB2701; -.
DR   eggNOG; COG3131; Bacteria.
DR   HOGENOM; CLU_023403_2_0_6; -.
DR   InParanoid; P40120; -.
DR   OMA; DVQFFHV; -.
DR   PhylomeDB; P40120; -.
DR   BioCyc; EcoCyc:EG12859-MON; -.
DR   UniPathway; UPA00637; -.
DR   PRO; PR:P40120; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0030288; C:outer membrane-bounded periplasmic space; IDA:EcoCyc.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0051274; P:beta-glucan biosynthetic process; IBA:GO_Central.
DR   Gene3D; 2.60.40.10; -; 1.
DR   Gene3D; 2.70.98.10; -; 1.
DR   HAMAP; MF_01068; MdoD_OpgD; 1.
DR   InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR   InterPro; IPR014718; GH-type_carb-bd.
DR   InterPro; IPR023724; Glucan_biosyn_MdoD.
DR   InterPro; IPR014438; Glucan_biosyn_MdoG/MdoD.
DR   InterPro; IPR007444; Glucan_biosyn_MdoG_C.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR006311; TAT_signal.
DR   InterPro; IPR019546; TAT_signal_bac_arc.
DR   PANTHER; PTHR30504; PTHR30504; 1.
DR   Pfam; PF04349; MdoG; 1.
DR   PIRSF; PIRSF006281; MdoG; 1.
DR   SUPFAM; SSF74650; SSF74650; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
DR   TIGRFAMs; TIGR01409; TAT_signal_seq; 1.
DR   PROSITE; PS51318; TAT; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Periplasm; Reference proteome; Signal.
FT   SIGNAL          1..32
FT                   /note="Tat-type signal"
FT                   /evidence="ECO:0000269|PubMed:9298646"
FT   CHAIN           33..551
FT                   /note="Glucans biosynthesis protein D"
FT                   /id="PRO_0000020206"
SQ   SEQUENCE   551 AA;  62758 MW;  4FC3BE21F70D4C46 CRC64;
     MDRRRFIKGS MAMAAVCGTS GIASLFSQAA FAADSDIADG QTQRFDFSIL QSMAHDLAQT
     AWRGAPRPLP DTLATMTPQA YNSIQYDAEK SLWHNVENRQ LDAQFFHMGM GFRRRVRMFS
     VDPATHLARE IHFRPELFKY NDAGVDTKQL EGQSDLGFAG FRVFKAPELA RRDVVSFLGA
     SYFRAVDDTY QYGLSARGLA IDTYTDSKEE FPDFTAFWFD TVKPGATTFT VYALLDSASI
     TGAYKFTIHC EKSQVIMDVE NHLYARKDIK QLGIAPMTSM FSCGTNERRM CDTIHPQIHD
     SDRLSMWRGN GEWICRPLNN PQKLQFNAYT DNNPKGFGLL QLDRDFSHYQ DIMGWYNKRP
     SLWVEPRNKW GKGTIGLMEI PTTGETLDNI VCFWQPEKAV KAGDEFAFQY RLYWSAQPPV
     HCPLARVMAT RTGMGGFSEG WAPGEHYPEK WARRFAVDFV GGDLKAAAPK GIEPVITLSS
     GEAKQIEILY IEPIDGYRIQ FDWYPTSDST DPVDMRMYLR CQGDAISETW LYQYFPPAPD
     KRQYVDDRVM S