ID   OPGH_PSEF5              Reviewed;         856 AA.
AC   Q4KJM5;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Glucans biosynthesis glucosyltransferase H {ECO:0000255|HAMAP-Rule:MF_01072};
DE            EC=2.4.1.- {ECO:0000255|HAMAP-Rule:MF_01072};
GN   Name=opgH {ECO:0000255|HAMAP-Rule:MF_01072}; OrderedLocusNames=PFL_0414;
OS   Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=220664;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-477 / NRRL B-23932 / Pf-5;
RX   PubMed=15980861; DOI=10.1038/nbt1110;
RA   Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A.,
RA   Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J.,
RA   Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J.,
RA   Gwinn M.L., Zhou L., Schneider D.J., Cartinhour S.W., Nelson W.C.,
RA   Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III,
RA   Thomashow L.S., Loper J.E.;
RT   "Complete genome sequence of the plant commensal Pseudomonas fluorescens
RT   Pf-5.";
RL   Nat. Biotechnol. 23:873-878(2005).
CC   -!- FUNCTION: Involved in the biosynthesis of osmoregulated periplasmic
CC       glucans (OPGs). {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01072}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01072}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. OpgH
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01072}.
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DR   EMBL; CP000076; AAY95823.1; -; Genomic_DNA.
DR   RefSeq; WP_011058789.1; NC_004129.6.
DR   AlphaFoldDB; Q4KJM5; -.
DR   STRING; 220664.PFL_0414; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   EnsemblBacteria; AAY95823; AAY95823; PFL_0414.
DR   GeneID; 57473403; -.
DR   KEGG; pfl:PFL_0414; -.
DR   PATRIC; fig|220664.5.peg.422; -.
DR   eggNOG; COG2943; Bacteria.
DR   HOGENOM; CLU_015730_1_0_6; -.
DR   OMA; TAGLHYW; -.
DR   OrthoDB; 1049243at2; -.
DR   UniPathway; UPA00637; -.
DR   Proteomes; UP000008540; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009250; P:glucan biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.550.10; -; 1.
DR   HAMAP; MF_01072; MdoH_OpgH; 1.
DR   InterPro; IPR023725; Glucans_biosynth_gluTrFase_H.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Glycosyltransferase; Membrane;
KW   Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..856
FT                   /note="Glucans biosynthesis glucosyltransferase H"
FT                   /id="PRO_1000064610"
FT   TRANSMEM        144..164
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        198..218
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        517..537
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        574..594
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        608..628
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        684..704
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
SQ   SEQUENCE   856 AA;  96394 MW;  97DC0A78A586B773 CRC64;
     MSNSQLQPES LAEYLAHLPM SDQQRAELAG CQSFSELHQR LSSSTFDGPT DAAQASVGQR
     LTLSTAAELE EAEMLAVDAE GRVCLKATPP IRRTKVVPEP WRTNILVRGW RRLTGRTNPP
     APKKDERVLP AARWRTVGSI RRYILLVLML GQTIVAGWYM KGIMPYQGWS FVDLEEVMHQ
     PLMQTAQQVL PYALQTSILI LFGILFCWVS AGFWTALMGF LELLTGHDKY RISGASAGNE
     PIPKDARTAL VMPICNEDVP RVFAGLRATF ESVAATGDLD RFDFFVLSDS NDSDICVAEQ
     QAWLDVCREA KGFGKIFYRR RRRRVKRKSG NLDDFCRRWG GDYKYMVVLD ADSVMSGECL
     TSLVRLMEAT PDAGIIQTAP RASGMDTLYA RMQQFATRVY GPLFTAGLHF WQLGESHYWG
     HNAIIRMQPF IEHCALAPLP GKGAFAGAIL SHDFVEAALM RRAGWGVWIA YDLPGSYEEL
     PPNLLDELKR DRRWCHGNLM NFRLFLVKGM HPVHRAVFLT GVMSYLSAPL WFFFLVLSTA
     LLAVNTLMEP QYFLEPRQLY PLWPQWHPDK AIALFSTTIV LLFLPKLLSI ILIWAKGAKE
     FGGKFKVTLS MLLEMLFSML LAPVRMIFHT RFVLAAFLGW AATWNSPQRD DDSTPWSEAV
     KRHGPQTLLG FCWALLVIWL NPSFLWWLVP IVGSLMLSIP VSVISSRVGL GLKSRDESLF
     LIPEEYAPPQ ALLATDQYTH ENRWHALNDG FVRAVVDPQQ NALACALATS RHTQAEPIEW
     LRQERVRHAL KVGPAALNNS ERLALLSDPV ALGRLHEQVW SEGHSEWLSA WRQSVDADPH
     APLLPLQPLN AAPQPA