ID   OPGH_VIBCH              Reviewed;         721 AA.
AC   Q9KSG9;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Glucans biosynthesis glucosyltransferase H {ECO:0000255|HAMAP-Rule:MF_01072};
DE            EC=2.4.1.- {ECO:0000255|HAMAP-Rule:MF_01072};
GN   Name=opgH {ECO:0000255|HAMAP-Rule:MF_01072}; OrderedLocusNames=VC_1287;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- FUNCTION: Involved in the biosynthesis of osmoregulated periplasmic
CC       glucans (OPGs). {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01072}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01072}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01072}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. OpgH
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01072}.
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DR   EMBL; AE003852; AAF94446.1; -; Genomic_DNA.
DR   PIR; B82218; B82218.
DR   RefSeq; NP_230932.1; NC_002505.1.
DR   RefSeq; WP_001881051.1; NZ_LT906614.1.
DR   AlphaFoldDB; Q9KSG9; -.
DR   STRING; 243277.VC_1287; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   DNASU; 2614741; -.
DR   EnsemblBacteria; AAF94446; AAF94446; VC_1287.
DR   GeneID; 57739954; -.
DR   KEGG; vch:VC_1287; -.
DR   PATRIC; fig|243277.26.peg.1226; -.
DR   eggNOG; COG2943; Bacteria.
DR   HOGENOM; CLU_015730_1_0_6; -.
DR   OMA; TAGLHYW; -.
DR   BioCyc; VCHO:VC1287-MON; -.
DR   UniPathway; UPA00637; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0016758; F:hexosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0009250; P:glucan biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.550.10; -; 1.
DR   HAMAP; MF_01072; MdoH_OpgH; 1.
DR   InterPro; IPR025993; Ceramide_glucosylTrfase.
DR   InterPro; IPR023725; Glucans_biosynth_gluTrFase_H.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF13506; Glyco_transf_21; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Glycosyltransferase; Membrane;
KW   Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..721
FT                   /note="Glucans biosynthesis glucosyltransferase H"
FT                   /id="PRO_0000210365"
FT   TRANSMEM        57..79
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        94..116
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        410..432
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        457..479
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        492..514
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        551..573
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
FT   TRANSMEM        578..600
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01072"
SQ   SEQUENCE   721 AA;  79880 MW;  F0D3B39AF5EF9F19 CRC64;
     MTNPMVEQGA SQLMGGSAMP PEQHGEMPEQ NLKRLSEGFP RDAIQTGGVK SCSWRRVFVV
     GFALLISAFA IFEMRGVFLV GGLTPIEYAV LVLFAINFCW IALAFSSSIA GFFVLASRKP
     APNTEQPLTT RTAILMPTYN EAPDRVFAAV ETMALALAKT EHGHAFDWFI LSDTTDPEVA
     LSEEQAFWLL RQQTAGKANV YYRRRRKNIA RKAGNIADFC RRWGSGYDHL LVLDADSVMQ
     PSTMISLAQR MQSDPDAGLI QTIPALINGT TLMARVQQFA ARIYGPVVGT GLAWWVQKEG
     NFWGHNAIIR TEAFMSAAGL PHLSGRPPFG GHILSHDFVE AALIRRAGWS VTIAADLSGS
     FEECPPSIID LAVRDRRWCQ GNLQHSRIIG TKGLHWISRL HLTTGIMSYL SSPFWLLLIL
     SGLLLALQAH FIRPEYFTEQ FSLFPTWPVM DSARALQLFY ITMGILFSPK IFGLLLLMFD
     GEMCRTLGGR LRVILSAVTE ILLSALVAPI MMLIHCGAVV SILFGRDSGW APQRRDDGSL
     PIKDLLYRHR WHMTAGVLLG YAAMLDSWTL LAWMSPALIG LWFSVPLSGI TASYTIGAWF
     KQKRILATPE EIETPAIVLA AQARRDEYVV DLQEVWNARM VLADHNLIAL HIAMMDKLPS
     RQPGTAIEPL DAVARIKVQE AESQESLLAL LTKVELSYVL GNPLLIQQVA KLPPSLANQT
     V