OPSG_MOUSE
ID OPSG_MOUSE Reviewed; 359 AA.
AC O35599; Q548Z4;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Medium-wave-sensitive opsin 1;
DE AltName: Full=Green cone photoreceptor pigment;
DE AltName: Full=Green-sensitive opsin;
DE Short=M opsin;
DE AltName: Full=Medium wavelength-sensitive cone opsin;
GN Name=Opn1mw; Synonyms=Gcp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9238068; DOI=10.1073/pnas.94.16.8860;
RA Sun H., Macke J.P., Nathans J.;
RT "Mechanisms of spectral tuning in the mouse green cone pigment.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:8860-8865(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=BALB/cJ, and C57BL/10;
RX PubMed=11055434; DOI=10.1016/s0896-6273(00)00062-3;
RA Applebury M.L., Antoch M.P., Baxter L.C., Chun L.Y., Falk J.D.,
RA Farhangfar F., Kage K., Krzystolik M.G., Lyass L.A., Robbins J.T.;
RT "The murine cone photoreceptor: a single cone type expresses both S and M
RT opsins with retinal spatial patterning.";
RL Neuron 27:513-523(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Retina;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP DEVELOPMENTAL STAGE.
RX PubMed=23001562; DOI=10.1093/hmg/dds398;
RA Alves C.H., Sanz A.S., Park B., Pellissier L.P., Tanimoto N., Beck S.C.,
RA Huber G., Murtaza M., Richard F., Sridevi Gurubaran I., Garcia Garrido M.,
RA Levelt C.N., Rashbass P., Le Bivic A., Seeliger M.W., Wijnholds J.;
RT "Loss of CRB2 in the mouse retina mimics human retinitis pigmentosa due to
RT mutations in the CRB1 gene.";
RL Hum. Mol. Genet. 22:35-50(2013).
RN [5]
RP TISSUE SPECIFICITY, AND GLYCOSYLATION.
RX PubMed=30948514; DOI=10.1074/jbc.ra118.006835;
RA Salom D., Jin H., Gerken T.A., Yu C., Huang L., Palczewski K.;
RT "Human red and green cone opsins are O-glycosylated at an N-terminal
RT Ser/Thr-rich domain conserved in vertebrates.";
RL J. Biol. Chem. 294:8123-8133(2019).
CC -!- FUNCTION: Visual pigments are the light-absorbing molecules that
CC mediate vision. They consist of an apoprotein, opsin, covalently linked
CC to cis-retinal. May increase spectral sensitivity in dim light.
CC {ECO:0000269|PubMed:11055434}.
CC -!- SUBUNIT: Monomer. Homodimer. Homotetramer.
CC {ECO:0000250|UniProtKB:P04001}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P04001};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in retina (at protein level)
CC (PubMed:30948514). Expressed in cone photoreceptor cells (at protein
CC level) (PubMed:11055434). {ECO:0000269|PubMed:11055434,
CC ECO:0000269|PubMed:30948514}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the retinal outer nuclear layer and
CC the inner nuclear layer at postnatal day 10.
CC {ECO:0000269|PubMed:23001562}.
CC -!- PTM: N-glycosylated (PubMed:30948514). O-glycosylated
CC (PubMed:30948514). {ECO:0000269|PubMed:30948514}.
CC -!- PTM: Phosphorylated on some or all of the serine and threonine residues
CC present in the C-terminal region. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Opsin
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AF011389; AAB64302.1; -; mRNA.
DR EMBL; AF190672; AAG17991.1; -; mRNA.
DR EMBL; AF191085; AAG17992.1; -; Genomic_DNA.
DR EMBL; AF191080; AAG17992.1; JOINED; Genomic_DNA.
DR EMBL; AF191081; AAG17992.1; JOINED; Genomic_DNA.
DR EMBL; AF191082; AAG17992.1; JOINED; Genomic_DNA.
DR EMBL; AF191083; AAG17992.1; JOINED; Genomic_DNA.
DR EMBL; AF191084; AAG17992.1; JOINED; Genomic_DNA.
DR EMBL; BC014826; AAH14826.1; -; mRNA.
DR CCDS; CCDS30220.1; -.
DR RefSeq; NP_032132.1; NM_008106.2.
DR AlphaFoldDB; O35599; -.
DR SMR; O35599; -.
DR STRING; 10090.ENSMUSP00000033771; -.
DR GlyGen; O35599; 1 site.
DR PhosphoSitePlus; O35599; -.
DR PaxDb; O35599; -.
DR PRIDE; O35599; -.
DR ProteomicsDB; 294394; -.
DR DNASU; 14539; -.
DR Ensembl; ENSMUST00000033771; ENSMUSP00000033771; ENSMUSG00000031394.
DR GeneID; 14539; -.
DR KEGG; mmu:14539; -.
DR UCSC; uc009tnv.1; mouse.
DR CTD; 2652; -.
DR MGI; MGI:1097692; Opn1mw.
DR VEuPathDB; HostDB:ENSMUSG00000031394; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01030000234549; -.
DR HOGENOM; CLU_009579_3_0_1; -.
DR InParanoid; O35599; -.
DR OMA; EWGKQSF; -.
DR OrthoDB; 940057at2759; -.
DR PhylomeDB; O35599; -.
DR TreeFam; TF324998; -.
DR Reactome; R-MMU-2187335; The retinoid cycle in cones (daylight vision).
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR Reactome; R-MMU-419771; Opsins.
DR BioGRID-ORCS; 14539; 2 hits in 72 CRISPR screens.
DR PRO; PR:O35599; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; O35599; protein.
DR Bgee; ENSMUSG00000031394; Expressed in retinal neural layer and 11 other tissues.
DR ExpressionAtlas; O35599; baseline and differential.
DR Genevisible; O35599; MM.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0001750; C:photoreceptor outer segment; IDA:MGI.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0008020; F:G protein-coupled photoreceptor activity; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; ISS:UniProtKB.
DR GO; GO:0009881; F:photoreceptor activity; ISO:MGI.
DR GO; GO:0071482; P:cellular response to light stimulus; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007602; P:phototransduction; IBA:GO_Central.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR001760; Opsin.
DR InterPro; IPR000378; Opsin_red/grn.
DR InterPro; IPR027430; Retinal_BS.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00238; OPSIN.
DR PRINTS; PR00575; OPSINREDGRN.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR PROSITE; PS00238; OPSIN; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Chromophore; Disulfide bond; G-protein coupled receptor;
KW Glycoprotein; Membrane; Phosphoprotein; Photoreceptor protein; Receptor;
KW Reference proteome; Retinal protein; Sensory transduction; Transducer;
KW Transmembrane; Transmembrane helix; Vision.
FT CHAIN 1..359
FT /note="Medium-wave-sensitive opsin 1"
FT /id="PRO_0000197786"
FT TOPO_DOM 1..47
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..72
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 73..84
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..110
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..124
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..144
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..163
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..187
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..213
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..241
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 242..263
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..287
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 288..295
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..320
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 321..359
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 12..38
FT /note="Required for 11-cis-retinal regeneration"
FT /evidence="ECO:0000250|UniProtKB:P04001"
FT MOD_RES 307
FT /note="N6-(retinylidene)lysine"
FT CARBOHYD 29
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 121..198
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 359 AA; 40218 MW; 1BB5F531A6FAE357 CRC64;
MAQRLTGEQT LDHYEDSTHA SIFTYTNSNS TKGPFEGPNY HIAPRWVYHL TSTWMILVVV
ASVFTNGLVL AATMRFKKLR HPLNWILVNL AVADLAETII ASTISVVNQI YGYFVLGHPL
CVIEGYIVSL CGITGLWSLA IISWERWLVV CKPFGNVRFD AKLATVGIVF SWVWAAIWTA
PPIFGWSRYW PYGLKTSCGP DVFSGTSYPG VQSYMMVLMV TCCIFPLSII VLCYLQVWLA
IRAVAKQQKE SESTQKAEKE VTRMVVVMVF AYCLCWGPYT FFACFATAHP GYAFHPLVAS
LPSYFAKSAT IYNPIIYVFM NRQFRNCILH LFGKKVDDSS ELSSTSKTEV SSVSSVSPA
