ID   OPLA_BOVIN              Reviewed;        1288 AA.
AC   Q75WB5;
DT   15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=5-oxoprolinase;
DE            EC=3.5.2.9;
DE   AltName: Full=5-oxo-L-prolinase;
DE            Short=5-OPase;
DE   AltName: Full=Pyroglutamase;
GN   Name=OPLAH;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 3-14, AND TISSUE
RP   SPECIFICITY.
RC   TISSUE=Kidney;
RX   PubMed=14993790; DOI=10.1248/bpb.27.288;
RA   Watanabe T., Abe K., Ishikawa H., Iijima Y.;
RT   "Bovine 5-oxo-L-prolinase: simple assay method, purification, cDNA cloning,
RT   and detection of mRNA in the coronary artery.";
RL   Biol. Pharm. Bull. 27:288-294(2004).
CC   -!- FUNCTION: Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate
CC       coupled to the hydrolysis of ATP to ADP and inorganic phosphate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-oxo-L-proline + ATP + 2 H2O = ADP + H(+) + L-glutamate +
CC         phosphate; Xref=Rhea:RHEA:10348, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58402, ChEBI:CHEBI:456216; EC=3.5.2.9;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in coronary artery and kidney.
CC       {ECO:0000269|PubMed:14993790}.
CC   -!- SIMILARITY: Belongs to the oxoprolinase family. {ECO:0000305}.
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DR   EMBL; AB121737; BAD13433.1; -; mRNA.
DR   RefSeq; NP_001001173.1; NM_001001173.1.
DR   STRING; 9913.ENSBTAP00000022981; -.
DR   PaxDb; Q75WB5; -.
DR   PRIDE; Q75WB5; -.
DR   GeneID; 408006; -.
DR   KEGG; bta:408006; -.
DR   CTD; 26873; -.
DR   eggNOG; KOG1939; Eukaryota.
DR   InParanoid; Q75WB5; -.
DR   OrthoDB; 62698at2759; -.
DR   BRENDA; 3.5.2.9; 908.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0017168; F:5-oxoprolinase (ATP-hydrolyzing) activity; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0006749; P:glutathione metabolic process; IBA:GO_Central.
DR   InterPro; IPR008040; Hydant_A_N.
DR   InterPro; IPR002821; Hydantoinase_A.
DR   InterPro; IPR003692; Hydantoinase_B.
DR   InterPro; IPR045079; Oxoprolinase_fam.
DR   PANTHER; PTHR11365; PTHR11365; 1.
DR   Pfam; PF05378; Hydant_A_N; 1.
DR   Pfam; PF01968; Hydantoinase_A; 1.
DR   Pfam; PF02538; Hydantoinase_B; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Direct protein sequencing; Hydrolase; Nucleotide-binding;
KW   Phosphoprotein; Reference proteome.
FT   CHAIN           1..1288
FT                   /note="5-oxoprolinase"
FT                   /id="PRO_0000208576"
FT   REGION          1249..1268
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         151
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:O14841"
FT   MOD_RES         1265
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P97608"
SQ   SEQUENCE   1288 AA;  137450 MW;  61A97ADC5A1A23B1 CRC64;
     MGGPEGRFHF AIDRGGTFTD VFAQCPGGHV RVLKLLSEDP ANYVDAPTEG IRRILEQEGG
     VLLPRDRPLD TSRIASIRMG TTVATNALLE QQGERVALLV TRGFRDLLHV CTQARAXLFD
     LAVPMPETLY EEVLEVDERV VLYRGXPGAG TPVKGCTGDL LEVQQPVDLG GLRWKLEGLL
     SRGIRSLAVV LMHSYTWAQH EQQVGALARE LGFTHVSLSS EAMPMVRIVP RGHTACADAY
     LTPTIQRYVQ GFRRGFQGQL KDVQVLFMRS DGGLAPMDSF SGSRAVLSGP AGGVVGYSAT
     TYRVEGGQPV IGFDMGGTST DVSRYAGEFE HVFEASTAGV TLQAPQLDIN TVAAGGGSRL
     FFRSGLFVVG PESAGAHPGP ACYRKGGPVT VTDANLVLGR LLPASFPCIF GPGEDQPLSP
     EASRKALEAV ATEVNSFLTN GPCPASPLSL EEVAMGFVRV ANEAMCRPIR ALTQARGHDP
     SAHVLACFGG AGGQHACAIA RALGMDTVHI HRHSGLLSAL GLALADVVHE AQEPCSLPYA
     PETFAQLDQR LGRLEEQCVE ALRAQGFPRS QISTESFLHL RYQGTDCALM VSAHQHPASA
     RSPRAGDFGA AFVERYMREF GFIIPERPVV VDDVRVRGTG SSSLRLEDVP KAHSGPPRVD
     KMTQCYFEGG YQETPVYLLG ELGCGHKLQG PCLIIDSNST ILVEPGCQAE VTETGDIRIS
     VGAETASVVG TQLDPIHLTI FSHRFMSIAE QMGRILQRTA ISTNIKERLD FSCALFGPDG
     GLVSNVPHIP VHLGAMQETV QFQIQQLGAD LHPGDVLLSN HPSAGGSHLP DLTVITPVFW
     PGQTRPVFYV ASRGHHADIG GITPGSMPPH STSLQQEGAV FLSFKLVHGG VFQEEAVTEA
     LRAPGKIPGC SGTRNLHDNL SDLRAQVAAN QKGIQLVGEL IGQYGLDVVQ AYMGHIQANA
     ELAVRDMLRA FGTARQARGL PLEVSAEDHM DDGSPIRLRV QINMSQGSAV FDFSGSGPEV
     FGNLNAPRAI TLSALIYCLR CLVGRDIPLN QGCLAPVRVV IPKGSILDPS PDAAVVGGNV
     LTSQRVVDVI LGAFGACAAS QGCMNNVTLG NAHMGYYETV AGGAGAGPGW HGRSGVHSHM
     TNTRITDPEI LESRYPVILR RFELRLGSGG RGRFRGGDGI IRELLFREEA LLSVLTERRA
     FQPYGLMGGE PGARGLNLLI RKDGRTVNLG GKTSVPVYPG DVFCLHTPGG GGYGDPGDPA
     PLPGSPLQPL AFPERGSVYE YRRAQEAV