OPSB_APIME
ID OPSB_APIME Reviewed; 377 AA.
AC P90680; O61302; Q2YD69;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 2.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Opsin, blue-sensitive;
DE AltName: Full=Blue-sensitive opsin;
DE Short=AmBLop;
GN Name=BLOP;
OS Apis mellifera (Honeybee).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC Apis.
OX NCBI_TaxID=7460;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Head;
RX PubMed=9057845; DOI=10.1111/j.1432-1033.1997.00775.x;
RA Bellingham J., Wilkie S.E., Morris A.G., Bowmaker J.K., Hunt D.M.;
RT "Characterisation of the ultraviolet-sensitive opsin gene in the honey bee,
RT Apis mellifera.";
RL Eur. J. Biochem. 243:775-781(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Head;
RX PubMed=9502802; DOI=10.1523/jneurosci.18-07-02412.1998;
RA Townson S.M., Chang B.S.W., Salcedo E., Chadwell L.V., Pierce N.E.,
RA Britt S.G.;
RT "Honeybee blue- and ultraviolet-sensitive opsins: cloning, heterologous
RT expression in Drosophila, and physiological characterization.";
RL J. Neurosci. 18:2412-2422(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DH4;
RG Honey bee genome project;
RA Zhang L., Deng J., Wu Y.-Q., Kovar C., Aqrawi P., Bandaranaike D.,
RA Blankenburg K., Chen D., Denson S., Dinh H., Firestine M., Gross S.,
RA Han Y., Hernandez B., Holder M., Jackson L., Javaid M., Jing C., Jones J.,
RA Joshi V., Kamau G., Korchina V., Lee S., Lorensuhewa L., Mata R.,
RA Mathew T., Mims S., Ngo R., Nguyen L., Okwuonu G., Ongeri F., Osuji N.,
RA Pham C., Puazo M., Qu C., Quiroz J., Raj R., Rio Deiros D., Santibanez J.,
RA Scheel M., Scherer S., Vee V., Wang M., Xin Y., Richards S., Reid J.G.,
RA Newsham I., Worley K.C., Muzny D.M., Gibbs R.;
RL Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=16291092; DOI=10.1016/j.ibmb.2005.09.001;
RA Velarde R.A., Sauer C.D., Walden K.K.O., Fahrbach S.E., Robertson H.M.;
RT "Pteropsin: a vertebrate-like non-visual opsin expressed in the honey bee
RT brain.";
RL Insect Biochem. Mol. Biol. 35:1367-1377(2005).
CC -!- FUNCTION: Visual pigments are the light-absorbing molecules that
CC mediate vision. They consist of an apoprotein, opsin, covalently linked
CC to 11-cis-retinal.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Absorption:
CC Abs(max)=439 nm;
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in the dorsal region of the retina and
CC sparsely expressed in the ventral region.
CC {ECO:0000269|PubMed:16291092}.
CC -!- PTM: Phosphorylated on some or all of the serine and threonine residues
CC present in the C-terminal region. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Opsin
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC -!- CAUTION: Was originally thought to be the ultraviolet sensitive opsin.
CC {ECO:0000305|PubMed:9057845}.
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DR EMBL; U70841; AAC47455.1; -; mRNA.
DR EMBL; AF004168; AAC13417.1; -; mRNA.
DR EMBL; AADG06002981; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BK005512; DAA05737.1; -; Genomic_DNA.
DR RefSeq; NP_001011606.1; NM_001011606.1.
DR AlphaFoldDB; P90680; -.
DR SMR; P90680; -.
DR STRING; 7460.GB41643-PA; -.
DR PaxDb; P90680; -.
DR EnsemblMetazoa; NM_001011606; NP_001011606; GeneID_406128.
DR GeneID; 406128; -.
DR KEGG; ame:406128; -.
DR CTD; 406128; -.
DR eggNOG; KOG3656; Eukaryota.
DR HOGENOM; CLU_009579_3_0_1; -.
DR InParanoid; P90680; -.
DR OMA; MLACKSV; -.
DR OrthoDB; 723541at2759; -.
DR PhylomeDB; P90680; -.
DR Proteomes; UP000005203; Unplaced.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0009881; F:photoreceptor activity; IEA:UniProtKB-KW.
DR GO; GO:0050908; P:detection of light stimulus involved in visual perception; IDA:CACAO.
DR GO; GO:0007602; P:phototransduction; IEA:UniProtKB-KW.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR001760; Opsin.
DR InterPro; IPR000856; Opsin_RH3/RH4.
DR InterPro; IPR027430; Retinal_BS.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00238; OPSIN.
DR PRINTS; PR00577; OPSINRH3RH4.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR PROSITE; PS00238; OPSIN; 1.
PE 1: Evidence at protein level;
KW Chromophore; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Phosphoprotein; Photoreceptor protein; Receptor;
KW Reference proteome; Retinal protein; Sensory transduction; Transducer;
KW Transmembrane; Transmembrane helix; Vision.
FT CHAIN 1..377
FT /note="Opsin, blue-sensitive"
FT /id="PRO_0000197638"
FT TOPO_DOM 1..56
FT /note="Extracellular"
FT TRANSMEM 57..81
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 82..93
FT /note="Cytoplasmic"
FT TRANSMEM 94..119
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 120..132
FT /note="Extracellular"
FT TRANSMEM 133..152
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 153..170
FT /note="Cytoplasmic"
FT TRANSMEM 171..195
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 196..219
FT /note="Extracellular"
FT TRANSMEM 220..247
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 248..282
FT /note="Cytoplasmic"
FT TRANSMEM 283..306
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 307..314
FT /note="Extracellular"
FT TRANSMEM 315..339
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 340..377
FT /note="Cytoplasmic"
FT MOD_RES 326
FT /note="N6-(retinylidene)lysine"
FT /evidence="ECO:0000250"
FT CARBOHYD 5
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 129..206
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 43
FT /note="H -> R (in Ref. 1; AAC47455)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 377 AA; 42997 MW; 5329E0593C3492E6 CRC64;
MLLHNKTLAG KALAFIAEEG YVPSMREKFL GWNVPPEYSD LVHPHWRAFP APGKHFHIGL
AIIYSMLLIM SLVGNCCVIW IFSTSKSLRT PSNMFIVSLA IFDIIMAFEM PMLVISSFME
RMIGWEIGCD VYSVFGSISG MGQAMTNAAI AFDRYRTISC PIDGRLNSKQ AAVIIAFTWF
WVTPFTVLPL LKVWGRYTTE GFLTTCSFDF LTDDEDTKVF VTCIFIWAYV IPLIFIILFY
SRLLSSIRNH EKMLREQAKK MNVKSLVSNQ DKERSAEVRI AKVAFTIFFL FLLAWTPYAT
VALIGVYGNR ELLTPVSTML PAVFAKTVSC IDPWIYAINH PRYRQELQKR CKWMGIHEPE
TTSDATSAQT EKIKTDE
