ID   ARY1_CALVI              Reviewed;         759 AA.
AC   P28513; Q23815;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Arylphorin subunit A4;
DE   Flags: Precursor;
OS   Calliphora vicina (Blue blowfly) (Calliphora erythrocephala).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Oestroidea;
OC   Calliphoridae; Calliphorinae; Calliphora.
OX   NCBI_TaxID=7373;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Fat body;
RX   PubMed=1711849; DOI=10.1016/0006-291x(91)90632-h;
RA   Naumann U., Scheller K.;
RT   "Complete cDNA and gene sequence of the developmentally regulated
RT   arylphorin of Calliphora vicina and its homology to insect hemolymph
RT   proteins and arthropod hemocyanins.";
RL   Biochem. Biophys. Res. Commun. 177:963-972(1991).
CC   -!- FUNCTION: Arylphorin is a larval storage protein (LSP) which may serve
CC       as a storage protein used primarily as a source of aromatic amino acids
CC       for protein synthesis during metamorphosis. It is a constituent of the
CC       sclerotizing system of the cuticle, and serves as a carrier for
CC       ecdysteroid hormone.
CC   -!- SUBUNIT: Heterohexamer.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space.
CC   -!- TISSUE SPECIFICITY: Fat body.
CC   -!- SIMILARITY: Belongs to the hemocyanin family. {ECO:0000305}.
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DR   EMBL; M76480; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; X59391; CAA42034.1; -; mRNA.
DR   PIR; JQ1045; JQ1045.
DR   AlphaFoldDB; P28513; -.
DR   SMR; P28513; -.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1280.10; -; 1.
DR   Gene3D; 1.20.1370.10; -; 1.
DR   Gene3D; 2.60.40.1520; -; 1.
DR   InterPro; IPR008922; Di-copper_centre_dom_sf.
DR   InterPro; IPR013788; Hemocyanin/hexamerin.
DR   InterPro; IPR000896; Hemocyanin/hexamerin_mid_dom.
DR   InterPro; IPR005203; Hemocyanin_C.
DR   InterPro; IPR037020; Hemocyanin_C_sf.
DR   InterPro; IPR005204; Hemocyanin_N.
DR   InterPro; IPR036697; Hemocyanin_N_sf.
DR   InterPro; IPR014756; Ig_E-set.
DR   PANTHER; PTHR11511; PTHR11511; 1.
DR   Pfam; PF03723; Hemocyanin_C; 1.
DR   Pfam; PF00372; Hemocyanin_M; 2.
DR   Pfam; PF03722; Hemocyanin_N; 2.
DR   PRINTS; PR00187; HAEMOCYANIN.
DR   SUPFAM; SSF48050; SSF48050; 1.
DR   SUPFAM; SSF48056; SSF48056; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
DR   PROSITE; PS00210; HEMOCYANIN_2; 1.
PE   2: Evidence at transcript level;
KW   Secreted; Signal; Storage protein.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255"
FT   CHAIN           17..759
FT                   /note="Arylphorin subunit A4"
FT                   /id="PRO_0000013331"
FT   CONFLICT        231
FT                   /note="N -> D (in Ref. 1; CAA42034)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        351
FT                   /note="N -> H (in Ref. 1; CAA42034)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        607
FT                   /note="D -> E (in Ref. 1; CAA42034)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        683
FT                   /note="G -> E (in Ref. 1; CAA42034)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   759 AA;  92342 MW;  3D222108A38B4BBD CRC64;
     MKIAIVLLAI IALVAASSIS KHEVKIADKE FLAKQKFLFE IVYRVEDPLM FEEWIKMGKT
     FTFDKSGYTH FDMYMEKFYE AYKYGAILPK GEFFYYAKNW ETFQRNVAFA RMHFNEGMFV
     YALTLAVIHR DDFQGLILPS IHEIFPQYFF NSKFVYEAEK FDYDVWSKYI MYEKEYKDIL
     YKDYSTFYKN HDNHHYYYFT KDFKTYQWWK MMGLGEHWYS EDRFMLRDNM NKYNKDSKYL
     EIFEGTKMFF MPVDYTRDIE FFNKESVLSY FTEDVGLNTY WYYLNMDYAF ILDGKTFGLN
     KDRRGEYWLY NVRQLLSRYY MERLTHGYGE IPHFSFLNTI EHGYDSQLVY NNGVGFSYRK
     NYYEVESYGK FSYYYKVMDF FNRLDEIITK GVYVTYEGKT IDLRKPESIE YIGSIMQGNV
     DTFDNYFFKY RYMFAHMYFG DVNTHDFEVF PHIFLNYETM MRDPMFYMFY KKIASVYFQF
     FNYVKPYTHE ELLFPGVTIK DVKVSELVTY FDLVDFDVTN LMNDEMTFVD GQFVWDKTLL
     ARQMRLNHKP FDFDFVIESD KSHKVVIRTF LGPKYDEFGR VITLTENRQN FMEIDSFIYT
     LKSGVNDFKR LSKDFYWTVE DRTTYNELYK YVMLALQGKY DFPLDISEPH CGFPDRLVLP
     HGWYKGMPMQ FFFYIAPYTA SYGPFSTYDS TYACGIGSGV RHIDEMPFGY PFDREIDEYE
     FFVPNMYFKD VKIYHQDTFD KYYGKKYENF GHFDYSYYH