OPSG_RAT
ID OPSG_RAT Reviewed; 359 AA.
AC O35476;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Medium-wave-sensitive opsin 1;
DE AltName: Full=Green cone photoreceptor pigment;
DE AltName: Full=Green-sensitive opsin;
DE AltName: Full=Medium wavelength-sensitive cone opsin;
GN Name=Opn1mw; Synonyms=Gcp;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9751808; DOI=10.1016/s0378-1119(98)00359-x;
RA Radlwimmer F.B., Yokoyama S.;
RT "Genetic analyses of the green visual pigments of rabbit (Oryctolagus
RT cuniculus) and rat (Rattus norvegicus).";
RL Gene 218:103-109(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 43-315.
RX PubMed=9580985; DOI=10.1093/oxfordjournals.molbev.a025956;
RA Yokoyama S., Radlwimmer F.B.;
RT "The 'five-sites' rule and the evolution of red and green color vision in
RT mammals.";
RL Mol. Biol. Evol. 15:560-567(1998).
CC -!- FUNCTION: Visual pigments are the light-absorbing molecules that
CC mediate vision. They consist of an apoprotein, opsin, covalently linked
CC to cis-retinal. May increase spectral sensitivity in dim light.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Absorption:
CC Abs(max)=509 nm;
CC -!- SUBUNIT: Monomer. Homodimer. Homotetramer.
CC {ECO:0000250|UniProtKB:P04001}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in cone photoreceptor cells.
CC -!- PTM: O-glycosylated. {ECO:0000250|UniProtKB:O35599}.
CC -!- PTM: Phosphorylated on some or all of the serine and threonine residues
CC present in the C-terminal region.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Opsin
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AF054246; AAC64920.1; -; Genomic_DNA.
DR EMBL; AF054241; AAC64920.1; JOINED; Genomic_DNA.
DR EMBL; AF054242; AAC64920.1; JOINED; Genomic_DNA.
DR EMBL; AF054243; AAC64920.1; JOINED; Genomic_DNA.
DR EMBL; AF054244; AAC64920.1; JOINED; Genomic_DNA.
DR EMBL; AF054245; AAC64920.1; JOINED; Genomic_DNA.
DR EMBL; AF031528; AAB86946.1; -; mRNA.
DR RefSeq; NP_446000.1; NM_053548.1.
DR AlphaFoldDB; O35476; -.
DR SMR; O35476; -.
DR STRING; 10116.ENSRNOP00000053164; -.
DR GlyGen; O35476; 1 site.
DR PhosphoSitePlus; O35476; -.
DR PaxDb; O35476; -.
DR Ensembl; ENSRNOT00000083147; ENSRNOP00000072867; ENSRNOG00000051529.
DR GeneID; 89810; -.
DR KEGG; rno:89810; -.
DR CTD; 2652; -.
DR RGD; 620978; Opn1mw.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01030000234549; -.
DR HOGENOM; CLU_009579_3_0_1; -.
DR InParanoid; O35476; -.
DR OMA; EWGKQSF; -.
DR OrthoDB; 940057at2759; -.
DR Reactome; R-RNO-2187335; The retinoid cycle in cones (daylight vision).
DR Reactome; R-RNO-418594; G alpha (i) signalling events.
DR Reactome; R-RNO-419771; Opsins.
DR PRO; PR:O35476; -.
DR Proteomes; UP000002494; Chromosome X.
DR Genevisible; O35476; RN.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0001750; C:photoreceptor outer segment; IDA:RGD.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0008020; F:G protein-coupled photoreceptor activity; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; ISS:UniProtKB.
DR GO; GO:0009881; F:photoreceptor activity; ISO:RGD.
DR GO; GO:0071482; P:cellular response to light stimulus; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007602; P:phototransduction; IBA:GO_Central.
DR GO; GO:0032467; P:positive regulation of cytokinesis; ISO:RGD.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR001760; Opsin.
DR InterPro; IPR000378; Opsin_red/grn.
DR InterPro; IPR027430; Retinal_BS.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00238; OPSIN.
DR PRINTS; PR00575; OPSINREDGRN.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR PROSITE; PS00238; OPSIN; 1.
PE 1: Evidence at protein level;
KW Chromophore; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Phosphoprotein; Photoreceptor protein; Receptor;
KW Reference proteome; Retinal protein; Sensory transduction; Transducer;
KW Transmembrane; Transmembrane helix; Vision.
FT CHAIN 1..359
FT /note="Medium-wave-sensitive opsin 1"
FT /id="PRO_0000197789"
FT TOPO_DOM 1..47
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..72
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 73..84
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..110
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..124
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..144
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..163
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..187
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..213
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..241
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 242..263
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..287
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 288..295
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..320
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 321..359
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 12..38
FT /note="Required for 11-cis-retinal regeneration"
FT /evidence="ECO:0000250|UniProtKB:P04001"
FT MOD_RES 307
FT /note="N6-(retinylidene)lysine"
FT CARBOHYD 29
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 121..198
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 359 AA; 40200 MW; 08D9294EE6BE8A05 CRC64;
MAQQLTGEQT LDHYEDSTQA SIFTYTNSNS TRGPFEGPNY HIAPRWVYHL TSTWMILVVI
ASVFTNGLVL AATMRFKKLR HPLNWILVNL AVADLAETII ASTISVVNQI YGYFVLGHPL
CVIEGYIVSL CGITGLWSLA IISWERWLVV CKPFGNVRFD AKLATVGIVF SWVWAAVWTA
PPIFGWSRYW PYGLKTSCGP DVFSGTSYPG VQSYMMVLMV TCCIFPLSII VLCYLQVWLA
IRAVAKQQKE SESTQKAEKE VTRMVVVMVF AYCLCWGPYT FFACFATAHP GYAFHPLVAS
LPSYFAKSAT IYNPIIYVFM NRQFRNCILQ LFGKKVDDSS ELSSTSKTEV SSVSSVSPA
