OPH_PSESP
ID OPH_PSESP Reviewed; 379 AA.
AC Q9LCQ7;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 49.
DE RecName: Full=Oxidized polyvinyl alcohol hydrolase;
DE Short=OPH;
DE Short=Oxidized PVA hydrolase;
DE EC=3.7.1.7;
DE AltName: Full=Beta-diketone hydrolase;
DE Flags: Precursor;
GN Name=pvaB;
OS Pseudomonas sp.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=306;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC STRAIN=VM15C;
RX PubMed=10746768; DOI=10.1099/00221287-146-3-649;
RA Shimao M., Tamogami T., Kishida S., Harayama S.;
RT "The gene pvaB encodes oxidized polyvinyl alcohol hydrolase of Pseudomonas
RT sp. strain VM15C and forms an operon with the polyvinyl alcohol
RT dehydrogenase gene pvaA.";
RL Microbiology 146:649-657(2000).
CC -!- FUNCTION: Catalyzes the hydrolysis of 4,6-nonanedione, a beta-diketone
CC compound. Also mediates hydrolysis of oxidized polyvinyl alcohol (PVA)
CC in the second step in the degradation of polyvinyl alcohol. Not active
CC toward the monoketone structure. {ECO:0000269|PubMed:10746768}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + nonane-4,6-dione = butanoate + H(+) + pentan-2-one;
CC Xref=Rhea:RHEA:11908, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16111, ChEBI:CHEBI:16472, ChEBI:CHEBI:17968; EC=3.7.1.7;
CC Evidence={ECO:0000269|PubMed:10746768};
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the peptidase S9A family. {ECO:0000305}.
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DR EMBL; AB008494; BAA94192.1; -; Genomic_DNA.
DR PDB; 3WL5; X-ray; 1.60 A; A=30-379.
DR PDB; 3WL6; X-ray; 1.60 A; A/B=30-379.
DR PDB; 3WL7; X-ray; 1.67 A; A=30-379.
DR PDB; 3WL8; X-ray; 1.60 A; A=30-379.
DR PDB; 3WWC; X-ray; 1.49 A; A=30-379.
DR PDB; 3WWD; X-ray; 1.65 A; A=30-379.
DR PDB; 3WWE; X-ray; 2.10 A; A=30-379.
DR PDBsum; 3WL5; -.
DR PDBsum; 3WL6; -.
DR PDBsum; 3WL7; -.
DR PDBsum; 3WL8; -.
DR PDBsum; 3WWC; -.
DR PDBsum; 3WWD; -.
DR PDBsum; 3WWE; -.
DR AlphaFoldDB; Q9LCQ7; -.
DR SMR; Q9LCQ7; -.
DR ESTHER; psesp-OPH; AlphaBeta_hydrolase.
DR BRENDA; 3.7.1.7; 5085.
DR GO; GO:0047699; F:beta-diketone hydrolase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..379
FT /note="Oxidized polyvinyl alcohol hydrolase"
FT /id="PRO_5000049141"
FT ACT_SITE 203
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 309
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT HELIX 54..56
FT /evidence="ECO:0007829|PDB:3WL6"
FT STRAND 61..69
FT /evidence="ECO:0007829|PDB:3WWC"
FT STRAND 72..79
FT /evidence="ECO:0007829|PDB:3WWC"
FT STRAND 86..94
FT /evidence="ECO:0007829|PDB:3WWC"
FT STRAND 97..99
FT /evidence="ECO:0007829|PDB:3WWC"
FT HELIX 101..106
FT /evidence="ECO:0007829|PDB:3WWC"
FT HELIX 108..110
FT /evidence="ECO:0007829|PDB:3WWC"
FT HELIX 113..119
FT /evidence="ECO:0007829|PDB:3WWC"
FT STRAND 122..126
FT /evidence="ECO:0007829|PDB:3WWC"
FT HELIX 129..131
FT /evidence="ECO:0007829|PDB:3WWC"
FT TURN 140..142
FT /evidence="ECO:0007829|PDB:3WWC"
FT STRAND 143..145
FT /evidence="ECO:0007829|PDB:3WWC"
FT STRAND 150..152
FT /evidence="ECO:0007829|PDB:3WWC"
FT TURN 153..156
FT /evidence="ECO:0007829|PDB:3WWE"
FT HELIX 163..169
FT /evidence="ECO:0007829|PDB:3WWC"
FT HELIX 174..186
FT /evidence="ECO:0007829|PDB:3WWC"
FT TURN 187..189
FT /evidence="ECO:0007829|PDB:3WWC"
FT STRAND 192..202
FT /evidence="ECO:0007829|PDB:3WWC"
FT HELIX 204..215
FT /evidence="ECO:0007829|PDB:3WWC"
FT TURN 217..219
FT /evidence="ECO:0007829|PDB:3WWC"
FT STRAND 221..227
FT /evidence="ECO:0007829|PDB:3WWC"
FT HELIX 242..251
FT /evidence="ECO:0007829|PDB:3WWC"
FT STRAND 272..279
FT /evidence="ECO:0007829|PDB:3WWC"
FT STRAND 285..289
FT /evidence="ECO:0007829|PDB:3WWC"
FT STRAND 295..300
FT /evidence="ECO:0007829|PDB:3WWC"
FT HELIX 301..314
FT /evidence="ECO:0007829|PDB:3WWC"
FT STRAND 318..324
FT /evidence="ECO:0007829|PDB:3WWC"
FT STRAND 329..331
FT /evidence="ECO:0007829|PDB:3WWC"
FT HELIX 336..348
FT /evidence="ECO:0007829|PDB:3WWC"
FT HELIX 356..358
FT /evidence="ECO:0007829|PDB:3WWC"
FT STRAND 369..374
FT /evidence="ECO:0007829|PDB:3WWC"
FT STRAND 377..379
FT /evidence="ECO:0007829|PDB:3WWC"
SQ SEQUENCE 379 AA; 40613 MW; 586A83EF32C945A0 CRC64;
MNQSLGVLRL TRGVIALALA SVASGCSSTG ADRTAATPAA ANPAATEPVK WECPAGYEVK
EGLNVDFPHK GMKRAFIVYP AKNVSGPAPV WVPMTGSVES TNDNLTVARS GANSILADHG
YTVIAPVRAC ANQDPNIRGE RCNGPGSNGW NWNPWFEGRA ADPSGEHWKN DEGPDSSFFV
AMVQCVGTKY KLDARRLFLG GISSGGTMTN RALLFRSNFW AGGLPISGEW YVTSDDGTPL
SFDDARAAVA AAPTKIHQGR VGPYPLPAKV GPLIVMTVWG GEKDLWNCTR PDGSRFLCAD
YRPSTQAGSN FFSAQPDVVH VACSSTHGHM WPQLNTQEFN RWALDTLASH PKGSDPRSFK
LTQPPEGYTC HVGPFTGLY
