ARY2_MOUSE
ID ARY2_MOUSE Reviewed; 290 AA.
AC P50295;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Arylamine N-acetyltransferase 2;
DE EC=2.3.1.5 {ECO:0000269|PubMed:7545952};
DE AltName: Full=Arylamide acetylase 2;
DE AltName: Full=N-acetyltransferase type 2;
DE Short=NAT-2;
GN Name=Nat2; Synonyms=Aac2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE, AND VARIANT ILE-99.
RC STRAIN=A/J, and C57BL/6J;
RX PubMed=1875909;
RA Martell K.J., Vatsis K.P., Weber W.W.;
RT "Molecular genetic basis of rapid and slow acetylation in mice.";
RL Mol. Pharmacol. 40:218-227(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C3H/HeJ; TISSUE=Heart;
RA Hein D.W., Doll M.A.;
RL Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE, AND CATALYTIC ACTIVITY.
RC STRAIN=BALB/cJ; TISSUE=Liver;
RX PubMed=7545952; DOI=10.1042/bj3020347;
RA Kelly S.L., Sim E.;
RT "Arylamine N-acetyltransferase in Balb/c mice: identification of a novel
RT mouse isoenzyme by cloning and expression in vitro.";
RL Biochem. J. 302:347-353(1994).
RN [4]
RP CHARACTERIZATION.
RX PubMed=1513324;
RA Martell K.J., Levy G.N., Weber W.W.;
RT "Cloned mouse N-acetyltransferases: enzymatic properties of expressed Nat-1
RT and Nat-2 gene products.";
RL Mol. Pharmacol. 42:265-272(1992).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Kidney, Liver, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Participates in the detoxification of a plethora of hydrazine
CC and arylamine drugs. 2-aminofluorene and p-aminobenzoic acid (PABA) are
CC preferred substrates for NAT-2. Less activity with anisidine and barely
CC detectable with SMZ.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + an arylamine = an N-acetylarylamine + CoA;
CC Xref=Rhea:RHEA:16613, ChEBI:CHEBI:13790, ChEBI:CHEBI:50471,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.5;
CC Evidence={ECO:0000269|PubMed:7545952};
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- POLYMORPHISM: There are two forms of NAT2: a rapid/stable isoform (Asn-
CC 99) and a slow/unstable isoform (Ile-99). {ECO:0000269|PubMed:1875909}.
CC -!- SIMILARITY: Belongs to the arylamine N-acetyltransferase family.
CC {ECO:0000305}.
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DR EMBL; U35886; AAA78943.1; -; mRNA.
DR EMBL; U35887; AAA78944.1; -; mRNA.
DR EMBL; U37249; AAA80353.1; -; Genomic_DNA.
DR EMBL; U37250; AAA80354.1; -; Genomic_DNA.
DR CCDS; CCDS22338.1; -.
DR PIR; B61267; B61267.
DR RefSeq; NP_001162049.1; NM_001168577.1.
DR RefSeq; NP_035004.1; NM_010874.3.
DR AlphaFoldDB; P50295; -.
DR SMR; P50295; -.
DR STRING; 10090.ENSMUSP00000130065; -.
DR BindingDB; P50295; -.
DR ChEMBL; CHEMBL5724; -.
DR iPTMnet; P50295; -.
DR PhosphoSitePlus; P50295; -.
DR SwissPalm; P50295; -.
DR EPD; P50295; -.
DR jPOST; P50295; -.
DR PaxDb; P50295; -.
DR PeptideAtlas; P50295; -.
DR PRIDE; P50295; -.
DR ProteomicsDB; 281807; -.
DR DNASU; 17961; -.
DR Ensembl; ENSMUST00000093470; ENSMUSP00000091181; ENSMUSG00000051147.
DR Ensembl; ENSMUST00000163856; ENSMUSP00000130065; ENSMUSG00000051147.
DR GeneID; 17961; -.
DR KEGG; mmu:17961; -.
DR UCSC; uc009lvw.2; mouse.
DR CTD; 10; -.
DR MGI; MGI:109201; Nat2.
DR VEuPathDB; HostDB:ENSMUSG00000051147; -.
DR eggNOG; ENOG502RD0D; Eukaryota.
DR GeneTree; ENSGT00390000012054; -.
DR HOGENOM; CLU_049918_3_0_1; -.
DR InParanoid; P50295; -.
DR OMA; CYEHNTL; -.
DR OrthoDB; 1545569at2759; -.
DR PhylomeDB; P50295; -.
DR TreeFam; TF106311; -.
DR BRENDA; 2.3.1.5; 3474.
DR Reactome; R-MMU-156582; Acetylation.
DR Reactome; R-MMU-9753281; Paracetamol ADME.
DR BioGRID-ORCS; 17961; 1 hit in 71 CRISPR screens.
DR PRO; PR:P50295; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; P50295; protein.
DR Bgee; ENSMUSG00000051147; Expressed in small intestine Peyer's patch and 193 other tissues.
DR ExpressionAtlas; P50295; baseline and differential.
DR Genevisible; P50295; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0004060; F:arylamine N-acetyltransferase activity; IDA:MGI.
DR GO; GO:0008080; F:N-acetyltransferase activity; ISO:MGI.
DR InterPro; IPR001447; Arylamine_N-AcTrfase.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR PANTHER; PTHR11786; PTHR11786; 1.
DR Pfam; PF00797; Acetyltransf_2; 1.
DR PRINTS; PR01543; ANATRNSFRASE.
DR SUPFAM; SSF54001; SSF54001; 1.
PE 1: Evidence at protein level;
KW Acyltransferase; Cytoplasm; Reference proteome; Transferase.
FT CHAIN 1..290
FT /note="Arylamine N-acetyltransferase 2"
FT /id="PRO_0000107909"
FT ACT_SITE 68
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000250"
FT ACT_SITE 107
FT /evidence="ECO:0000250"
FT ACT_SITE 122
FT /evidence="ECO:0000250"
FT BINDING 103
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
FT BINDING 104
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
FT BINDING 106..107
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 208
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
FT VARIANT 99
FT /note="N -> I (in allele NAT2*9; slow/unstable isoform)"
SQ SEQUENCE 290 AA; 33701 MW; 704E000DE48CE557 CRC64;
MDIEAYFERI GYQSTRSKLD LKTLTEILQH QIRAIPFENL NIHCGESMEL SLEAIFDQIV
RKKRGGWCLQ VNHLLYWALT KLGFETTMLG GYVFNTPANK YSSGMIHLLV QVTISGKDYI
VDAGFGRSYQ MWEPLELTSG KDQPQVPAIF RLTEENGTWY LDQIRREQYV PNQEFINSDL
LEKNKYRKIY SFTLEPRTIE DFESMNTYLQ TSPASVFTSK SFCSLQTPEG VHCLVGSTLT
YRRFSYKDNV DLVEFKSLTE EEIEDVLRTI FGVSLERKLV PKHGDRFFTI
