ID   ARY_GALME               Reviewed;         702 AA.
AC   Q24995;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Arylphorin {ECO:0000303|Ref.1};
DE   AltName: Full=Lhp76 {ECO:0000303|Ref.1};
DE   Flags: Precursor;
GN   Name=LOC113516268 {ECO:0000312|RefSeq:XP_026756460.1};
GN   Synonyms=Arylphorin {ECO:0000303|Ref.1};
OS   Galleria mellonella (Greater wax moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Pyraloidea;
OC   Pyralidae; Galleriinae; Galleria.
OX   NCBI_TaxID=7137 {ECO:0000312|Proteomes:UP000504614};
RN   [1] {ECO:0000312|EMBL:AAA74229.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, AND DEVELOPMENTAL
RP   STAGE.
RA   Memmel N.A., Trewitt P.M., Silhacek D.E., Kumaran A.K.;
RT   "Nucleotide sequence and structure of the arylphorin gene from Galleria
RT   mellonella.";
RL   Insect Biochem. Mol. Biol. 22:333-342(1992).
RN   [2] {ECO:0000305}
RP   IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBCELLULAR LOCATION, AND
RP   TISSUE SPECIFICITY.
RX   PubMed=34443685; DOI=10.3390/molecules26165097;
RA   Staczek S., Zdybicka-Barabas A., Wojda I., Wiater A., Mak P., Suder P.,
RA   Skrzypiec K., Cytrynska M.;
RT   "Fungal alpha-1,3-Glucan as a New Pathogen-Associated Molecular Pattern in
RT   the Insect Model Host Galleria mellonella.";
RL   Molecules 26:5097-5097(2021).
CC   -!- FUNCTION: Larval storage protein (LSP) which may serve as a store of
CC       amino acids for synthesis of adult proteins (Probable). Binds the
CC       A.niger cell wall component alpha-1,3-glucan, a fungal pathogen-
CC       associated molecular pattern (PAMP) that activates the host immune
CC       response (PubMed:34443685). {ECO:0000269|PubMed:34443685, ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:34443685,
CC       ECO:0000269|Ref.1}. Note=Secreted in the hemolymph.
CC       {ECO:0000269|PubMed:34443685, ECO:0000269|Ref.1}.
CC   -!- TISSUE SPECIFICITY: Hemolymph. {ECO:0000269|PubMed:34443685}.
CC   -!- DEVELOPMENTAL STAGE: In last instar larval fat body and hemolymph.
CC       {ECO:0000269|Ref.1}.
CC   -!- SIMILARITY: Belongs to the hemocyanin family. {ECO:0000305}.
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DR   EMBL; M73793; AAA74229.1; -; Genomic_DNA.
DR   PIR; A61619; A61619.
DR   RefSeq; XP_026756460.1; XM_026900659.1.
DR   SMR; Q24995; -.
DR   Proteomes; UP000504614; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1280.10; -; 1.
DR   Gene3D; 1.20.1370.10; -; 1.
DR   Gene3D; 2.60.40.1520; -; 1.
DR   InterPro; IPR008922; Di-copper_centre_dom_sf.
DR   InterPro; IPR013788; Hemocyanin/hexamerin.
DR   InterPro; IPR000896; Hemocyanin/hexamerin_mid_dom.
DR   InterPro; IPR005203; Hemocyanin_C.
DR   InterPro; IPR037020; Hemocyanin_C_sf.
DR   InterPro; IPR005204; Hemocyanin_N.
DR   InterPro; IPR036697; Hemocyanin_N_sf.
DR   InterPro; IPR014756; Ig_E-set.
DR   PANTHER; PTHR11511; PTHR11511; 1.
DR   Pfam; PF03723; Hemocyanin_C; 1.
DR   Pfam; PF00372; Hemocyanin_M; 1.
DR   Pfam; PF03722; Hemocyanin_N; 1.
DR   PRINTS; PR00187; HAEMOCYANIN.
DR   SUPFAM; SSF48050; SSF48050; 1.
DR   SUPFAM; SSF48056; SSF48056; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
DR   PROSITE; PS00209; HEMOCYANIN_1; 1.
DR   PROSITE; PS00210; HEMOCYANIN_2; 1.
PE   1: Evidence at protein level;
KW   Glycoprotein; Reference proteome; Secreted; Signal; Storage protein.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255"
FT   CHAIN           17..702
FT                   /note="Arylphorin"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5029440263"
FT   CARBOHYD        211
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        481
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   702 AA;  83704 MW;  E7590F729DC084EA CRC64;
     MQTVLFLAAL VSLAAAGYPQ YHYDVETRKL DPSLLNIQTK VLSLLENWKQ VNPDDEYYKI
     GKEYNVEANM ESYTNREVVT EFLSLYKAGF IPKNEVFSIF YENQALEVIA LYRLFYYAKD
     FETFYKTAAF ARVWLNEGQF VYAFYLAVIH RADTRGIVLP APYEIWPEYF MNSDVLSKIY
     RIQMQKGLII PEQGPYYGIL SKDNAYYFYA NYSGPLTYED NENLLSYFIE DIGWNSYYYY
     FHNRFPFWEN GEQLIGPLKE RRGEIYYYVY QKILARYYLE RLANGLGEIP RFNWLDKYQT
     SYYPLLSSYQ LPFAQRNDDY YLASGDNIND IQFIDTYEKT FLQLLQKGQF KAYKQEVDLY
     NSKSINFVGN YWQSNADLYE KVPKRNYWRS YEATARRVLG AAPRSSINYE NMNIPTALDF
     YQTSLRDPAF YQLYAKILDY INEYKEYLEP YSQDVLHYVG VKINDVKVDK LVTYFEYFDW
     NATNAVYLSE QQLDTVSPSY IVRQPRLNNK PFTVNIDIKS DVESEVVVKI FLGPKYDGNG
     LPISLEDNWI NFIELDWFTH KLTSGQNKIA RKSEEFFFFK DDSVSLFKIY ELLSNGQVPS
     YMVDRYIYLP RRLILPRGTQ RGFPLQLFVV VYPYQAPVKE WESMRQYIVD NKPFGYPFDR
     PVTLPYYFNQ PNMYFKDVYV YQEGEQYPYY NSYWSQNQVS NH