OPGE_ECOLI
ID OPGE_ECOLI Reviewed; 527 AA.
AC P75785;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Phosphoethanolamine transferase OpgE;
DE EC=2.7.-.-;
GN Name=opgE; Synonyms=ybiP; OrderedLocusNames=b0815, JW0800;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS], AND SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [5]
RP FUNCTION, PATHWAY, AND GENE NAME.
RC STRAIN=K12 / ATCC 35607 / JM83;
RX PubMed=24228245; DOI=10.1155/2013/371429;
RA Bontemps-Gallo S., Cogez V., Robbe-Masselot C., Quintard K., Dondeyne J.,
RA Madec E., Lacroix J.M.;
RT "Biosynthesis of osmoregulated periplasmic glucans in Escherichia coli: the
RT phosphoethanolamine transferase is encoded by opgE.";
RL Biomed. Res. Int. 2013:371429-371429(2013).
CC -!- FUNCTION: Catalyzes the addition of a phosphoethanolamine moiety to the
CC osmoregulated periplasmic glucan (OPG) backbone.
CC {ECO:0000269|PubMed:24228245}.
CC -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC biosynthesis. {ECO:0000269|PubMed:24228245}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15919996}.
CC -!- SIMILARITY: Belongs to the phosphoethanolamine transferase family.
CC {ECO:0000305}.
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DR EMBL; U00096; AAC73902.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35487.1; -; Genomic_DNA.
DR PIR; G64818; G64818.
DR RefSeq; NP_415336.1; NC_000913.3.
DR RefSeq; WP_001054678.1; NZ_STEB01000019.1.
DR AlphaFoldDB; P75785; -.
DR SMR; P75785; -.
DR BioGRID; 4259975; 3.
DR IntAct; P75785; 3.
DR STRING; 511145.b0815; -.
DR jPOST; P75785; -.
DR PaxDb; P75785; -.
DR PRIDE; P75785; -.
DR EnsemblBacteria; AAC73902; AAC73902; b0815.
DR EnsemblBacteria; BAA35487; BAA35487; BAA35487.
DR GeneID; 945360; -.
DR KEGG; ecj:JW0800; -.
DR KEGG; eco:b0815; -.
DR PATRIC; fig|1411691.4.peg.1463; -.
DR EchoBASE; EB3105; -.
DR eggNOG; COG2194; Bacteria.
DR HOGENOM; CLU_039390_3_0_6; -.
DR OMA; EVQYLAH; -.
DR PhylomeDB; P75785; -.
DR BioCyc; EcoCyc:G6418-MON; -.
DR BioCyc; MetaCyc:G6418-MON; -.
DR BRENDA; 2.7.8.43; 2026.
DR UniPathway; UPA00637; -.
DR PRO; PR:P75785; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; ISM:EcoCyc.
DR GO; GO:0016776; F:phosphotransferase activity, phosphate group as acceptor; IBA:GO_Central.
DR GO; GO:0008484; F:sulfuric ester hydrolase activity; IEA:InterPro.
DR GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IBA:GO_Central.
DR GO; GO:1900727; P:osmoregulated periplasmic glucan biosynthetic process; IMP:EcoCyc.
DR Gene3D; 3.40.720.10; -; 1.
DR InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR InterPro; IPR040423; PEA_transferase.
DR InterPro; IPR000917; Sulfatase_N.
DR PANTHER; PTHR30443; PTHR30443; 1.
DR Pfam; PF00884; Sulfatase; 1.
DR SUPFAM; SSF53649; SSF53649; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..527
FT /note="Phosphoethanolamine transferase OpgE"
FT /id="PRO_0000209145"
FT TOPO_DOM 1..33
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 55..62
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 84..106
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 128..146
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 168..527
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 527 AA; 59706 MW; 7069D6C1A5503A2A CRC64;
MNLTLKESLV TRSRVFSPWT AFYFLQSLLI NLGLGYPFSL LYTAAFTAIL LLLWRTLPRV
QKVLVGVSSL VAACYFPFAQ AYGAPNFNTL LALHSTNMEE STEILTIFPW YSYLVGLFIF
ALGVIAIRRK KENEKARWNT FDSLCLVFSV ATFFVAPVQN LAWGGVFKLK DTGYPVFRFA
KDVIVNNNEV IEEQERMAKL SGMKDTWTVT AVKPKYQTYV VVIGESARRD ALGAFGGHWD
NTPFASSVNG LIFADYIAAS GSTQKSLGLT LNRVVDGKPQ FQDNFVTLAN RAGFQTWWFS
NQGQIGEYDT AIASIAKRAD EVYFLKEGNF EADKNTKDEA LLDMTAQVLA QEHSQPQLIV
LHLMGSHPQA CDRTQGKYET FVQSKETSCY LYTMTQTDDL LRKLYDQLRN SGSSFSLVYF
SDHGLAFKER GKDVQYLAHD DKYQQNFQVP FMVISSDDKA HRVIKARRSA NDFLGFFSQW
TGIKAKEINI KYPFISEKKA GPIYITNFQL QKVDYNHLGT DIFDPKP
