ID   OPGH_PSEPF              Reviewed;         856 AA.
AC   Q3KJD8;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Glucans biosynthesis glucosyltransferase H {ECO:0000255|HAMAP-Rule:MF_01072};
DE            EC=2.4.1.- {ECO:0000255|HAMAP-Rule:MF_01072};
GN   Name=opgH {ECO:0000255|HAMAP-Rule:MF_01072}; OrderedLocusNames=Pfl01_0374;
OS   Pseudomonas fluorescens (strain Pf0-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=205922;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pf0-1;
RX   PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA   Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA   Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M.,
RA   Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA   Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA   Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA   Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA   Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT   "Genomic and genetic analyses of diversity and plant interactions of
RT   Pseudomonas fluorescens.";
RL   Genome Biol. 10:R51.1-R51.16(2009).
CC   -!- FUNCTION: Involved in the biosynthesis of osmoregulated periplasmic
CC       glucans (OPGs). {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01072}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01072}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. OpgH
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01072}.
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DR   EMBL; CP000094; ABA72118.1; -; Genomic_DNA.
DR   RefSeq; WP_011332056.1; NC_007492.2.
DR   AlphaFoldDB; Q3KJD8; -.
DR   STRING; 205922.Pfl01_0374; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   EnsemblBacteria; ABA72118; ABA72118; Pfl01_0374.
DR   KEGG; pfo:Pfl01_0374; -.
DR   eggNOG; COG2943; Bacteria.
DR   HOGENOM; CLU_015730_0_0_6; -.
DR   OMA; TAGLHYW; -.
DR   UniPathway; UPA00637; -.
DR   Proteomes; UP000002704; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009250; P:glucan biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.550.10; -; 1.
DR   HAMAP; MF_01072; MdoH_OpgH; 1.
DR   InterPro; IPR023725; Glucans_biosynth_gluTrFase_H.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Glycosyltransferase; Membrane;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..856
FT                   /note="Glucans biosynthesis glucosyltransferase H"
FT                   /id="PRO_1000064612"
FT   TRANSMEM        144..164
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        198..218
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        517..537
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        574..594
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        608..628
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        691..711
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
SQ   SEQUENCE   856 AA;  96642 MW;  B1305529DDE58E5F CRC64;
     MSNSQVQPET LSEYLAHLPM TDEQRAELAG CQSFSELHER LSSKTFDAPT EAAQASVGKR
     LILSTAEELQ DAEMLVLDAS GRVSMKATPP IRRTKVVPEP WRTNILVRGW RRLTGRTNPP
     QPPKDANVLP AARWRTVGSI RRYILLVLML GQTIVAGWYM KGIMPYQGWS FVDLEEVLHQ
     PLLQTATQVL PYALQTSILI MFGILFCWVS AGFWTALMGF LELLTGHDKY RISGKSAGNE
     PIPKDARTAL VMPICNEDVP RVFAGLRATF ESVAATGDLD RFDFFVLSDS NDTDICVAEQ
     QAWLDVCREA KGFGKIFYRR RRRRVKRKSG NLDDFCRRWG GDYKYMVVLD ADSVMSGECL
     TSLVRLMEAT PDAGIIQTAP RASGMDTLYA RMQQFATRVY GPLFTAGLHF WQLGESHYWG
     HNAIIRMKPF IDHCALAPLP GKGAFSGAIL SHDFVEAALM RRAGWGVWIA YDLPGSYEEL
     PPNLLDELKR DRRWCHGNLM NFRLFLVKGM HPVHRAVFLT GVMSYLSAPL WFFFLVLSTA
     LLAVNTLMEP QYFLEPRQLY PLWPQWHPDK AIALFSTTIV LLFLPKLLSI ILIWAKGAKE
     FGGKFKVTLS MLLEMLFSML LAPVRMIFHT RFVLAAFLGW AATWNSPQRD DDSTPWSEAV
     KRHGPQTLLG FFWALLVIWL NPSFLWWLVP IVGSLMLSIP VSVISSRVGL GLKSRDESLF
     LIPEEYNPPQ ALLATDQYTH ENRWHALNDG FVRAVVDPQQ NALACSLATS RHGQAEPIEW
     LRQERVRHAI KVGPAGLNNH DRLQLLSDPV ALARLHEQVW AEGHAEWLDA WRASVKADPH
     APLLPLKPVS LQAQPA