ID   ARY1_DICDI              Reviewed;         325 AA.
AC   Q54IU9;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Probable arylamine N-acetyltransferase 1;
DE            EC=2.3.1.5;
DE   AltName: Full=Arylamide acetylase 1;
DE   AltName: Full=N-acetyltransferase type 1;
DE            Short=NAT-1;
GN   ORFNames=DDB_G0288503;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + an arylamine = an N-acetylarylamine + CoA;
CC         Xref=Rhea:RHEA:16613, ChEBI:CHEBI:13790, ChEBI:CHEBI:50471,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.5;
CC   -!- SIMILARITY: Belongs to the arylamine N-acetyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000112; EAL63200.1; -; Genomic_DNA.
DR   RefSeq; XP_636704.1; XM_631612.1.
DR   AlphaFoldDB; Q54IU9; -.
DR   SMR; Q54IU9; -.
DR   PaxDb; Q54IU9; -.
DR   EnsemblProtists; EAL63200; EAL63200; DDB_G0288503.
DR   GeneID; 8626661; -.
DR   KEGG; ddi:DDB_G0288503; -.
DR   dictyBase; DDB_G0288503; nat2.
DR   eggNOG; ENOG502RI6E; Eukaryota.
DR   HOGENOM; CLU_049918_4_0_1; -.
DR   InParanoid; Q54IU9; -.
DR   PhylomeDB; Q54IU9; -.
DR   PRO; PR:Q54IU9; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0004060; F:arylamine N-acetyltransferase activity; IBA:GO_Central.
DR   InterPro; IPR001447; Arylamine_N-AcTrfase.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   PANTHER; PTHR11786; PTHR11786; 1.
DR   Pfam; PF00797; Acetyltransf_2; 1.
DR   SUPFAM; SSF54001; SSF54001; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..325
FT                   /note="Probable arylamine N-acetyltransferase 1"
FT                   /id="PRO_0000327944"
FT   ACT_SITE        72
FT                   /note="Acyl-thioester intermediate"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        112
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        127
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   325 AA;  37294 MW;  C79C23FFE8B92210 CRC64;
     MNPFTDYQVQ FFERIKLKPR IIESLDDISE VLVACSKVFT FENLDIISNT QEALTRKVLI
     KQVLINKQGG LCYKANTLLY YFLLDFGLNV HQTRATCENQ ESHSRWNIGH GHMINILNFE
     NKLYVMDVAF GCNLSLRPVP ITDDGSEVIE SCIGLYRVIK RENIVAGVYH YTHILEHRKP
     DTYLIESAKN WVIGYAFDPL LICKNDGDDD DDDNNNNNNT HQTQIQQLVI DDPNKDFCVK
     PLATKLINNT DNNNNNNSIA TLTLNSFTLT NCKTGEKIKT NFDNDNLFEQ FNQHLISIFN
     LPPLKIIPQI FLNQTSNFPI NNLNS