OPN5_HUMAN
ID OPN5_HUMAN Reviewed; 354 AA.
AC Q6U736; A0AV33; Q5T5B9; Q5T886; Q7Z603; Q86SL5;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 3.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Opsin-5;
DE AltName: Full=G-protein coupled receptor 136;
DE AltName: Full=G-protein coupled receptor PGR12;
DE AltName: Full=Neuropsin;
DE AltName: Full=Transmembrane protein 13;
GN Name=OPN5; Synonyms=GPR136, PGR12, TMEM13;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=14623103; DOI=10.1016/s0014-5793(03)01212-2;
RA Tarttelin E.E., Bellingham J., Hankins M.W., Foster R.G., Lucas R.J.;
RT "Neuropsin (Opn5): a novel opsin identified in mammalian neural tissue.";
RL FEBS Lett. 554:410-416(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=14623098; DOI=10.1016/s0014-5793(03)01196-7;
RA Fredriksson R., Hoeglund P.J., Gloriam D.E.I., Lagerstroem M.C.,
RA Schioeth H.B.;
RT "Seven evolutionarily conserved human rhodopsin G protein-coupled receptors
RT lacking close relatives.";
RL FEBS Lett. 554:381-388(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 119-231.
RX PubMed=12679517; DOI=10.1073/pnas.0230374100;
RA Vassilatis D.K., Hohmann J.G., Zeng H., Li F., Ranchalis J.E.,
RA Mortrud M.T., Brown A., Rodriguez S.S., Weller J.R., Wright A.C.,
RA Bergmann J.E., Gaitanaris G.A.;
RT "The G protein-coupled receptor repertoires of human and mouse.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:4903-4908(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP FUNCTION.
RX PubMed=22043319; DOI=10.1371/journal.pone.0026388;
RA Kojima D., Mori S., Torii M., Wada A., Morishita R., Fukada Y.;
RT "UV-sensitive photoreceptor protein OPN5 in humans and mice.";
RL PLoS ONE 6:e26388-e26388(2011).
CC -!- FUNCTION: G-protein coupled receptor which selectively activates G(i)
CC type G proteins via ultraviolet A (UVA) light-mediated activation in
CC the retina (By similarity). Preferentially binds the chromophore 11-cis
CC retinal and is a bistable protein that displays emission peaks at 380
CC nm (UVA light) and 470 nm (blue light) (PubMed:22043319). Required for
CC the light-response in the inner plexiform layer, and contributes to the
CC regulation of the light-response in the nerve fiber layer, via
CC phosphorylated DAT/SLC6A3 dopamine uptake (By similarity). Involved in
CC local corneal and retinal circadian rhythm photoentrainment via
CC modulation of the UVA light-induced phase-shift of the retina clock (By
CC similarity). Acts as a circadian photoreceptor in the outer ear, via
CC modulation of circadian clock-gene expression in response to violet
CC light during the light-to-dark transition phase and night phase of the
CC circadian cycle (By similarity). Required in the retina to negatively
CC regulate hyaloid vessel regression during postnatal development via
CC light-dependent OPN5-SLC32A1-DRD2-VEGFR2 signaling (By similarity).
CC Involved in the light-dependent regulation of retina and vitreous
CC compartment dopamine levels (By similarity).
CC {ECO:0000250|UniProtKB:Q6VZZ7, ECO:0000269|PubMed:22043319}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Detected in brain and retina and cell lines derived
CC from neural retina. {ECO:0000269|PubMed:14623103}.
CC -!- PTM: It is uncertain whether Cys-315 or Cys-316 is palmitoylated.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Opsin
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAI20454.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY377391; AAR21109.1; -; mRNA.
DR EMBL; AY288419; AAP72128.1; -; mRNA.
DR EMBL; AY255615; AAO85127.1; -; mRNA.
DR EMBL; AL356421; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL161622; CAI20454.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BC126194; AAI26195.1; -; mRNA.
DR EMBL; BC126198; AAI26199.1; -; mRNA.
DR CCDS; CCDS4923.1; -.
DR RefSeq; NP_859528.1; NM_181744.3.
DR AlphaFoldDB; Q6U736; -.
DR SMR; Q6U736; -.
DR BioGRID; 128717; 3.
DR STRING; 9606.ENSP00000360255; -.
DR ChEMBL; CHEMBL4523878; -.
DR GlyGen; Q6U736; 1 site.
DR iPTMnet; Q6U736; -.
DR PhosphoSitePlus; Q6U736; -.
DR BioMuta; OPN5; -.
DR DMDM; 116242690; -.
DR PaxDb; Q6U736; -.
DR PRIDE; Q6U736; -.
DR Antibodypedia; 16965; 234 antibodies from 30 providers.
DR DNASU; 221391; -.
DR Ensembl; ENST00000371211.6; ENSP00000360255.2; ENSG00000124818.15.
DR Ensembl; ENST00000638973.1; ENSP00000491960.1; ENSG00000124818.15.
DR GeneID; 221391; -.
DR KEGG; hsa:221391; -.
DR MANE-Select; ENST00000371211.7; ENSP00000360255.2; NM_181744.4; NP_859528.1.
DR UCSC; uc003ozc.4; human.
DR CTD; 221391; -.
DR DisGeNET; 221391; -.
DR GeneCards; OPN5; -.
DR HGNC; HGNC:19992; OPN5.
DR HPA; ENSG00000124818; Tissue enriched (testis).
DR MIM; 609042; gene.
DR neXtProt; NX_Q6U736; -.
DR OpenTargets; ENSG00000124818; -.
DR PharmGKB; PA134942887; -.
DR VEuPathDB; HostDB:ENSG00000124818; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01020000230391; -.
DR InParanoid; Q6U736; -.
DR OrthoDB; 704940at2759; -.
DR PhylomeDB; Q6U736; -.
DR TreeFam; TF324998; -.
DR PathwayCommons; Q6U736; -.
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR Reactome; R-HSA-419771; Opsins.
DR BioGRID-ORCS; 221391; 14 hits in 1063 CRISPR screens.
DR ChiTaRS; OPN5; human.
DR GeneWiki; OPN5; -.
DR GenomeRNAi; 221391; -.
DR Pharos; Q6U736; Tbio.
DR PRO; PR:Q6U736; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q6U736; protein.
DR Bgee; ENSG00000124818; Expressed in left testis and 40 other tissues.
DR ExpressionAtlas; Q6U736; baseline and differential.
DR Genevisible; Q6U736; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0005502; F:11-cis retinal binding; IMP:UniProtKB.
DR GO; GO:0008020; F:G protein-coupled photoreceptor activity; ISS:UniProtKB.
DR GO; GO:0071482; P:cellular response to light stimulus; ISS:UniProtKB.
DR GO; GO:0071492; P:cellular response to UV-A; IDA:UniProtKB.
DR GO; GO:0043153; P:entrainment of circadian clock by photoperiod; ISS:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:1990384; P:hyaloid vascular plexus regression; ISS:UniProtKB.
DR GO; GO:0007602; P:phototransduction; ISS:UniProtKB.
DR GO; GO:0007604; P:phototransduction, UV; IMP:UniProtKB.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR002962; Peropsin.
DR InterPro; IPR027430; Retinal_BS.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01244; PEROPSIN.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR PROSITE; PS00238; OPSIN; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Chromophore; Disulfide bond; G-protein coupled receptor;
KW Glycoprotein; Lipoprotein; Membrane; Palmitate; Photoreceptor protein;
KW Receptor; Reference proteome; Retinal protein; Sensory transduction;
KW Transducer; Transmembrane; Transmembrane helix; Vision.
FT CHAIN 1..354
FT /note="Opsin-5"
FT /id="PRO_0000197817"
FT TOPO_DOM 1..33
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 55..74
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..95
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 96..108
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 130..150
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 172..197
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 198..218
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 219..252
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..273
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 274..288
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..309
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 310..353
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 296
FT /note="N6-(retinylidene)lysine"
FT LIPID 315
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT LIPID 316
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT CARBOHYD 4
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 106..183
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 84..86
FT /note="Missing (in Ref. 2; AAP72128)"
FT /evidence="ECO:0000305"
FT CONFLICT 133..145
FT /note="Missing (in Ref. 3; AAO85127)"
FT /evidence="ECO:0000305"
FT CONFLICT 353..354
FT /note="WE -> V (in Ref. 2; AAP72128 and 4; CAI20454)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 354 AA; 39727 MW; BBF03FA2C0741005 CRC64;
MALNHTALPQ DERLPHYLRD GDPFASKLSW EADLVAGFYL TIIGILSTFG NGYVLYMSSR
RKKKLRPAEI MTINLAVCDL GISVVGKPFT IISCFCHRWV FGWIGCRWYG WAGFFFGCGS
LITMTAVSLD RYLKICYLSY GVWLKRKHAY ICLAAIWAYA SFWTTMPLVG LGDYVPEPFG
TSCTLDWWLA QASVGGQVFI LNILFFCLLL PTAVIVFSYV KIIAKVKSSS KEVAHFDSRI
HSSHVLEMKL TKVAMLICAG FLIAWIPYAV VSVWSAFGRP DSIPIQLSVV PTLLAKSAAM
YNPIIYQVID YKFACCQTGG LKATKKKSLE GFRLHTVTTV RKSSAVLEIH EEWE
