OPN4A_DANRE
ID OPN4A_DANRE Reviewed; 593 AA.
AC Q2KNE5; B3DH91;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 3.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Melanopsin-A;
DE AltName: Full=Mammalian-like melanopsin;
DE AltName: Full=Melanopsin-M;
DE AltName: Full=Opsin-4-A;
DE AltName: Full=Opsin-4M;
GN Name=opn4a; Synonyms=opn4, opn4d {ECO:0000312|EMBL:AAX73256.1}, opn4m1;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAX73256.1}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 74-430.
RC TISSUE=Retina {ECO:0000312|EMBL:AAX73256.1};
RX PubMed=16856781; DOI=10.1371/journal.pbio.0040254;
RA Bellingham J., Chaurasia S.S., Melyan Z., Liu C., Cameron M.A.,
RA Tarttelin E.E., Iuvone P.M., Hankins M.W., Tosini G., Lucas R.J.;
RT "Evolution of melanopsin photoreceptors: discovery and characterization of
RT a new melanopsin in nonmammalian vertebrates.";
RL PLoS Biol. 4:1334-1343(2006).
CC -!- FUNCTION: Photoreceptor implicated in non-image-forming responses to
CC light. May be able to isomerize covalently bound all-trans retinal back
CC to 11-cis retinal (By similarity). {ECO:0000250|UniProtKB:Q9QXZ9}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9QXZ9};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Opsin
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; BX897719; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC162681; AAI62681.1; -; mRNA.
DR EMBL; AY882945; AAX73256.1; -; mRNA.
DR RefSeq; NP_001122233.1; NM_001128761.1.
DR AlphaFoldDB; Q2KNE5; -.
DR SMR; Q2KNE5; -.
DR STRING; 7955.ENSDARP00000109133; -.
DR PaxDb; Q2KNE5; -.
DR Ensembl; ENSDART00000185271; ENSDARP00000148289; ENSDARG00000110010.
DR GeneID; 571624; -.
DR KEGG; dre:571624; -.
DR CTD; 571624; -.
DR ZFIN; ZDB-GENE-070111-2; opn4a.
DR eggNOG; KOG3656; Eukaryota.
DR InParanoid; Q2KNE5; -.
DR OMA; PNCTRIV; -.
DR OrthoDB; 911005at2759; -.
DR PhylomeDB; Q2KNE5; -.
DR Reactome; R-DRE-416476; G alpha (q) signalling events.
DR Reactome; R-DRE-419771; Opsins.
DR PRO; PR:Q2KNE5; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR Bgee; ENSDARG00000110010; Expressed in inner nuclear layer of retina and 6 other tissues.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0008020; F:G protein-coupled photoreceptor activity; ISS:ZFIN.
DR GO; GO:0009881; F:photoreceptor activity; IDA:ZFIN.
DR GO; GO:0071482; P:cellular response to light stimulus; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:ZFIN.
DR GO; GO:0007602; P:phototransduction; IBA:GO_Central.
DR GO; GO:0007601; P:visual perception; IEA:InterPro.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR001760; Opsin.
DR InterPro; IPR027430; Retinal_BS.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00238; OPSIN.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR PROSITE; PS00238; OPSIN; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Chromophore; Disulfide bond; G-protein coupled receptor;
KW Membrane; Photoreceptor protein; Receptor; Reference proteome;
KW Retinal protein; Sensory transduction; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..593
FT /note="Melanopsin-A"
FT /id="PRO_0000271894"
FT TOPO_DOM 1..77
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 99..112
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 134..150
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 172..191
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 192..212
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 213..243
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 265..300
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 301..321
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 322..336
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 337..357
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 358..593
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 397..449
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 547..593
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 547..575
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 344
FT /note="N6-(retinylidene)lysine"
FT /evidence="ECO:0000250"
FT DISULFID 148..226
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 378
FT /note="V -> I (in Ref. 2; AAI62681)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 593 AA; 65529 MW; D4434772E5301B17 CRC64;
MMSGAAHSVR KGISCPTQDP NCTRIVESLS AWNDSVMSAY RLVDLPPTTT TTTSVAMVEE
SVYPFPTVDV PDHAHYTIGA VILTVGITGM LGNFLVIYAF SRSRTLRTPA NLFIINLAIT
DFLMCATQAP IFFTTSMHKR WIFGEKGCEL YAFCGALFGI CSMITLMVIA VDRYFVITRP
LASIGVLSQK RALLILLVAW VYSLGWSLPP FFGWSAYVPE GLLTSCTWDY MTFTPSVRAY
TMLLFIFVFF IPLIVIIYCY FFIFRSIRTT NEAVGKINGD NKRDSMKRFQ RLKNEWKMAK
IALIVILMYV ISWSPYSTVA LTAFAGYSDF LTPYMNSVPA VIAKASAIHN PIIYAITHPK
YRLAIAKYIP CLRLLLCVPK RDLHSFHSSL MSTRRSTVTS QSSDMSGRFR RTSTGKSRLS
SASDSESGWT DTEADLSSMS SRPASRQVSC DISKDTAEMP DFKPCNSSSF KSKLKSHDSG
IFEKSSSDVD DVSVAGIIQP DRTLTNAGDI TDVPISRGAI GRIPSIVITS ESSSLLPSVR
PTYRISRSNV STVGTNPARR DSRGGVQQGA AHLSNAAETP ESGHIDNHRP QYL
