ID   AS1C_DOLGE              Reviewed;          80 AA.
AC   A0A3G5BIC2;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   13-FEB-2019, sequence version 1.
DT   25-MAY-2022, entry version 10.
DE   RecName: Full=U-Asilidin(1)-Dg12 {ECO:0000303|PubMed:30400621};
DE   Flags: Precursor;
OS   Dolopus genitalis (Giant Australian assassin fly) (Asilus genitalis).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Asiloidea;
OC   Asilidae; Asilinae; Dolopus.
OX   NCBI_TaxID=2488630;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=30400621; DOI=10.3390/toxins10110456;
RA   Walker A.A., Dobson J., Jin J., Robinson S.D., Herzig V., Vetter I.,
RA   King G.F., Fry B.G.;
RT   "Buzz kill: function and proteomic composition of venom from the giant
RT   assassin fly Dolopus genitalis (Diptera: Asilidae).";
RL   Toxins 10:E456-E456(2018).
RN   [2] {ECO:0007744|PDB:6PX7}
RP   STRUCTURE BY NMR OF 47-80, FUNCTION, DISULFIDE BONDS, RECOMBINANT
RP   EXPRESSION, MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=31870846; DOI=10.1016/j.ibmb.2019.103310;
RA   Jin J., Agwa A.J., Szanto T.G., Csoti A., Panyi G., Schroeder C.I.,
RA   Walker A.A., King G.F.;
RT   "Weaponisation 'on the fly': convergent recruitment of knottin and defensin
RT   peptide scaffolds into the venom of predatory assassin flies.";
RL   Insect Biochem. Mol. Biol. 118:103310-103310(2020).
CC   -!- FUNCTION: Neurotoxin that may modulate ions channels (other than those
CC       tested) (PubMed:31870846). In vivo, induces neurotoxic effects when
CC       injected into insects (tested on L.cuprina and A.domesticus)
CC       (PubMed:31870846). {ECO:0000269|PubMed:31870846}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30400621,
CC       ECO:0000269|PubMed:31870846}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:30400621, ECO:0000305|PubMed:31870846}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000269|PubMed:31870846}.
CC   -!- MASS SPECTROMETRY: Mass=3906.6; Method=MALDI; Note=Monoisotopic mass.;
CC       Evidence={ECO:0000269|PubMed:30400621};
CC   -!- MASS SPECTROMETRY: Mass=3906.80; Method=MALDI; Note=Monoisotopic mass.;
CC       Evidence={ECO:0000269|PubMed:31870846};
CC   -!- MISCELLANEOUS: The recombinant peptide has no effect on Kv1.3/KCNA3,
CC       Kv10.1/KCNH1/EAG1, Kv11.1/KCNH2/ERG1, KCa1.1/KCNMA1, and on the
CC       drosophila Shaker IR channel. {ECO:0000269|PubMed:31870846}.
CC   -!- SIMILARITY: Belongs to the asilidin-1 family. {ECO:0000305}.
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DR   EMBL; MK075130; AYV99533.1; -; mRNA.
DR   PDB; 6PX7; NMR; -; A=47-80.
DR   PDBsum; 6PX7; -.
DR   AlphaFoldDB; A0A3G5BIC2; -.
DR   BMRB; A0A3G5BIC2; -.
DR   SMR; A0A3G5BIC2; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   3D-structure; Disulfide bond; Ion channel impairing toxin; Knottin;
KW   Neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   PROPEP          25..46
FT                   /evidence="ECO:0000305|PubMed:30400621"
FT                   /id="PRO_0000452538"
FT   CHAIN           47..80
FT                   /note="U-Asilidin(1)-Dg12"
FT                   /evidence="ECO:0000269|PubMed:30400621"
FT                   /id="PRO_5018286706"
FT   DISULFID        53..67
FT                   /evidence="ECO:0000269|PubMed:31870846,
FT                   ECO:0007744|PDB:6PX7"
FT   DISULFID        60..71
FT                   /evidence="ECO:0000269|PubMed:31870846,
FT                   ECO:0007744|PDB:6PX7"
FT   DISULFID        66..78
FT                   /evidence="ECO:0000269|PubMed:31870846,
FT                   ECO:0007744|PDB:6PX7"
FT   TURN            63..65
FT                   /evidence="ECO:0007829|PDB:6PX7"
FT   TURN            73..75
FT                   /evidence="ECO:0007829|PDB:6PX7"
SQ   SEQUENCE   80 AA;  8756 MW;  2348295A9E1DA9FD CRC64;
     MARLLVVSVG VFLAVIMLSS ETMSLPAGEN LPALTLFEAQ NQLIGLSQEQ RQCKKIGEHC
     YVADECCSKR CLFYAAKCVS