ID   OPR2_ORYSJ              Reviewed;         376 AA.
AC   Q69TH4; A0A0P0WUE5;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Putative 12-oxophytodienoate reductase 2;
DE            EC=1.3.1.-;
DE   AltName: Full=OPDA-reductase 2;
DE            Short=OsOPR2;
GN   Name=OPR2; OrderedLocusNames=Os06g0216200, LOC_Os06g11280;
GN   ORFNames=OsJ_20594, OSJNBb0024N18.12, P0537F07.34;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- FUNCTION: Putative oxophytodienoate reductase that may be involved in
CC       the biosynthesis or metabolism of oxylipin signaling molecules.
CC       {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC   -!- SIMILARITY: Belongs to the NADH:flavin oxidoreductase/NADH oxidase
CC       family. {ECO:0000305}.
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DR   EMBL; AP003525; BAD35325.1; -; Genomic_DNA.
DR   EMBL; AP004741; BAD35833.1; -; Genomic_DNA.
DR   EMBL; AP008212; BAF19058.1; -; Genomic_DNA.
DR   EMBL; AP014962; BAS96787.1; -; Genomic_DNA.
DR   EMBL; CM000143; EEE65331.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q69TH4; -.
DR   SMR; Q69TH4; -.
DR   STRING; 4530.OS06T0216200-00; -.
DR   PRIDE; Q69TH4; -.
DR   EnsemblPlants; Os06t0216200-00; Os06t0216200-00; Os06g0216200.
DR   Gramene; Os06t0216200-00; Os06t0216200-00; Os06g0216200.
DR   eggNOG; KOG0134; Eukaryota.
DR   HOGENOM; CLU_012153_0_0_1; -.
DR   InParanoid; Q69TH4; -.
DR   OMA; VWGADRI; -.
DR   Proteomes; UP000000763; Chromosome 6.
DR   Proteomes; UP000007752; Chromosome 6.
DR   Proteomes; UP000059680; Chromosome 6.
DR   Genevisible; Q69TH4; OS.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0009695; P:jasmonic acid biosynthetic process; IBA:GO_Central.
DR   GO; GO:0031408; P:oxylipin biosynthetic process; IBA:GO_Central.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR001155; OxRdtase_FMN_N.
DR   InterPro; IPR045247; Oye-like.
DR   PANTHER; PTHR22893; PTHR22893; 1.
DR   Pfam; PF00724; Oxidored_FMN; 1.
PE   3: Inferred from homology;
KW   Fatty acid biosynthesis; Fatty acid metabolism; Flavoprotein; FMN;
KW   Lipid biosynthesis; Lipid metabolism; NADP; Oxidoreductase;
KW   Oxylipin biosynthesis; Reference proteome.
FT   CHAIN           1..376
FT                   /note="Putative 12-oxophytodienoate reductase 2"
FT                   /id="PRO_0000410708"
FT   ACT_SITE        183
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         31..33
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         64
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         106
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         178..181
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         230
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         301
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         322..323
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   376 AA;  42068 MW;  5E47F7BBBBE108D8 CRC64;
     MVHAPAKEAI PLLTPYKMGQ LELSHRVVLA PLTRCRSYGH VPQPHAAVYY SQRATNGGLL
     IAEATVISPT AQGYPDTPGI YTQQQIEAWK PIVDAVHRKG ALFFLQIWHV GRVSTTDFQP
     NGQAPISSTD KQITPDDSGM VYSKPRRLRT DEIPQIVDDF RRAARNAIES GFDGVEIHGA
     HGYLLDQFMK DSANDRTDEY GGNLENRCRF AVEVIDAVVA EVGAHRVGIR LSPFDNYMDC
     FDSNPVALGS YMVQQLNKHP GFLYCHMVEP GMAIVEGRRK ITHGLLPFRK QFNGTFIAAG
     GYDREEGNKV VADGYADLVA YGRLFLANPD LPRRFELDAP LNRYDRSTFY TQDPVVGYTD
     YPFLEEIDEE STTTYA