OPS4_DROME
ID OPS4_DROME Reviewed; 378 AA.
AC P08255; Q9VVB3;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=Opsin Rh4;
DE AltName: Full=Inner R7 photoreceptor cells opsin;
GN Name=Rh4; ORFNames=CG9668;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2952772; DOI=10.1523/jneurosci.07-05-01558.1987;
RA Montell C., Jones K., Zuker C.S., Rubin G.M.;
RT "A second opsin gene expressed in the ultraviolet-sensitive R7
RT photoreceptor cells of Drosophila melanogaster.";
RL J. Neurosci. 7:1558-1566(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Visual pigments are the light-absorbing molecules that
CC mediate vision. They consist of an apoprotein, opsin, covalently linked
CC to cis-retinal.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- PTM: Phosphorylated on some or all of the serine and threonine residues
CC present in the C-terminal region.
CC -!- MISCELLANEOUS: Each Drosophila eye is composed of 800 facets or
CC ommatidia. Each ommatidium contains 8 photoreceptor cells (R1-R8), the
CC R1 to R6 cells are outer cells, while R7 and R8 are inner cells.
CC -!- MISCELLANEOUS: Opsin Rh4 is sensitive to UV light.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Opsin
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; M17730; AAA28856.1; -; Genomic_DNA.
DR EMBL; M17719; AAA28856.1; JOINED; Genomic_DNA.
DR EMBL; AE014296; AAF49400.1; -; Genomic_DNA.
DR PIR; S28789; S28789.
DR RefSeq; NP_476701.1; NM_057353.4.
DR AlphaFoldDB; P08255; -.
DR SMR; P08255; -.
DR BioGRID; 65186; 1.
DR STRING; 7227.FBpp0075097; -.
DR GlyGen; P08255; 1 site.
DR PaxDb; P08255; -.
DR DNASU; 39887; -.
DR EnsemblMetazoa; FBtr0075338; FBpp0075097; FBgn0003250.
DR GeneID; 39887; -.
DR KEGG; dme:Dmel_CG9668; -.
DR CTD; 39887; -.
DR FlyBase; FBgn0003250; Rh4.
DR VEuPathDB; VectorBase:FBgn0003250; -.
DR eggNOG; KOG3656; Eukaryota.
DR HOGENOM; CLU_009579_3_0_1; -.
DR InParanoid; P08255; -.
DR OMA; GCQIFAA; -.
DR OrthoDB; 704940at2759; -.
DR PhylomeDB; P08255; -.
DR BioGRID-ORCS; 39887; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Rh4; fly.
DR GenomeRNAi; 39887; -.
DR PRO; PR:P08255; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0003250; Expressed in head capsule and 13 other tissues.
DR ExpressionAtlas; P08255; baseline and differential.
DR Genevisible; P08255; DM.
DR GO; GO:0016021; C:integral component of membrane; ISS:FlyBase.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0008020; F:G protein-coupled photoreceptor activity; IDA:FlyBase.
DR GO; GO:0071482; P:cellular response to light stimulus; IBA:GO_Central.
DR GO; GO:0009589; P:detection of UV; TAS:FlyBase.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISS:FlyBase.
DR GO; GO:0007602; P:phototransduction; IBA:GO_Central.
DR GO; GO:0007604; P:phototransduction, UV; IDA:FlyBase.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR000856; Opsin_RH3/RH4.
DR InterPro; IPR027430; Retinal_BS.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00577; OPSINRH3RH4.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR PROSITE; PS00238; OPSIN; 1.
PE 1: Evidence at protein level;
KW Chromophore; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Phosphoprotein; Photoreceptor protein; Receptor;
KW Reference proteome; Retinal protein; Sensory transduction; Transducer;
KW Transmembrane; Transmembrane helix; Vision.
FT CHAIN 1..378
FT /note="Opsin Rh4"
FT /id="PRO_0000197630"
FT TOPO_DOM 1..53
FT /note="Extracellular"
FT TRANSMEM 54..78
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 79..90
FT /note="Cytoplasmic"
FT TRANSMEM 91..111
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..127
FT /note="Extracellular"
FT TRANSMEM 128..148
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 149..167
FT /note="Cytoplasmic"
FT TRANSMEM 168..192
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 193..216
FT /note="Extracellular"
FT TRANSMEM 217..244
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 245..280
FT /note="Cytoplasmic"
FT TRANSMEM 281..304
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 305..312
FT /note="Extracellular"
FT TRANSMEM 313..337
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 338..378
FT /note="Cytoplasmic"
FT MOD_RES 324
FT /note="N6-(retinylidene)lysine"
FT CARBOHYD 6
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000305"
FT DISULFID 126..203
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 111..112
FT /note="Missing (in Ref. 1; AAA28856)"
FT /evidence="ECO:0000305"
FT CONFLICT 120
FT /note="A -> AIY (in Ref. 1; AAA28856)"
FT /evidence="ECO:0000305"
FT CONFLICT 313
FT /note="P -> Q (in Ref. 1; AAA28856)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 378 AA; 42658 MW; 9A33C1E6F7BD11AD CRC64;
MEPLCNASEP PLRPEARSSG NGDLQFLGWN VPPDQIQYIP EHWLTQLEPP ASMHYMLGVF
YIFLFCASTV GNGMVIWIFS TSKSLRTPSN MFVLNLAVFD LIMCLKAPIF IYNSFHRGFA
LGNTWCQIFA SIGSYSGIGA GMTNAAIGYD RYNVITKPMN RNMTFTKAVI MNIIIWLYCT
PWVVLPLTQF WDRFVPEGYL TSCSFDYLSD NFDTRLFVGT IFFFSFVCPT LMILYYYSQI
VGHVFSHEKA LREQAKKMNV ESLRSNVDKS KETAEIRIAK AAITICFLFF VSWTPYGVMS
LIGAFGDKSL LTPGATMIPA CTCKLVACID PFVYAISHPR YRLELQKRCP WLGVNEKSGE
ISSAQSTTTQ EQQQTTAA
