ID   OPGH_VIBC3              Reviewed;         721 AA.
AC   A5F1Q0; C3M048;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Glucans biosynthesis glucosyltransferase H {ECO:0000255|HAMAP-Rule:MF_01072};
DE            EC=2.4.1.- {ECO:0000255|HAMAP-Rule:MF_01072};
GN   Name=opgH {ECO:0000255|HAMAP-Rule:MF_01072}; Synonyms=mdoH;
GN   OrderedLocusNames=VC0395_A0906, VC395_1406;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS   O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA   Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA   Wang W., Wang J., Qian W., Li D., Wang L.;
RT   "A recalibrated molecular clock and independent origins for the cholera
RT   pandemic clones.";
RL   PLoS ONE 3:E4053-E4053(2008).
CC   -!- FUNCTION: Involved in the biosynthesis of osmoregulated periplasmic
CC       glucans (OPGs). {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01072}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01072}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. OpgH
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000627; ABQ21968.1; -; Genomic_DNA.
DR   EMBL; CP001235; ACP09414.1; -; Genomic_DNA.
DR   RefSeq; WP_001881051.1; NZ_JAACZH010000002.1.
DR   AlphaFoldDB; A5F1Q0; -.
DR   STRING; 345073.VC395_1406; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   EnsemblBacteria; ABQ21968; ABQ21968; VC0395_A0906.
DR   GeneID; 57739954; -.
DR   KEGG; vco:VC0395_A0906; -.
DR   KEGG; vcr:VC395_1406; -.
DR   PATRIC; fig|345073.21.peg.1365; -.
DR   eggNOG; COG2943; Bacteria.
DR   HOGENOM; CLU_015730_1_0_6; -.
DR   OMA; TAGLHYW; -.
DR   UniPathway; UPA00637; -.
DR   Proteomes; UP000000249; Chromosome 2.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009250; P:glucan biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.550.10; -; 1.
DR   HAMAP; MF_01072; MdoH_OpgH; 1.
DR   InterPro; IPR025993; Ceramide_glucosylTrfase.
DR   InterPro; IPR023725; Glucans_biosynth_gluTrFase_H.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF13506; Glyco_transf_21; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Glycosyltransferase; Membrane;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..721
FT                   /note="Glucans biosynthesis glucosyltransferase H"
FT                   /id="PRO_1000084505"
FT   TRANSMEM        52..72
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        97..117
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        412..432
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        459..479
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        505..525
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        570..590
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
SQ   SEQUENCE   721 AA;  79880 MW;  F0D3B39AF5EF9F19 CRC64;
     MTNPMVEQGA SQLMGGSAMP PEQHGEMPEQ NLKRLSEGFP RDAIQTGGVK SCSWRRVFVV
     GFALLISAFA IFEMRGVFLV GGLTPIEYAV LVLFAINFCW IALAFSSSIA GFFVLASRKP
     APNTEQPLTT RTAILMPTYN EAPDRVFAAV ETMALALAKT EHGHAFDWFI LSDTTDPEVA
     LSEEQAFWLL RQQTAGKANV YYRRRRKNIA RKAGNIADFC RRWGSGYDHL LVLDADSVMQ
     PSTMISLAQR MQSDPDAGLI QTIPALINGT TLMARVQQFA ARIYGPVVGT GLAWWVQKEG
     NFWGHNAIIR TEAFMSAAGL PHLSGRPPFG GHILSHDFVE AALIRRAGWS VTIAADLSGS
     FEECPPSIID LAVRDRRWCQ GNLQHSRIIG TKGLHWISRL HLTTGIMSYL SSPFWLLLIL
     SGLLLALQAH FIRPEYFTEQ FSLFPTWPVM DSARALQLFY ITMGILFSPK IFGLLLLMFD
     GEMCRTLGGR LRVILSAVTE ILLSALVAPI MMLIHCGAVV SILFGRDSGW APQRRDDGSL
     PIKDLLYRHR WHMTAGVLLG YAAMLDSWTL LAWMSPALIG LWFSVPLSGI TASYTIGAWF
     KQKRILATPE EIETPAIVLA AQARRDEYVV DLQEVWNARM VLADHNLIAL HIAMMDKLPS
     RQPGTAIEPL DAVARIKVQE AESQESLLAL LTKVELSYVL GNPLLIQQVA KLPPSLANQT
     V