ID   OPGH_PSEFS              Reviewed;         856 AA.
AC   C3K816;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Glucans biosynthesis glucosyltransferase H {ECO:0000255|HAMAP-Rule:MF_01072};
DE            EC=2.4.1.- {ECO:0000255|HAMAP-Rule:MF_01072};
GN   Name=opgH {ECO:0000255|HAMAP-Rule:MF_01072}; OrderedLocusNames=PFLU_0374;
OS   Pseudomonas fluorescens (strain SBW25).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=216595;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SBW25;
RX   PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA   Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA   Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M.,
RA   Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA   Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA   Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA   Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA   Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT   "Genomic and genetic analyses of diversity and plant interactions of
RT   Pseudomonas fluorescens.";
RL   Genome Biol. 10:R51.1-R51.16(2009).
CC   -!- FUNCTION: Involved in the biosynthesis of osmoregulated periplasmic
CC       glucans (OPGs). {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01072}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01072}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. OpgH
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AM181176; CAY46651.1; -; Genomic_DNA.
DR   RefSeq; WP_012721785.1; NC_012660.1.
DR   AlphaFoldDB; C3K816; -.
DR   STRING; 294.SRM1_00424; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   EnsemblBacteria; CAY46651; CAY46651; PFLU_0374.
DR   KEGG; pfs:PFLU_0374; -.
DR   PATRIC; fig|216595.4.peg.609; -.
DR   eggNOG; COG2943; Bacteria.
DR   HOGENOM; CLU_015730_1_0_6; -.
DR   OMA; TAGLHYW; -.
DR   OrthoDB; 1049243at2; -.
DR   UniPathway; UPA00637; -.
DR   Proteomes; UP000002332; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009250; P:glucan biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.550.10; -; 1.
DR   HAMAP; MF_01072; MdoH_OpgH; 1.
DR   InterPro; IPR023725; Glucans_biosynth_gluTrFase_H.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Glycosyltransferase; Membrane;
KW   Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..856
FT                   /note="Glucans biosynthesis glucosyltransferase H"
FT                   /id="PRO_1000213477"
FT   TRANSMEM        144..164
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        198..218
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        517..537
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        574..594
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        608..628
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        691..711
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
SQ   SEQUENCE   856 AA;  96298 MW;  32369C6C6AB1DD4F CRC64;
     MSNSQVTPET LSEYLAHLPM TAEQRAELAG CTSFTELHER LSSSTFDAPT DAAQASVGRR
     LTLNTAEELQ DAEMLVLDAS GRVCLKATPP IRRTRVVPEP WRTNILVRGW RRLTGRSNPP
     APPKDERVLP AARWRTVGSI RRYILLVLML GQTIVAGWYM KGIMPYQGWS LVDFDEIRNQ
     TLLQTATQVL PYALQTSILI MFGILFCWVS AGFWTALMGF LELLTGHDKY RISGKSAGDE
     PIPKDARTAL VMPICNEDVP RVFAGLRATF ESVAATGDLD RFDFFVLSDS NDADICIAEQ
     QAWLDVCREA GGFGKIFYRR RRRRVKRKSG NLDDFCRRWG GDYKYMVVLD ADSVMSGECL
     TSLVRLMEAT PDAGIIQTAP RASGMDTLYA RMQQFATRVY GPLFTAGLHF WQLGESHYWG
     HNAIIRMKPF IEHCALAPLP GKGAFAGSIL SHDFVEAALM RRAGWGVWIA YDLPGSYEEL
     PPNLLDELKR DRRWCHGNLM NFRLFLVKGM HPVHRAVFLT GVMSYLSAPL WFLFLVLSTA
     LLAVNTLMEP QYFMAPRQLY PLWPQWHPDK AVALFSTTIV LLFLPKLLSI ILIWAKGAKE
     FGGKFKVTLS MLLEMLFSML LAPVRMIFHT RFVLAAFLGW AATWNSPQRD DDSTPWGEAI
     KRHGTQTLLG FFWALLVVWL NPSFLWWLVP IVGSLMLSIP VSVISSRVGL GLKSRDESLF
     LIPEEYNPPQ ALLSTDKYTH ENRWHALNDG FVRSVVDPQQ NALACALATS RHAQAEPIEY
     LRAERVRHAL KVGPAGLNNG ERLALLSDPV ALARLHEQVW SEGHAEWLAA WRESIKADPH
     APLLPLQPLS SQAQPA