OPGH_DICD3
ID OPGH_DICD3 Reviewed; 850 AA.
AC Q9F495; E0SHS0;
DT 25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Glucans biosynthesis glucosyltransferase H {ECO:0000255|HAMAP-Rule:MF_01072};
DE EC=2.4.1.- {ECO:0000255|HAMAP-Rule:MF_01072};
GN Name=opgH {ECO:0000255|HAMAP-Rule:MF_01072};
GN OrderedLocusNames=Dda3937_03563;
OS Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Pectobacteriaceae; Dickeya.
OX NCBI_TaxID=198628;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=3937;
RX PubMed=11325942; DOI=10.1128/jb.183.10.3134-3141.2001;
RA Page F., Altabe S., Hugouvieux-Cotte-Pattat N., Lacroix J.-M.,
RA Robert-Baudouy J., Bohin J.-P.;
RT "Osmoregulated periplasmic glucan synthesis is required for Erwinia
RT chrysanthemi pathogenicity.";
RL J. Bacteriol. 183:3134-3141(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3937;
RX PubMed=21217001; DOI=10.1128/jb.01513-10;
RA Glasner J.D., Yang C.H., Reverchon S., Hugouvieux-Cotte-Pattat N.,
RA Condemine G., Bohin J.P., Van Gijsegem F., Yang S., Franza T., Expert D.,
RA Plunkett G. III, San Francisco M.J., Charkowski A.O., Py B., Bell K.,
RA Rauscher L., Rodriguez-Palenzuela P., Toussaint A., Holeva M.C., He S.Y.,
RA Douet V., Boccara M., Blanco C., Toth I., Anderson B.D., Biehl B.S.,
RA Mau B., Flynn S.M., Barras F., Lindeberg M., Birch P.R., Tsuyumu S.,
RA Shi X., Hibbing M., Yap M.N., Carpentier M., Dassa E., Umehara M.,
RA Kim J.F., Rusch M., Soni P., Mayhew G.F., Fouts D.E., Gill S.R.,
RA Blattner F.R., Keen N.T., Perna N.T.;
RT "Genome sequence of the plant-pathogenic bacterium Dickeya dadantii 3937.";
RL J. Bacteriol. 193:2076-2077(2011).
CC -!- FUNCTION: Involved in the biosynthesis of osmoregulated periplasmic
CC glucans (OPGs). Seems to be required for pathogenicity.
CC {ECO:0000255|HAMAP-Rule:MF_01072, ECO:0000269|PubMed:11325942}.
CC -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01072}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01072}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01072}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. OpgH
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01072}.
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DR EMBL; AJ294718; CAC13111.1; -; Genomic_DNA.
DR EMBL; CP002038; ADM99013.1; -; Genomic_DNA.
DR RefSeq; WP_013318454.1; NC_014500.1.
DR AlphaFoldDB; Q9F495; -.
DR STRING; 198628.Dda3937_03563; -.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR EnsemblBacteria; ADM99013; ADM99013; Dda3937_03563.
DR GeneID; 9734253; -.
DR KEGG; ddd:Dda3937_03563; -.
DR PATRIC; fig|198628.6.peg.2806; -.
DR eggNOG; COG2943; Bacteria.
DR HOGENOM; CLU_015730_1_0_6; -.
DR OMA; TAGLHYW; -.
DR OrthoDB; 1049243at2; -.
DR BioCyc; DDAD198628:DDA3937_RS13215-MON; -.
DR UniPathway; UPA00637; -.
DR Proteomes; UP000006859; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009250; P:glucan biosynthetic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.90.550.10; -; 1.
DR HAMAP; MF_01072; MdoH_OpgH; 1.
DR InterPro; IPR023725; Glucans_biosynth_gluTrFase_H.
DR InterPro; IPR001173; Glyco_trans_2-like.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF00535; Glycos_transf_2; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Glycosyltransferase; Membrane;
KW Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..850
FT /note="Glucans biosynthesis glucosyltransferase H"
FT /id="PRO_0000210352"
FT TRANSMEM 148..165
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT TRANSMEM 203..225
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT TRANSMEM 521..543
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT TRANSMEM 577..599
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT TRANSMEM 612..634
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT TRANSMEM 689..711
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT CONFLICT 443
FT /note="P -> A (in Ref. 1; CAC13111)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 850 AA; 96800 MW; 64B4113F91775DAF CRC64;
MNKSTSTLEY IEKLPLPAEQ AEALREKFSA SQDLSSLHQA LSDGSVVTMQ SPDDIPLVSV
PRRLELAWED GLNGGKQLGK DREGRTALQA MPRITRASMF PDAWQTNPMV RWWDMINGRS
KPLRHHDKTA EENEAENRWR RVGTLRRYVL LLLTLFQTAI ATWYMKTILP YQGWALIDPF
AMVHQDVWRT IMQLLPYVLQ SGILILFAIL FCWVSAGFWT ALMGFLQLLI GKDKYSISST
TTGNEPLNPE HRTALIMPIC NEDVERVFAG LRATYESVEA TGELDHFDIY VLSDSNDPDI
CVAEQKAWMD LCREVGGAGR IFYRRRRRRV KRKSGNIDDF CRRWGNQYSY MVVLDADSVM
SGECLTSLVR LMDANPRAGI IQSAPRASGM DTLYARCQQF ATRVYGPLFT AGLHFWQLGE
SHYWGHNAII RVKPFIEHCA LAPLPGEGSF AGSILSHDFV EAALMRRAGW GVWIAYDLPG
SYEELPPNLL DELKRDRRWC HGNLMNFRLF LVKGMHPVHR AVFLTGVMSY LSAPLWFMFL
ALSTALQVVH TLMEPQYFLQ PRQLFPVWPQ WRPELAIALF STTLVLLFLP KLLSVILVCA
KGAKSYGGVA KLFISLLVEM LFSVLLAPVR MLFHTVFVVS AFLGWSVQWK SPQRDDDATP
WSEAFARHGS QLLLGLVWAG GMAWLDLRFL WWLAPIVFSL ILSPLVSVLS SRAGLGLACK
RAKLLLIPEE SDPPRELVAT DEYFRRNRER KLDHGFMHAI FDPSINALTS AMATARHRFS
RPIEDMREQR VNDALSRKPQ DVDCNLRLAL MSDPVTLARL HHRVWSQPDT HSDWRSHYQT
LAAPVIKSAQ
