ID   OPGH_PSEP1              Reviewed;         857 AA.
AC   A5WA59;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Glucans biosynthesis glucosyltransferase H {ECO:0000255|HAMAP-Rule:MF_01072};
DE            EC=2.4.1.- {ECO:0000255|HAMAP-Rule:MF_01072};
GN   Name=opgH {ECO:0000255|HAMAP-Rule:MF_01072}; OrderedLocusNames=Pput_4899;
OS   Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=351746;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700007 / DSM 6899 / BCRC 17059 / F1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P.;
RT   "Complete sequence of Pseudomonas putida F1.";
RL   Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the biosynthesis of osmoregulated periplasmic
CC       glucans (OPGs). {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01072}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01072}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. OpgH
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01072}.
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DR   EMBL; CP000712; ABQ81019.1; -; Genomic_DNA.
DR   RefSeq; WP_012053925.1; NC_009512.1.
DR   AlphaFoldDB; A5WA59; -.
DR   STRING; 351746.Pput_4899; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   EnsemblBacteria; ABQ81019; ABQ81019; Pput_4899.
DR   KEGG; ppf:Pput_4899; -.
DR   eggNOG; COG2943; Bacteria.
DR   HOGENOM; CLU_015730_1_0_6; -.
DR   OMA; TAGLHYW; -.
DR   OrthoDB; 1049243at2; -.
DR   UniPathway; UPA00637; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009250; P:glucan biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.550.10; -; 1.
DR   HAMAP; MF_01072; MdoH_OpgH; 1.
DR   InterPro; IPR023725; Glucans_biosynth_gluTrFase_H.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Glycosyltransferase; Membrane;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..857
FT                   /note="Glucans biosynthesis glucosyltransferase H"
FT                   /id="PRO_1000064611"
FT   TRANSMEM        142..162
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        196..216
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        515..535
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        572..592
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        606..626
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        682..702
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
SQ   SEQUENCE   857 AA;  95832 MW;  408907521AB68B9F CRC64;
     MSNSSARPES LGEYLAHLPL SDEQRAELAS CTSFSELHQR LAANPAASSA EAVQASVGPR
     LTVGSAAELE DAEMLGVDGS GRLCLKIAPP IKRTKVVPEP WRTNVLIRMW RRMTGRPNAP
     QPPKRELPPA RWRTVGSIRR YILLALMIGQ TIVAGWYMKG ILPYQGWSFV DFDEIVNQPL
     WDTVVQVWPY ALQTSILILF GILFCWVSAG FWTALMGFLE LLTGRDKYKI SGSSAGNEPI
     APEARTALVM PICNEDVPRV FAGLRATFES VAASGNLDRF DFFVLSDTND TDIAVAEQQA
     WLDVCRETKG FGRIFYRRRR RRVKRKSGNL DDFCRRWGGE YKYMVVLDAD SVMSGECLSS
     LVRLMEANPD AGIIQTGPKA SGMDTLYARM QQFATRVYGP LFTAGLHFWQ LGESHYWGHN
     AIIRMKPFIE HCALAPLPGK GAFAGAILSH DFVEAALMRR AGWGVWIAYD LPGSYEELPP
     NLLDELKRDR RWCHGNLMNF RLFLVKGMHP VHRAVFLTGV MSYLSAPLWF LFLVLSTALL
     ATNTLMEPQY FIEPYQLYPL WPQWHPEKAV ALFSTTIVLL FLPKLLSVIL IWAKGAVEYG
     GRVKVTLSML MEMLFSMLLA PVRMIFHTRF VLAAFLGWAA TWNSPQRDDD STPWGEAVRR
     HGPQTLLGVA WAALVAWLNP SFLWWLAPIV GSLVLSIPVS VISSRTRLGL AAKDEKLFLI
     PEEYATPQEL LATDQYTHEN RWHALHDGFV RAVVDPRQNA LACAMATARH GQAAPIEALR
     AERVAKAIEV GPKGLDLNTR LALLSDPVAL SRLHEQVWAE HNAAWIDVWR ASINNDPHSP
     LLPLHPENAG QPALVGA