ID   OPS7_BEAB2              Reviewed;         305 AA.
AC   J5J930;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2012, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Oxidoreductase OpS7 {ECO:0000303|PubMed:26305932};
DE            EC=1.-.-.- {ECO:0000269|PubMed:26305932};
DE   AltName: Full=Oosporein biosynthesis protein 7 {ECO:0000303|PubMed:26305932};
GN   Name=OpS7 {ECO:0000303|PubMed:26305932}; ORFNames=BBA_08185;
OS   Beauveria bassiana (strain ARSEF 2860) (White muscardine disease fungus)
OS   (Tritirachium shiotae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Cordycipitaceae; Beauveria.
OX   NCBI_TaxID=655819;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ARSEF 2860;
RX   PubMed=22761991; DOI=10.1038/srep00483;
RA   Xiao G., Ying S.-H., Zheng P., Wang Z.-L., Zhang S., Xie X.-Q., Shang Y.,
RA   St Leger R.J., Zhao G.-P., Wang C., Feng M.-G.;
RT   "Genomic perspectives on the evolution of fungal entomopathogenicity in
RT   Beauveria bassiana.";
RL   Sci. Rep. 2:483-483(2012).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, INDUCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX   PubMed=26305932; DOI=10.1073/pnas.1503200112;
RA   Feng P., Shang Y., Cen K., Wang C.;
RT   "Fungal biosynthesis of the bibenzoquinone oosporein to evade insect
RT   immunity.";
RL   Proc. Natl. Acad. Sci. U.S.A. 112:11365-11370(2015).
CC   -!- FUNCTION: Oxidoreductase; part of the gene cluster that mediates the
CC       biosynthesis of the bibenzoquinone oosporein, a metabolite required for
CC       fungal virulence that acts by evading host immunity to facilitate
CC       fungal multiplication in insects (PubMed:26305932). The non-reducing
CC       polyketide synthase OpS1 produces orsellinic acid by condensing acetyl-
CC       CoA with 3 malonyl-CoA units (PubMed:26305932). Orsellinic acid is then
CC       hydroxylated to benzenetriol by the hydroxylase OpS4 (PubMed:26305932).
CC       The intermediate is oxidized either nonenzymatically to 5,5'-dideoxy-
CC       oosporein or enzymatically to benzenetetrol by the oxidoreductase OpS7
CC       (PubMed:26305932). The latter is further dimerized to oosporein by the
CC       catalase OpS5 (PubMed:26305932). OpS6 probably functions en route for
CC       protecting cells against oxidative stress by scavenging any leaked free
CC       radical form of benzenetetrol by activating the thiol group of
CC       glutathione (PubMed:26305932). {ECO:0000269|PubMed:26305932}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:26305932}.
CC   -!- INDUCTION: Expression is positively regulated by the oosporein cluster
CC       specific regulator OpS3 that binds the promoter at a 5'-CGGA-3' motif
CC       (PubMed:26305932). Expression is negatively regulated by the global
CC       transcription factor Msn2 that binds the stress-response element 5'-
CC       AGGGG-3' (PubMed:26305932). {ECO:0000269|PubMed:26305932}.
CC   -!- DISRUPTION PHENOTYPE: Impairs the conversion of 5,5'-dideoxy-oosporein
CC       into oosporein (PubMed:26305932). {ECO:0000269|PubMed:26305932}.
CC   -!- SIMILARITY: Belongs to the oxidoreductase OpS7 family. {ECO:0000305}.
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DR   EMBL; JH725181; EJP62798.1; -; Genomic_DNA.
DR   RefSeq; XP_008601504.1; XM_008603282.1.
DR   AlphaFoldDB; J5J930; -.
DR   PRIDE; J5J930; -.
DR   EnsemblFungi; EJP62798; EJP62798; BBA_08185.
DR   GeneID; 19891197; -.
DR   HOGENOM; CLU_068080_0_0_1; -.
DR   InParanoid; J5J930; -.
DR   Proteomes; UP000002762; Unassembled WGS sequence.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   SUPFAM; SSF51182; SSF51182; 1.
PE   1: Evidence at protein level;
KW   Oxidoreductase; Reference proteome; Virulence.
FT   CHAIN           1..305
FT                   /note="Oxidoreductase OpS7"
FT                   /id="PRO_0000438582"
SQ   SEQUENCE   305 AA;  34292 MW;  730DA2F3F3436108 CRC64;
     MGFVPITLSA GQNPLTQFDG KIETHFLEPP AGTAFQIMQI YKPLPKGDKS GAYRGPPPHF
     HLHQTERFKV IKGRVGIEVN DKVTVLRPKD GVAICPAGNI HRFIIDVDPK HDQDGEDDEE
     EDDGEIVFMV NATDSGKDFV LDRIFLENWY GVRVDSFKYG TKIDFIQQCA TFDGGDHYLP
     FPATLPEWVP MSWSVAIRTF LGFWVTVIIG RYVGGLLGYQ PFYREYTTDW ELAVAKMQGT
     WFYRRNVQTA YRAATSWKEL REMVPYSDEG AANMGLADAK VGNGAAVKKA INDRAANNGE
     DKKNL