OPGH_ECOLI
ID OPGH_ECOLI Reviewed; 847 AA.
AC P62517; P33137; P77371;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Glucans biosynthesis glucosyltransferase H;
DE EC=2.4.1.-;
GN Name=mdoH; Synonyms=opgH; OrderedLocusNames=b1049, JW1037;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=7934824; DOI=10.1111/j.1365-2958.1993.tb01959.x;
RA Loubens I., Debarbieux L., Bohin A., Lacroix J.-M., Bohin J.-P.;
RT "Homology between a genetic locus (mdoA) involved in the osmoregulated
RT biosynthesis of periplasmic glucans in Escherichia coli and a genetic locus
RT (hrpM) controlling pathogenicity of Pseudomonas syringae.";
RL Mol. Microbiol. 10:329-340(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP TOPOLOGY.
RC STRAIN=K12;
RX PubMed=9352918; DOI=10.1128/jb.179.21.6692-6698.1997;
RA Debarbieux L., Bohin A., Bohin J.-P.;
RT "Topological analysis of the membrane-bound glucosyltransferase, MdoH,
RT required for osmoregulated periplasmic glucan synthesis in Escherichia
RT coli.";
RL J. Bacteriol. 179:6692-6698(1997).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Involved in the biosynthesis of osmoregulated periplasmic
CC glucans (OPGs).
CC -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. OpgH
CC subfamily. {ECO:0000305}.
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DR EMBL; X64197; CAA45522.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74133.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35848.1; -; Genomic_DNA.
DR PIR; F64847; F64847.
DR RefSeq; NP_415567.1; NC_000913.3.
DR RefSeq; WP_001295445.1; NZ_STEB01000016.1.
DR AlphaFoldDB; P62517; -.
DR BioGRID; 4260685; 160.
DR IntAct; P62517; 1.
DR STRING; 511145.b1049; -.
DR TCDB; 4.D.3.1.1; the glycan glucosyl transferase (opgh) family.
DR jPOST; P62517; -.
DR PaxDb; P62517; -.
DR PRIDE; P62517; -.
DR EnsemblBacteria; AAC74133; AAC74133; b1049.
DR EnsemblBacteria; BAA35848; BAA35848; BAA35848.
DR GeneID; 66670683; -.
DR GeneID; 945624; -.
DR KEGG; ecj:JW1037; -.
DR KEGG; eco:b1049; -.
DR PATRIC; fig|511145.12.peg.1091; -.
DR EchoBASE; EB1832; -.
DR eggNOG; COG2943; Bacteria.
DR HOGENOM; CLU_015730_1_0_6; -.
DR InParanoid; P62517; -.
DR OMA; TAGLHYW; -.
DR PhylomeDB; P62517; -.
DR BioCyc; EcoCyc:EG11886-MON; -.
DR UniPathway; UPA00637; -.
DR PRO; PR:P62517; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0016758; F:hexosyltransferase activity; IBA:GO_Central.
DR GO; GO:1900727; P:osmoregulated periplasmic glucan biosynthetic process; IDA:EcoCyc.
DR GO; GO:0008361; P:regulation of cell size; IMP:EcoCyc.
DR Gene3D; 3.90.550.10; -; 1.
DR HAMAP; MF_01072; MdoH_OpgH; 1.
DR InterPro; IPR023725; Glucans_biosynth_gluTrFase_H.
DR InterPro; IPR001173; Glyco_trans_2-like.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF00535; Glycos_transf_2; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Glycosyltransferase; Membrane;
KW Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..847
FT /note="Glucans biosynthesis glucosyltransferase H"
FT /id="PRO_0000210349"
FT TOPO_DOM 1..139
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 161..193
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 215..512
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 513..533
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 534..569
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 570..590
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 591..602
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 603..625
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 626..679
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 680..700
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 701..847
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 19
FT /note="A -> G (in Ref. 1; CAA45522)"
FT /evidence="ECO:0000305"
FT CONFLICT 289
FT /note="P -> L (in Ref. 1; CAA45522)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 847 AA; 96937 MW; 7DCAF93640180944 CRC64;
MNKTTEYIDA MPIAASEKAA LPKTDIRAVH QALDAEHRTW AREDDSPQGS VKARLEQAWP
DSLADGQLIK DDEGRDQLKA MPEAKRSSMF PDPWRTNPVG RFWDRLRGRD VTPRYLARLT
KEEQESEQKW RTVGTIRRYI LLILTLAQTV VATWYMKTIL PYQGWALINP MDMVGQDLWV
SFMQLLPYML QTGILILFAV LFCWVSAGFW TALMGFLQLL IGRDKYSISA STVGDEPLNP
EHRTALIMPI CNEDVNRVFA GLRATWESVK ATGNAKHFDV YILSDSYNPD ICVAEQKAWM
ELIAEVGGEG QIFYRRRRRR VKRKSGNIDD FCRRWGSQYS YMVVLDADSV MTGDCLCGLV
RLMEANPNAG IIQSSPKASG MDTLYARCQQ FATRVYGPLF TAGLHFWQLG ESHYWGHNAI
IRVKPFIEHC ALAPLPGEGS FAGSILSHDF VEAALMRRAG WGVWIAYDLP GSYEELPPNL
LDELKRDRRW CHGNLMNFRL FLVKGMHPVH RAVFLTGVMS YLSAPLWFMF LALSTALQVV
HALTEPQYFL QPRQLFPVWP QWRPELAIAL FASTMVLLFL PKLLSILLIW CKGTKEYGGF
WRVTLSLLLE VLFSVLLAPV RMLFHTVFVV SAFLGWEVVW NSPQRDDDST SWGEAFKRHG
SQLLLGLVWA VGMAWLDLRF LFWLAPIVFS LILSPFVSVI SSRATVGLRT KRWKLFLIPE
EYSPPQVLVD TDRFLEMNRQ RSLDDGFMHA VFNPSFNALA TAMATARHRA SKVLEIARDR
HVEQALNETP EKLNRDRRLV LLSDPVTMAR LHFRVWNSPE RYSSWVSYYE GIKLNPLALR
KPDAASQ
