OPSB_MOUSE
ID OPSB_MOUSE Reviewed; 346 AA.
AC P51491; Q548Z8;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Short-wave-sensitive opsin 1;
DE Short=S opsin;
DE AltName: Full=Blue cone photoreceptor pigment;
DE AltName: Full=Blue-sensitive opsin;
DE Short=BOP;
DE AltName: Full=Short wavelength-sensitive cone opsin;
GN Name=Opn1sw; Synonyms=Bcp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC TISSUE=Retina;
RX PubMed=8088841; DOI=10.1006/geno.1994.1292;
RA Chiu M.I., Zack D.J., Wang Y., Nathans J.;
RT "Murine and bovine blue cone pigment genes: cloning and characterization of
RT two new members of the S family of visual pigments.";
RL Genomics 21:440-443(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=BALB/cJ, and C57BL/10;
RX PubMed=11055434; DOI=10.1016/s0896-6273(00)00062-3;
RA Applebury M.L., Antoch M.P., Baxter L.C., Chun L.Y., Falk J.D.,
RA Farhangfar F., Kage K., Krzystolik M.G., Lyass L.A., Robbins J.T.;
RT "The murine cone photoreceptor: a single cone type expresses both S and M
RT opsins with retinal spatial patterning.";
RL Neuron 27:513-523(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Retina;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=21219924; DOI=10.1016/j.visres.2010.12.017;
RA Daniele L.L., Insinna C., Chance R., Wang J., Nikonov S.S., Pugh E.N. Jr.;
RT "A mouse M-opsin monochromat: retinal cone photoreceptors have increased M-
RT opsin expression when S-opsin is knocked out.";
RL Vision Res. 51:447-458(2011).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=25416279; DOI=10.1093/hmg/ddu588;
RA Zhang T., Enemchukwu N.O., Jones A., Wang S., Dennis E., Watt C.B.,
RA Pugh E.N. Jr., Fu Y.;
RT "Genetic deletion of S-opsin prevents rapid cone degeneration in a mouse
RT model of Leber congenital amaurosis.";
RL Hum. Mol. Genet. 24:1755-1763(2015).
CC -!- FUNCTION: Visual pigments are the light-absorbing molecules that
CC mediate vision. They consist of an apoprotein, opsin, covalently linked
CC to cis-retinal (By similarity). Required for the maintenance of cone
CC outer segment organization in the ventral retina, but not essential for
CC the maintenance of functioning cone photoreceptors (PubMed:21219924,
CC PubMed:25416279). Involved in ensuring correct abundance and
CC localization of retinal membrane proteins (PubMed:25416279). May
CC increase spectral sensitivity in dim light (PubMed:11055434).
CC {ECO:0000250|UniProtKB:P03999, ECO:0000269|PubMed:11055434,
CC ECO:0000269|PubMed:21219924, ECO:0000269|PubMed:25416279}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P03999};
CC Multi-pass membrane protein {ECO:0000255}. Photoreceptor inner segment
CC {ECO:0000269|PubMed:21219924}. Cell projection, cilium, photoreceptor
CC outer segment {ECO:0000269|PubMed:21219924}. Cytoplasm, perinuclear
CC region {ECO:0000250|UniProtKB:P03999}.
CC -!- TISSUE SPECIFICITY: Expressed in the inner and outer segments of cone
CC photoreceptor cells in the retina (at protein level).
CC {ECO:0000269|PubMed:11055434, ECO:0000269|PubMed:21219924,
CC ECO:0000269|PubMed:25416279}.
CC -!- PTM: Phosphorylated on some or all of the serine and threonine residues
CC present in the C-terminal region.
CC -!- DISRUPTION PHENOTYPE: Knockout mice are viable with normal gross
CC morphology (PubMed:21219924). Cone photoreceptors are normal and
CC provide a normal flash response, however cone cells in the ventral
CC retina have thin or undetectable outer segments that exhibit mild to
CC severe disordered organization (PubMed:21219924, PubMed:25416279). Cone
CC cells show increased sensitivity to mid-wavelength light, potentially
CC as result of increased protein Opn1mw abundance (PubMed:21219924). Lrat
CC and Opn1sw double knockout mice show no change in cone cell viability
CC in the central and ventral retina, however show shorter and swollen
CC outer and inner segments at twelve months of age (PubMed:25416279).
CC Double knockout of Lrat and Opn1sw results in a reduction in the slow
CC degeneration of dorsal cone photoreceptors compared to Lrat knockout
CC mice (PubMed:25416279). Lrat and Opn1sw double knockout mice show a
CC reduced abundance of transmembrane and peripheral membrane-associated
CC proteins in cone inner and outer segments (PubMed:25416279).
CC {ECO:0000269|PubMed:21219924, ECO:0000269|PubMed:25416279}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Opsin
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; U49720; AAA92458.1; -; mRNA.
DR EMBL; U92562; AAB53320.1; -; Genomic_DNA.
DR EMBL; U92558; AAB53320.1; JOINED; Genomic_DNA.
DR EMBL; U92559; AAB53320.1; JOINED; Genomic_DNA.
DR EMBL; U92560; AAB53320.1; JOINED; Genomic_DNA.
DR EMBL; U92561; AAB53320.1; JOINED; Genomic_DNA.
DR EMBL; AF190670; AAG17989.1; -; mRNA.
DR EMBL; AF190671; AAG17990.1; -; Genomic_DNA.
DR EMBL; BC026021; AAH26021.1; -; mRNA.
DR EMBL; BC058267; AAH58267.1; -; mRNA.
DR CCDS; CCDS19959.1; -.
DR PIR; B54679; B54679.
DR RefSeq; NP_031564.1; NM_007538.3.
DR AlphaFoldDB; P51491; -.
DR SMR; P51491; -.
DR STRING; 10090.ENSMUSP00000079289; -.
DR GlyGen; P51491; 1 site.
DR PhosphoSitePlus; P51491; -.
DR PaxDb; P51491; -.
DR PRIDE; P51491; -.
DR ProteomicsDB; 294393; -.
DR Antibodypedia; 31899; 81 antibodies from 19 providers.
DR DNASU; 12057; -.
DR Ensembl; ENSMUST00000080428; ENSMUSP00000079289; ENSMUSG00000058831.
DR GeneID; 12057; -.
DR KEGG; mmu:12057; -.
DR UCSC; uc009bdi.2; mouse.
DR CTD; 611; -.
DR MGI; MGI:99438; Opn1sw.
DR VEuPathDB; HostDB:ENSMUSG00000058831; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01030000234549; -.
DR HOGENOM; CLU_009579_3_0_1; -.
DR InParanoid; P51491; -.
DR OMA; CLCYVPY; -.
DR OrthoDB; 940057at2759; -.
DR PhylomeDB; P51491; -.
DR TreeFam; TF324998; -.
DR Reactome; R-MMU-2187335; The retinoid cycle in cones (daylight vision).
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR Reactome; R-MMU-419771; Opsins.
DR BioGRID-ORCS; 12057; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Opn1sw; mouse.
DR PRO; PR:P51491; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; P51491; protein.
DR Bgee; ENSMUSG00000058831; Expressed in retinal neural layer and 34 other tissues.
DR ExpressionAtlas; P51491; baseline and differential.
DR Genevisible; P51491; MM.
DR GO; GO:0044297; C:cell body; ISO:MGI.
DR GO; GO:0120199; C:cone photoreceptor outer segment; IDA:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
DR GO; GO:0001917; C:photoreceptor inner segment; IEA:UniProtKB-SubCell.
DR GO; GO:0001750; C:photoreceptor outer segment; IDA:MGI.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0043195; C:terminal bouton; ISO:MGI.
DR GO; GO:0008020; F:G protein-coupled photoreceptor activity; IBA:GO_Central.
DR GO; GO:0071482; P:cellular response to light stimulus; IBA:GO_Central.
DR GO; GO:0071492; P:cellular response to UV-A; ISS:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007602; P:phototransduction; IBA:GO_Central.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR001760; Opsin.
DR InterPro; IPR001521; Opsin_blue.
DR InterPro; IPR027430; Retinal_BS.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00238; OPSIN.
DR PRINTS; PR00574; OPSINBLUE.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR PROSITE; PS00238; OPSIN; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cell projection; Chromophore; Cytoplasm; Disulfide bond;
KW G-protein coupled receptor; Glycoprotein; Membrane; Phosphoprotein;
KW Photoreceptor protein; Receptor; Reference proteome; Retinal protein;
KW Sensory transduction; Transducer; Transmembrane; Transmembrane helix;
KW Vision.
FT CHAIN 1..346
FT /note="Short-wave-sensitive opsin 1"
FT /id="PRO_0000197766"
FT TOPO_DOM 1..31
FT /note="Extracellular"
FT TRANSMEM 32..56
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 57..68
FT /note="Cytoplasmic"
FT TRANSMEM 69..94
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 95..108
FT /note="Extracellular"
FT TRANSMEM 109..128
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 129..147
FT /note="Cytoplasmic"
FT TRANSMEM 148..171
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 172..197
FT /note="Extracellular"
FT TRANSMEM 198..225
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 226..247
FT /note="Cytoplasmic"
FT TRANSMEM 248..271
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 272..279
FT /note="Extracellular"
FT TRANSMEM 280..304
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 305..346
FT /note="Cytoplasmic"
FT REGION 324..346
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 326..346
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 291
FT /note="N6-(retinylidene)lysine"
FT CARBOHYD 12
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000305"
FT DISULFID 105..182
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 346 AA; 38922 MW; 8BEEAB0198EE6863 CRC64;
MSGEDDFYLF QNISSVGPWD GPQYHLAPVW AFRLQAAFMG FVFFVGTPLN AIVLVATLHY
KKLRQPLNYI LVNVSLGGFL FCIFSVFTVF IASCHGYFLF GRHVCALEAF LGSVAGLVTG
WSLAFLAFER YVVICKPFGS IRFNSKHALM VVLATWIIGI GVSIPPFFGW SRFIPEGLQC
SCGPDWYTVG TKYRSEYYTW FLFIFCFIIP LSLICFSYSQ LLRTLRAVAA QQQESATTQK
AEREVSHMVV VMVGSFCLCY VPYAALAMYM VNNRNHGLDL RLVTIPAFFS KSSCVYNPII
YCFMNKQFRA CILEMVCRKP MADESDVSGS QKTEVSTVSS SKVGPH
