ID   OPGH_PSEPK              Reviewed;         857 AA.
AC   Q88D04;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Glucans biosynthesis glucosyltransferase H {ECO:0000255|HAMAP-Rule:MF_01072};
DE            EC=2.4.1.- {ECO:0000255|HAMAP-Rule:MF_01072};
GN   Name=opgH {ECO:0000255|HAMAP-Rule:MF_01072}; Synonyms=mdoH;
GN   OrderedLocusNames=PP_5025;
OS   Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS   / KT2440).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA   Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA   Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA   Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA   Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA   Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the metabolically
RT   versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
CC   -!- FUNCTION: Involved in the biosynthesis of osmoregulated periplasmic
CC       glucans (OPGs). {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01072}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01072}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. OpgH
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01072}.
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DR   EMBL; AE015451; AAN70590.1; -; Genomic_DNA.
DR   RefSeq; NP_747126.1; NC_002947.4.
DR   RefSeq; WP_010955584.1; NC_002947.4.
DR   AlphaFoldDB; Q88D04; -.
DR   STRING; 160488.PP_5025; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   EnsemblBacteria; AAN70590; AAN70590; PP_5025.
DR   KEGG; ppu:PP_5025; -.
DR   PATRIC; fig|160488.4.peg.5366; -.
DR   eggNOG; COG2943; Bacteria.
DR   HOGENOM; CLU_015730_1_0_6; -.
DR   OMA; TAGLHYW; -.
DR   PhylomeDB; Q88D04; -.
DR   BioCyc; PPUT160488:G1G01-5370-MON; -.
DR   UniPathway; UPA00637; -.
DR   Proteomes; UP000000556; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009250; P:glucan biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.550.10; -; 1.
DR   HAMAP; MF_01072; MdoH_OpgH; 1.
DR   InterPro; IPR023725; Glucans_biosynth_gluTrFase_H.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Glycosyltransferase; Membrane;
KW   Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..857
FT                   /note="Glucans biosynthesis glucosyltransferase H"
FT                   /id="PRO_0000210355"
FT   TRANSMEM        141..158
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        197..219
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        514..536
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        570..592
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        605..627
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        681..703
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
SQ   SEQUENCE   857 AA;  95876 MW;  B6D056E68F85468D CRC64;
     MSNSSARPES LGEYLAHLPL SDEQRAELAS CTSFSELHQR LAANPAASSA EAVQASVGPR
     LTVGSAAELE EAEMLGVDGS GRLCLKIAPP IKRTKVVPEP WRTNVLIRMW RRMTGRPNAP
     QPPKRELPPA RWRTVGSIRR YILLALMIGQ TIVAGWYMKG ILPYQGWSFV DFDEIVNQPL
     WDTVVQVWPY ALQTSILILF GILFCWVSAG FWTALMGFLE LLTGRDKYKI SGSSAGNEPI
     APEARTALVM PICNEDVPRV FAGLRATFES VAASGNLDRF DFFVLSDTND TDIAVAEQQA
     WLDVCRETKG FGRIFYRRRR RRVKRKSGNL DDFCRRWGGE YKYMVVLDAD SVMSGECLSS
     LVRLMEANPD AGIIQTGPKA SGMDTLYARM QQFATRVYGP LFTAGLHFWQ LGESHYWGHN
     AIIRMKPFIE HCALAPLPGK GAFAGAILSH DFVEAALMRR AGWGVWIAYD LPGSYEELPP
     NLLDELKRDR RWCHGNLMNF RLFLVKGMHP VHRAVFLTGV MSYLSAPLWF LFLVLSTALL
     ATNTLMEPQY FIEPYQLYPL WPQWHPEKAV ALFSTTIVLL FLPKLLSVIL IWAKGAVEYG
     GRVKVTLSML MEMLFSMLLA PVRMIFHTRF VLAAFLGWAA TWNSPQRDDD STPWGEAVRR
     HGPQTLLGIA WAALVAWLNP SFLWWLAPIV GSLVLSIPVS VISSRTRLGL AAKDEKLFLI
     PEEYATPQEL LATDQYTHEN RWHALHDGFV RAVVDPRQNA LACAMATARH GQAAPIEALR
     AERVAKAIEV GPKGLDLNTR LALLSDPVAL SRLHEQVWAE HNAAWIDVWR ASINNDPHSP
     LLPLHPENAG QPSLVGA