OPRJ_PSEAE
ID OPRJ_PSEAE Reviewed; 479 AA.
AC Q51397;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Outer membrane protein OprJ;
DE Flags: Precursor;
GN Name=oprJ; OrderedLocusNames=PA4597;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PAO;
RX PubMed=8878035; DOI=10.1046/j.1365-2958.1996.281397.x;
RA Poole K., Gotoh N., Tsujimoto H., Zhao Q., Wada A., Yamasaki T., Neshat S.,
RA Yamagishi J., Li X.Z., Nishino T.;
RT "Overexpression of the mexC-mexD-oprJ efflux operon in nfxB-type multidrug-
RT resistant strains of Pseudomonas aeruginosa.";
RL Mol. Microbiol. 21:713-724(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Channel-forming component of a multidrug resistance efflux
CC pump.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the outer membrane factor (OMF) (TC 1.B.17)
CC family. {ECO:0000305}.
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DR EMBL; U57969; AAB41958.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG07985.1; -; Genomic_DNA.
DR PIR; G83071; G83071.
DR RefSeq; NP_253287.1; NC_002516.2.
DR RefSeq; WP_003112802.1; NZ_QZGE01000004.1.
DR PDB; 5AZS; X-ray; 3.10 A; A/B/C=20-479.
DR PDBsum; 5AZS; -.
DR AlphaFoldDB; Q51397; -.
DR SMR; Q51397; -.
DR STRING; 287.DR97_1903; -.
DR PaxDb; Q51397; -.
DR EnsemblBacteria; AAG07985; AAG07985; PA4597.
DR GeneID; 881070; -.
DR KEGG; pae:PA4597; -.
DR PATRIC; fig|208964.12.peg.4812; -.
DR PseudoCAP; PA4597; -.
DR HOGENOM; CLU_012817_13_3_6; -.
DR InParanoid; Q51397; -.
DR OMA; ILQDRNA; -.
DR PhylomeDB; Q51397; -.
DR BioCyc; PAER208964:G1FZ6-4691-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR InterPro; IPR003423; OMP_efflux.
DR InterPro; IPR010131; RND_efflux_OM_lipoprot_NodT.
DR Pfam; PF02321; OEP; 2.
DR TIGRFAMs; TIGR01845; outer_NodT; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antibiotic resistance; Cell outer membrane; Lipoprotein;
KW Membrane; Palmitate; Reference proteome; Signal; Transmembrane;
KW Transmembrane beta strand.
FT SIGNAL 1..19
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 20..479
FT /note="Outer membrane protein OprJ"
FT /id="PRO_0000030998"
FT REGION 102..121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 106..121
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 20
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000305"
FT LIPID 20
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000305"
FT CONFLICT 74
FT /note="N -> I (in Ref. 1; AAB41958)"
FT /evidence="ECO:0000305"
FT CONFLICT 339
FT /note="W -> C (in Ref. 1; AAB41958)"
FT /evidence="ECO:0000305"
FT HELIX 54..57
FT /evidence="ECO:0007829|PDB:5AZS"
FT HELIX 61..73
FT /evidence="ECO:0007829|PDB:5AZS"
FT HELIX 75..98
FT /evidence="ECO:0007829|PDB:5AZS"
FT STRAND 102..113
FT /evidence="ECO:0007829|PDB:5AZS"
FT TURN 115..117
FT /evidence="ECO:0007829|PDB:5AZS"
FT STRAND 118..122
FT /evidence="ECO:0007829|PDB:5AZS"
FT STRAND 124..140
FT /evidence="ECO:0007829|PDB:5AZS"
FT HELIX 145..212
FT /evidence="ECO:0007829|PDB:5AZS"
FT HELIX 217..252
FT /evidence="ECO:0007829|PDB:5AZS"
FT HELIX 257..260
FT /evidence="ECO:0007829|PDB:5AZS"
FT HELIX 280..286
FT /evidence="ECO:0007829|PDB:5AZS"
FT HELIX 288..311
FT /evidence="ECO:0007829|PDB:5AZS"
FT STRAND 314..327
FT /evidence="ECO:0007829|PDB:5AZS"
FT HELIX 328..330
FT /evidence="ECO:0007829|PDB:5AZS"
FT STRAND 331..333
FT /evidence="ECO:0007829|PDB:5AZS"
FT STRAND 337..350
FT /evidence="ECO:0007829|PDB:5AZS"
FT HELIX 355..421
FT /evidence="ECO:0007829|PDB:5AZS"
FT HELIX 426..461
FT /evidence="ECO:0007829|PDB:5AZS"
SQ SEQUENCE 479 AA; 51958 MW; 61322544800A4F36 CRC64;
MRKPAFGVSA LLIALTLGAC SMAPTYERPA APVADSWSGA AAQRQGAAID TLDWKSFIVD
AELRRLVDMA LDNNRSLRQT LLDIEAARAQ YRIQRADRVP GLNAAATGNR QRQPADLSAG
NRSEVASSYQ VGLALPEYEL DLFGRVKSLT DAALQQYLAS EEAARAARIA LVAEVSQAYL
SYDGALRRLA LTRQTLVSRE YSFALIDQRR AAGAATALDY QEALGLVEQA RAEQERNLRQ
KQQAFNALVL LLGSDDAAQA IPRSPGQRPK LLQDIAPGTP SELIERRPDI LAAEHRLRAR
NADIGAARAA FFPRISLTGS FGTSSAEMSG LFDGGSRSWS FLPTLTLPIF DGGRNRANLS
LAEARKDSAV AAYEGTIQTA FREVADALAA SDTLRREEKA LRALANSSNE ALKLAKARYE
SGVDNHLRYL DAQRSSFLNE IAFIDGSTQR QIALVDLFRA LGGGWDEGRS LVVHRGGRS
