ID   OPGH_PSEA8              Reviewed;         861 AA.
AC   B7V3H0;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Glucans biosynthesis glucosyltransferase H {ECO:0000255|HAMAP-Rule:MF_01072};
DE            EC=2.4.1.- {ECO:0000255|HAMAP-Rule:MF_01072};
GN   Name=opgH {ECO:0000255|HAMAP-Rule:MF_01072}; OrderedLocusNames=PLES_54671;
OS   Pseudomonas aeruginosa (strain LESB58).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=557722;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LESB58;
RX   PubMed=19047519; DOI=10.1101/gr.086082.108;
RA   Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I.,
RA   Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A.,
RA   Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D.,
RA   Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.;
RT   "Newly introduced genomic prophage islands are critical determinants of in
RT   vivo competitiveness in the Liverpool epidemic strain of Pseudomonas
RT   aeruginosa.";
RL   Genome Res. 19:12-23(2009).
CC   -!- FUNCTION: Involved in the biosynthesis of osmoregulated periplasmic
CC       glucans (OPGs). {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01072}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01072}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. OpgH
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01072}.
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DR   EMBL; FM209186; CAW30221.1; -; Genomic_DNA.
DR   RefSeq; WP_003103448.1; NC_011770.1.
DR   AlphaFoldDB; B7V3H0; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   PRIDE; B7V3H0; -.
DR   KEGG; pag:PLES_54671; -.
DR   HOGENOM; CLU_015730_0_0_6; -.
DR   OMA; TAGLHYW; -.
DR   UniPathway; UPA00637; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009250; P:glucan biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.550.10; -; 1.
DR   HAMAP; MF_01072; MdoH_OpgH; 1.
DR   InterPro; IPR023725; Glucans_biosynth_gluTrFase_H.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Glycosyltransferase; Membrane;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..861
FT                   /note="Glucans biosynthesis glucosyltransferase H"
FT                   /id="PRO_1000136657"
FT   TRANSMEM        142..162
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        188..208
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        516..536
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        573..593
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        600..620
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        683..703
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
SQ   SEQUENCE   861 AA;  96995 MW;  63557B5A83107E7E CRC64;
     MNNPSTTKAP LADYLAHLPL AEEERERLGE SASFSELHAR LAGAEGAAAD AGGDPALASV
     RARLQLGTPE LDDAEMFGVD AQGRTFLKIS PPIRRTKVIP EPWRTNILVR GWRRLTGRSN
     PPKPKRALPR ARWQRVGSLR RFILLLLMLA QTSVATYYMK GILPYQGWAF VDLEELAQQS
     LLDTVQQVLP YVIQFGILAL FAILFCWVSA GFWTALMGFW ELLTGRDRYR ISGSSAGSEP
     IAADARTAIV MPICNEDVPR VFAGLRATVE SMAATGEMER FDFFVLSDTN DPDIAVAEQQ
     AWLELCRETK GFGKIFYRRR RRRVKRKSGN IDDFCRRWGG DYRYMVVMDA DSVMSGDCLA
     KLVRLMEANP EAGIIQTAPK ASGMDTLYAR MQQFATRVYG PLFTAGLHFW QLGESHYWGH
     NAIIRMQPFI DHCALAPLPG KGSFAGAILS HDFVEAALMR RAGWGVWIAY DLDGSYEELP
     PNLLDELKRD RRWCHGNLMN FRLFLVKGMH PVHRAVFLTG VMSYLSAPLW FFFLVLSTAL
     LAVHQLMEPQ YFLEPRQLFP IWPQWHPEKA IALFSTTLTL LFLPKLLSVM LIWAKGAKGF
     GGVIRVTLSM LLEMFFSVLL APVRMLFHTR FVLAAFLGWS VQWNSPQRDD DATPWSEAIR
     RHGMQTLLGI AWTLLVAWLN PRFLWWLSPI VGSLILSIPV SVISSRVKLG LRARDEKLFL
     IPEEYDTPRE LRATDEYTYE NRWHALKDGF LKAAVDPLLN ALACAMGTAR HNRAQAIETV
     RGERIGKAID KGPEQLDGAT RLALLSDPVA LSRLHTRVWE EDRDDWLGRW RKAEADDPHA
     ASVPLAQVVP GDAGLLPAAQ S