OPGH_ECOL6
ID OPGH_ECOL6 Reviewed; 847 AA.
AC Q8FIS3;
DT 25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2003, sequence version 2.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=Glucans biosynthesis glucosyltransferase H {ECO:0000255|HAMAP-Rule:MF_01072};
DE EC=2.4.1.- {ECO:0000255|HAMAP-Rule:MF_01072};
GN Name=mdoH {ECO:0000255|HAMAP-Rule:MF_01072};
GN Synonyms=opgH {ECO:0000255|HAMAP-Rule:MF_01072}; OrderedLocusNames=c1314;
OS Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=199310;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CFT073 / ATCC 700928 / UPEC;
RX PubMed=12471157; DOI=10.1073/pnas.252529799;
RA Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA Donnenberg M.S., Blattner F.R.;
RT "Extensive mosaic structure revealed by the complete genome sequence of
RT uropathogenic Escherichia coli.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC -!- FUNCTION: Involved in the biosynthesis of osmoregulated periplasmic
CC glucans (OPGs). {ECO:0000255|HAMAP-Rule:MF_01072}.
CC -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01072}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01072}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01072}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. OpgH
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01072}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN79787.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE014075; AAN79787.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_001304473.1; NC_004431.1.
DR AlphaFoldDB; Q8FIS3; -.
DR STRING; 199310.c1314; -.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR EnsemblBacteria; AAN79787; AAN79787; c1314.
DR KEGG; ecc:c1314; -.
DR eggNOG; COG2943; Bacteria.
DR HOGENOM; CLU_015730_0_0_6; -.
DR OMA; TAGLHYW; -.
DR UniPathway; UPA00637; -.
DR Proteomes; UP000001410; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009250; P:glucan biosynthetic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.90.550.10; -; 1.
DR HAMAP; MF_01072; MdoH_OpgH; 1.
DR InterPro; IPR023725; Glucans_biosynth_gluTrFase_H.
DR InterPro; IPR001173; Glyco_trans_2-like.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF00535; Glycos_transf_2; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Glycosyltransferase; Membrane;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..847
FT /note="Glucans biosynthesis glucosyltransferase H"
FT /id="PRO_0000210351"
FT TOPO_DOM 1..138
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..156
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT TOPO_DOM 157..193
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..216
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT TOPO_DOM 217..511
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 512..534
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT TOPO_DOM 535..567
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 568..590
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT TOPO_DOM 591..602
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 603..625
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT TOPO_DOM 626..679
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 680..702
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT TOPO_DOM 703..847
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 847 AA; 96923 MW; A90636DFCC428ADD CRC64;
MNKTTEYIDA MPIAASEKAA LPKTDIRAVH QALDAEHRTW AREDDSPQGS VKARLEQAWP
DSLADGQLIK DDEGRDQLKA MPEAKRSSMF PDPWRTNPVG RFWDRLRGRD VTPRYLARLT
KEEQESEQKW RTVGTIRRYI LLILTLAQTV VATWYMKTIL PYQGWALINP MDMVGQDVWV
SFMQLLPYML QTGILILFAV LFCWVSAGFW TALMGFLQLL IGRDKYSISA STVGDEPLNP
EHRTALIMPI CNEDVNRVFA GLRATWESVK ATGNAKHFDV YILSDSYNPD ICVAEQKAWM
ELIAEVGGEG QIFYRRRRRR VKRKSGNIDD FCRRWGSQYS YMVVLDADSV MTGDCLCGLV
RLMEANPNAG IIQSSPKASG MDTLYARCQQ FATRVYGPLF TAGLHFWQLG ESHYWGHNAI
IRVKPFIEHC ALAPLPGEGS FAGSILSHDF VEAALMRRAG WGVWIAYDLP GSYEELPPNL
LDELKRDRRW CHGNLMNFRL FLVKGMHPVH RAVFLTGVMS YLSAPLWFMF LALSTALQVV
HALTEPQYFL QPRQLFPVWP QWRPELAIAL FASTMVLLFL PKLLSILLIW CKGTKEYGGF
WRVTLSLLLE VLFSVLLAPV RMLFHTVFVV SAFLGWEVVW NSPQRDDDST SWGEAFKRHG
SQLLLGLVWA VGMAWLDLRF LFWLAPIVFS LILSPFVSVI SSRATVGLRT KRWKLFLIPE
EYSPPQVLVD TDRFLEMNRQ RSLDDGFMHA VFNPSFNALA TAMATARHRA SKVLEIARDR
HVEQALNETP EKLNRDRRLV LLSDPVTMAR LHFRVWNSPE RYSSWVSYYE GIKLNPLALR
KPDAASQ
