ID   OPR4_ORYSJ              Reviewed;         380 AA.
AC   Q69TH8; A0A0P0WUK4; Q0DDL8;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Putative 12-oxophytodienoate reductase 4;
DE            EC=1.3.1.-;
DE   AltName: Full=OPDA-reductase 4;
DE            Short=OsOPR4;
GN   Name=OPR4; Synonyms=OPR10; OrderedLocusNames=Os06g0215900, LOC_Os06g11240;
GN   ORFNames=OsJ_20592, OSJNBb0024N18.8, P0537F07.30;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Putative oxophytodienoate reductase that may be involved in
CC       the biosynthesis or metabolism of oxylipin signaling molecules.
CC       {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC   -!- SIMILARITY: Belongs to the NADH:flavin oxidoreductase/NADH oxidase
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF19055.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AP003525; BAD35321.1; -; Genomic_DNA.
DR   EMBL; AP004741; BAD35829.1; -; Genomic_DNA.
DR   EMBL; AP008212; BAF19055.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014962; BAS96783.1; -; Genomic_DNA.
DR   EMBL; CM000143; EAZ36271.1; -; Genomic_DNA.
DR   EMBL; AK105590; BAG97304.1; -; mRNA.
DR   RefSeq; XP_015640930.1; XM_015785444.1.
DR   AlphaFoldDB; Q69TH8; -.
DR   SMR; Q69TH8; -.
DR   STRING; 4530.OS06T0215900-02; -.
DR   PaxDb; Q69TH8; -.
DR   PRIDE; Q69TH8; -.
DR   EnsemblPlants; Os06t0215900-02; Os06t0215900-02; Os06g0215900.
DR   GeneID; 4340486; -.
DR   Gramene; Os06t0215900-02; Os06t0215900-02; Os06g0215900.
DR   KEGG; osa:4340486; -.
DR   eggNOG; KOG0134; Eukaryota.
DR   InParanoid; Q69TH8; -.
DR   OMA; RWFIANP; -.
DR   OrthoDB; 978998at2759; -.
DR   Proteomes; UP000000763; Chromosome 6.
DR   Proteomes; UP000007752; Chromosome 6.
DR   Proteomes; UP000059680; Chromosome 6.
DR   ExpressionAtlas; Q69TH8; baseline and differential.
DR   Genevisible; Q69TH8; OS.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0009695; P:jasmonic acid biosynthetic process; IBA:GO_Central.
DR   GO; GO:0031408; P:oxylipin biosynthetic process; IBA:GO_Central.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR001155; OxRdtase_FMN_N.
DR   InterPro; IPR045247; Oye-like.
DR   PANTHER; PTHR22893; PTHR22893; 1.
DR   Pfam; PF00724; Oxidored_FMN; 1.
PE   2: Evidence at transcript level;
KW   Fatty acid biosynthesis; Fatty acid metabolism; Flavoprotein; FMN;
KW   Lipid biosynthesis; Lipid metabolism; NADP; Oxidoreductase;
KW   Oxylipin biosynthesis; Reference proteome.
FT   CHAIN           1..380
FT                   /note="Putative 12-oxophytodienoate reductase 4"
FT                   /id="PRO_0000410710"
FT   ACT_SITE        188
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         36..38
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         69
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         111
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         183..186
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         235
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         276
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         306
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
FT   BINDING         327..328
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   380 AA;  42432 MW;  8A6E1C007CC6B5A4 CRC64;
     MAREAEKDAA AAAEIPLLTP YKMGRFELSH RVVLAPLTRN RSYGNVPRPH AVLYYTQRAT
     SGGLLVTEAT GVSDTAQGYP DTPGIWTQQQ VEAWKPIVDA VHRKGALFIC QLWHVGRVST
     NEYQPDGQAP ISSTDRQITP DDSGIVYSKP RRLRTEEIPQ IIDDFRRAAR NAIEAGFDGV
     EIHGAHGYLL EQFMKDSAND RSDEYGGSLE NRCRFVVEVI DAIVAEVGAH RVGIRLSPFI
     DYMDCVDSDP VALGSYMVQQ LNKHPGFLYC HMVEPRMAIV EGRRKITHGL LPFRKLFNGT
     FIAAGGYDRE EGNKVIADGY ADLVAYGRHF LANPDLPKRF AINAPLNKYN RSTFYIQDPV
     VGYTDYPFLD EKDEGAATYA