ID   OPGH_PSESY              Reviewed;         804 AA.
AC   P20401;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 3.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Glucans biosynthesis glucosyltransferase H;
DE            EC=2.4.1.-;
GN   Name=opgH; Synonyms=hrpM;
OS   Pseudomonas syringae pv. syringae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
OX   NCBI_TaxID=321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=R32;
RX   PubMed=3192509; DOI=10.1128/jb.170.12.5479-5488.1988;
RA   Mukhopadhyay P., Williams J., Mills D.;
RT   "Molecular analysis of a pathogenicity locus in Pseudomonas syringae pv.
RT   syringae.";
RL   J. Bacteriol. 170:5479-5488(1988).
CC   -!- FUNCTION: Involved in the biosynthesis of osmoregulated periplasmic
CC       glucans (OPGs). {ECO:0000250}.
CC   -!- FUNCTION: Pathogenicity factor. Required for both the expression of
CC       disease symptoms on host plants and the development of the
CC       hypersensitive reaction on non-host plants.
CC   -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. OpgH
CC       subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=M23555; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
CC       Sequence=M23555; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; M23555; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; B31383; B31383.
DR   AlphaFoldDB; P20401; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   UniPathway; UPA00637; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009250; P:glucan biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.550.10; -; 1.
DR   HAMAP; MF_01072; MdoH_OpgH; 1.
DR   InterPro; IPR023725; Glucans_biosynth_gluTrFase_H.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   2: Evidence at transcript level;
KW   Cell inner membrane; Cell membrane; Glycosyltransferase; Membrane;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..804
FT                   /note="Glucans biosynthesis glucosyltransferase H"
FT                   /id="PRO_0000210357"
FT   TRANSMEM        146..166
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        200..220
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        519..539
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        576..596
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        610..630
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        686..706
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   804 AA;  91130 MW;  208641B684631C4F CRC64;
     MSNSLPVPMS LNEYLAHLPM SDEQRAELAG CTTFAELHER LSAQPVTDPA EAAQASVGRR
     LTLTPRDQLE DAEMLGVDAS GRLCLKATPP IRRTKVVPEP WRTNILVRGW RRLTGKGNPP
     KPEHDDLPRD LPKARWRTVG SIRRYILLIL MLGQTIVAGW YMKGILPYQG WSLVSLDEIT
     RQTFVQTALQ VMPYALQTSI LLLFGILFCW VSAGFWTALM GFLELLTGRD KYRISGASAG
     NEPIEKGART ALVMPICNED VPRVFAGLRA TFESVAATGD LDRFDFFVLS DTNETDIAVA
     EQQAWLDVCR ETKGFGKIFY RRRRRRVKRK SGNLDDFCRR WGGDYRYMVV LDADSVMSGE
     CLTSLVRLME ATPDAGIIQT APRASGMDTL YARMQQFATR VYGPLFTAGL HFWQLGESHY
     WGHNAIIRMK PFIEHCALAP LPGKGAFAGA ILSHDFVEAA LMRRAGWGVW IAYDLPGSYE
     ELPPNLLDEL KRDRRWCHGN LMNFRLFLVK GMHPVHRAVF LTGVMSYLSA PLWFFFLVLS
     TALLAVNTLM EPTYFLEPRQ LYPLWPQWHP EKAVALFSTT IVLLFLPKLL SVILIWAKGA
     KGFGGKFKVT VSMLLEMLFS VLLAPVRMLF HTRFVLAAFL GWAATWNSPQ RDDDSTPWIE
     AVKRHGPQTL LGACWALLVF WLNPSFLWWL APIVVSLMLS IPVSVISSRT NLGVKARDEK
     FFLIPEEFEP PQELISTDRY TYENRWHALK QGFIRAVVDP RQNALACALA TSRHVRLSRL
     KWCVWSVSIR HSRSVRQNSA IRNA