ID   AS48L_BACIA             Reviewed;         108 AA.
AC   P0DTW2;
DT   12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT   12-AUG-2020, sequence version 1.
DT   25-MAY-2022, entry version 5.
DE   RecName: Full=Pumilarin {ECO:0000303|PubMed:29177092};
DE   AltName: Full=Circular bacteriocin {ECO:0000303|PubMed:29177092};
DE   AltName: Full=Enterocin AS-48-like {ECO:0000303|PubMed:29177092};
DE   AltName: Full=Head-to-tail cyclized peptide {ECO:0000303|PubMed:29177092};
DE   Flags: Precursor;
GN   ORFNames=B4107_2383;
OS   Bacillus safensis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=561879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B4107;
RX   PubMed=25767247; DOI=10.1128/genomea.01539-14;
RA   de Jong A., van Heel A.J., Montalban-Lopez M., Krawczyk A.O.,
RA   Berendsen E.M., Wells-Bennik M., Kuipers O.P.;
RT   "Draft genome sequences of five spore-forming food isolates of Bacillus
RT   pumilus.";
RL   Genome Announc. 3:0-0(2015).
RN   [2]
RP   FUNCTION, MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND CROSS-LINK.
RC   STRAIN=B4107;
RX   PubMed=29177092; DOI=10.1099/mgen.0.000134;
RA   van Heel A.J., Montalban-Lopez M., Oliveau Q., Kuipers O.P.;
RT   "Genome-guided identification of novel head-to-tail cyclized antimicrobial
RT   peptides, exemplified by the discovery of pumilarin.";
RL   Microb. Genom. 3:e000134-e000134(2017).
CC   -!- FUNCTION: Cyclopeptide antibiotic that inhibits both Gram-positive and
CC       Gram-negative bacteria (PubMed:29177092). Shows potent to weak
CC       activities against M.flavus (MIC=3 ug/ml), B.cereus (MIC=12 ug/ml),
CC       B.pumilus (MIC=12 ug/ml), E.coli (MIC=12 ug/ml), and S.pneumoniae
CC       (MIC=47 ug/ml) (PubMed:29177092). May act by forming pores (Probable).
CC       {ECO:0000269|PubMed:29177092, ECO:0000305|PubMed:29177092}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:29177092}.
CC   -!- PTM: The cross-link permits a high resistance to proteolysis. Is more
CC       resistant to specific proteases than to unspecific proteases.
CC       {ECO:0000269|PubMed:29177092}.
CC   -!- MASS SPECTROMETRY: Mass=7083.07; Method=MALDI; Note=Monoisotopic mass.;
CC       Evidence={ECO:0000269|PubMed:29177092};
CC   -!- CAUTION: Strain B4107 was originally classified as being from Bacillus
CC       pumilus, hence protein name pumilarin. {ECO:0000305}.
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DR   EMBL; JXCK01000019; KIL16204.1; -; Genomic_DNA.
DR   RefSeq; WP_024423066.1; NZ_WCHQ01000001.1.
DR   AlphaFoldDB; P0DTW2; -.
DR   SMR; P0DTW2; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.225.10; -; 1.
DR   InterPro; IPR009086; Bacteriocin_AS48.
DR   InterPro; IPR020038; Circ_bacteriocin.
DR   Pfam; PF09221; Bacteriocin_IId; 1.
DR   TIGRFAMs; TIGR03651; circ_ocin_uber; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Secreted.
FT   PROPEP          1..38
FT                   /evidence="ECO:0000305|PubMed:29177092"
FT                   /id="PRO_0000450463"
FT   CHAIN           39..108
FT                   /note="Pumilarin"
FT                   /evidence="ECO:0000269|PubMed:29177092"
FT                   /id="PRO_0000450464"
FT   CROSSLNK        39..108
FT                   /note="Cyclopeptide (Leu-Trp)"
FT                   /evidence="ECO:0000269|PubMed:29177092"
SQ   SEQUENCE   108 AA;  11284 MW;  9044ABA177AA5327 CRC64;
     MTETKNEIKL HVLFGALAVG FLMLALFSFS LQMLPVADLA KEFGIPGSVA AVVLNVVEAG
     GAVTTIVSIL TAVGSGGLSL IAAAGKETIR QYLKNEIKKK GRKAVIAW