OPS3_DROME
ID OPS3_DROME Reviewed; 383 AA.
AC P04950; Q9TX53;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 03-AUG-2022, entry version 188.
DE RecName: Full=Opsin Rh3;
DE AltName: Full=Inner R7 photoreceptor cells opsin;
GN Name=Rh3; Synonyms=RH92CD; ORFNames=CG10888;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Canton-S;
RX PubMed=2953598; DOI=10.1002/j.1460-2075.1987.tb04774.x;
RA Fryxell K.J., Meyerowitz E.M.;
RT "An opsin gene that is expressed only in the R7 photoreceptor cell of
RT Drosophila.";
RL EMBO J. 6:443-451(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2437266; DOI=10.1523/jneurosci.07-05-01550.1987;
RA Zuker C.S., Montell C., Jones K., Laverty T., Rubin G.M.;
RT "A rhodopsin gene expressed in photoreceptor cell R7 of the Drosophila eye:
RT homologies with other signal-transducing molecules.";
RL J. Neurosci. 7:1550-1557(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=10731138; DOI=10.1126/science.287.5461.2222;
RA Rubin G.M., Hong L., Brokstein P., Evans-Holm M., Frise E., Stapleton M.,
RA Harvey D.A.;
RT "A Drosophila complementary DNA resource.";
RL Science 287:2222-2224(2000).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 34-355.
RX PubMed=8006992; DOI=10.1007/bf00176087;
RA Carulli J.P., Chen D.M., Stark W.S., Hartl D.L.;
RT "Phylogeny and physiology of Drosophila opsins.";
RL J. Mol. Evol. 38:250-262(1994).
CC -!- FUNCTION: Visual pigments are the light-absorbing molecules that
CC mediate vision. They consist of an apoprotein, opsin, covalently linked
CC to cis-retinal.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- PTM: Phosphorylated on some or all of the serine and threonine residues
CC present in the C-terminal region.
CC -!- MISCELLANEOUS: Each Drosophila eye is composed of 800 facets or
CC ommatidia. Each ommatidium contains 8 photoreceptor cells (R1-R8), the
CC R1 to R6 cells are outer cells, while R7 and R8 are inner cells.
CC -!- MISCELLANEOUS: Opsin Rh3 is sensitive to UV light.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Opsin
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; Y00043; CAA68259.1; -; Genomic_DNA.
DR EMBL; M17718; AAA28854.1; -; Genomic_DNA.
DR EMBL; AE014297; AAG22157.1; -; Genomic_DNA.
DR EMBL; AF184224; AAD55735.1; -; mRNA.
DR PIR; A26768; A26768.
DR RefSeq; NP_524411.1; NM_079687.3.
DR AlphaFoldDB; P04950; -.
DR SMR; P04950; -.
DR BioGRID; 67383; 2.
DR IntAct; P04950; 1.
DR STRING; 7227.FBpp0083284; -.
DR GlyGen; P04950; 1 site.
DR PaxDb; P04950; -.
DR PRIDE; P04950; -.
DR DNASU; 42398; -.
DR EnsemblMetazoa; FBtr0083876; FBpp0083284; FBgn0003249.
DR GeneID; 42398; -.
DR KEGG; dme:Dmel_CG10888; -.
DR CTD; 42398; -.
DR FlyBase; FBgn0003249; Rh3.
DR VEuPathDB; VectorBase:FBgn0003249; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01030000234631; -.
DR HOGENOM; CLU_009579_3_0_1; -.
DR InParanoid; P04950; -.
DR OMA; VACYTES; -.
DR OrthoDB; 704940at2759; -.
DR PhylomeDB; P04950; -.
DR Reactome; R-DME-416476; G alpha (q) signalling events.
DR Reactome; R-DME-419771; Opsins.
DR SignaLink; P04950; -.
DR BioGRID-ORCS; 42398; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Rh3; fly.
DR GenomeRNAi; 42398; -.
DR PRO; PR:P04950; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0003249; Expressed in head capsule and 9 other tissues.
DR Genevisible; P04950; DM.
DR GO; GO:0016021; C:integral component of membrane; ISS:FlyBase.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0008020; F:G protein-coupled photoreceptor activity; IDA:FlyBase.
DR GO; GO:0016039; P:absorption of UV light; IMP:FlyBase.
DR GO; GO:0071482; P:cellular response to light stimulus; IBA:GO_Central.
DR GO; GO:0009589; P:detection of UV; TAS:FlyBase.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISS:FlyBase.
DR GO; GO:0007602; P:phototransduction; IBA:GO_Central.
DR GO; GO:0007604; P:phototransduction, UV; IDA:FlyBase.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR000856; Opsin_RH3/RH4.
DR InterPro; IPR027430; Retinal_BS.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00577; OPSINRH3RH4.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR PROSITE; PS00238; OPSIN; 1.
PE 1: Evidence at protein level;
KW Chromophore; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Phosphoprotein; Photoreceptor protein; Receptor;
KW Reference proteome; Retinal protein; Sensory transduction; Transducer;
KW Transmembrane; Transmembrane helix; Vision.
FT CHAIN 1..383
FT /note="Opsin Rh3"
FT /id="PRO_0000197627"
FT TOPO_DOM 1..57
FT /note="Extracellular"
FT TRANSMEM 58..82
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..94
FT /note="Cytoplasmic"
FT TRANSMEM 95..119
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 120..133
FT /note="Extracellular"
FT TRANSMEM 134..153
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 154..171
FT /note="Cytoplasmic"
FT TRANSMEM 172..196
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..220
FT /note="Extracellular"
FT TRANSMEM 221..248
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 249..284
FT /note="Cytoplasmic"
FT TRANSMEM 285..308
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 309..316
FT /note="Extracellular"
FT TRANSMEM 317..341
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 342..383
FT /note="Cytoplasmic"
FT REGION 362..383
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 364..383
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 328
FT /note="N6-(retinylidene)lysine"
FT CARBOHYD 13
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000305"
FT DISULFID 130..207
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 383 AA; 42940 MW; BF9D009A25CA6343 CRC64;
MESGNVSSSL FGNVSTALRP EARLSAETRL LGWNVPPEEL RHIPEHWLTY PEPPESMNYL
LGTLYIFFTL MSMLGNGLVI WVFSAAKSLR TPSNILVINL AFCDFMMMVK TPIFIYNSFH
QGYALGHLGC QIFGIIGSYT GIAAGATNAF IAYDRFNVIT RPMEGKMTHG KAIAMIIFIY
MYATPWVVAC YTETWGRFVP EGYLTSCTFD YLTDNFDTRL FVACIFFFSF VCPTTMITYY
YSQIVGHVFS HEKALRDQAK KMNVESLRSN VDKNKETAEI RIAKAAITIC FLFFCSWTPY
GVMSLIGAFG DKTLLTPGAT MIPACACKMV ACIDPFVYAI SHPRYRMELQ KRCPWLALNE
KAPESSAVAS TSTTQEPQQT TAA
