ID   ARY1_FELCA              Reviewed;         258 AA.
AC   O62696;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Arylamine N-acetyltransferase 1;
DE            EC=2.3.1.5;
DE   AltName: Full=Arylamide acetylase 1;
DE   AltName: Full=N-acetyltransferase type 1;
DE            Short=NAT-1;
DE   Flags: Fragment;
GN   Name=NAT1; Synonyms=NAT;
OS   Felis catus (Cat) (Felis silvestris catus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Carnivora; Feliformia; Felidae; Felinae; Felis.
OX   NCBI_TaxID=9685;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10022754; DOI=10.1097/00008571-199804000-00009;
RA   Trepanier L.A., Cribb A.E., Spielberg S.P., Ray K.;
RT   "Deficiency of cytosolic arylamine N-acetylation in the domestic cat and
RT   wild felids caused by the presence of a single NAT1-like gene.";
RL   Pharmacogenetics 8:169-179(1998).
CC   -!- FUNCTION: Participates in the detoxification of a plethora of hydrazine
CC       and arylamine drugs.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + an arylamine = an N-acetylarylamine + CoA;
CC         Xref=Rhea:RHEA:16613, ChEBI:CHEBI:13790, ChEBI:CHEBI:50471,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.5;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the arylamine N-acetyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AF030398; AAC18940.1; -; Genomic_DNA.
DR   AlphaFoldDB; O62696; -.
DR   SMR; O62696; -.
DR   InParanoid; O62696; -.
DR   Proteomes; UP000011712; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004060; F:arylamine N-acetyltransferase activity; IBA:GO_Central.
DR   InterPro; IPR001447; Arylamine_N-AcTrfase.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   PANTHER; PTHR11786; PTHR11786; 1.
DR   Pfam; PF00797; Acetyltransf_2; 1.
DR   PRINTS; PR01543; ANATRNSFRASE.
DR   SUPFAM; SSF54001; SSF54001; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Cytoplasm; Reference proteome; Transferase.
FT   CHAIN           <1..>258
FT                   /note="Arylamine N-acetyltransferase 1"
FT                   /id="PRO_0000107903"
FT   ACT_SITE        59
FT                   /note="Acyl-thioester intermediate"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        98
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        113
FT                   /evidence="ECO:0000250"
FT   BINDING         97..98
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         199
FT                   /ligand="CoA"
FT                   /ligand_id="ChEBI:CHEBI:57287"
FT                   /evidence="ECO:0000250"
FT   BINDING         205
FT                   /ligand="CoA"
FT                   /ligand_id="ChEBI:CHEBI:57287"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
FT   NON_TER         258
SQ   SEQUENCE   258 AA;  30138 MW;  AD31B8745E79F2C3 CRC64;
     IGYKNARNKL DLETLTDILQ HQIRAIPFEN LNIHCGETME LGLKAIFEQV VWRNRGGWCL
     QVNHLLYWVL TTIGYETTML GGYVYSTAAN KYSNAMIHLL LKVTTEGKNY IVDAGFGRSY
     QMWQPLELIS GKDQLQVPCI FRLTEERGIW YLDQIRRQQY IANEEFLNSD LLEKNKYRKI
     YSFTLEPRTI EDFESVNTYL QTSPTSVFTS KSFCSLQTSE GVHCLVGCTL TYRKFNYKDN
     MDLVEFKVLN EEEVEQKL