ID   ARY2_DICDI              Reviewed;         299 AA.
AC   Q54IU8;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Probable arylamine N-acetyltransferase 2;
DE            EC=2.3.1.5;
DE   AltName: Full=Arylamide acetylase 2;
DE   AltName: Full=N-acetyltransferase type 2;
DE            Short=NAT-2;
GN   ORFNames=DDB_G0288507;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + an arylamine = an N-acetylarylamine + CoA;
CC         Xref=Rhea:RHEA:16613, ChEBI:CHEBI:13790, ChEBI:CHEBI:50471,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.5;
CC   -!- SIMILARITY: Belongs to the arylamine N-acetyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000113; EAL63187.1; -; Genomic_DNA.
DR   RefSeq; XP_636690.1; XM_631598.1.
DR   AlphaFoldDB; Q54IU8; -.
DR   SMR; Q54IU8; -.
DR   PaxDb; Q54IU8; -.
DR   EnsemblProtists; EAL63187; EAL63187; DDB_G0288507.
DR   GeneID; 8626662; -.
DR   KEGG; ddi:DDB_G0288507; -.
DR   dictyBase; DDB_G0288507; nat3.
DR   eggNOG; ENOG502RI6E; Eukaryota.
DR   HOGENOM; CLU_049918_4_0_1; -.
DR   InParanoid; Q54IU8; -.
DR   OMA; CVENQET; -.
DR   PhylomeDB; Q54IU8; -.
DR   PRO; PR:Q54IU8; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0004060; F:arylamine N-acetyltransferase activity; IBA:GO_Central.
DR   InterPro; IPR001447; Arylamine_N-AcTrfase.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   PANTHER; PTHR11786; PTHR11786; 1.
DR   Pfam; PF00797; Acetyltransf_2; 1.
DR   PRINTS; PR01543; ANATRNSFRASE.
DR   SUPFAM; SSF54001; SSF54001; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..299
FT                   /note="Probable arylamine N-acetyltransferase 2"
FT                   /id="PRO_0000327945"
FT   ACT_SITE        75
FT                   /note="Acyl-thioester intermediate"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        115
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        130
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   299 AA;  34522 MW;  9852A18C3D1F29EC CRC64;
     MEPNPIFSEF QLEFFKRIKM QPKSIETLND VSEVMSGCAQ IFSFENLDVV ANTTEPLNRE
     VIIQQLVNNK QGGLCYKINS VFYYFIKSFG FNIYQVRCCV ENQETHDCWQ INGAHIINII
     EYENKKYLAD VAFGCNLSYE PIPFSEEIIE SCTGLYRIRK VDNIIRDIKY TYLLEFKKPD
     SFSPESARLW TNGYAFDPLV KAIDIDENDK IDSHATQIQQ LIIDDPTKEF SVKPLATKVV
     NNESIATLTS NSFTLTNCKT GEKTKVTFDI NNEIKQLEQF NQHLISIFNL PPLKFLPKI