OPSL1_ARATH
ID OPSL1_ARATH Reviewed; 568 AA.
AC Q9LFB9; Q84W52;
DT 07-NOV-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 121.
DE RecName: Full=Protein OCTOPUS-like {ECO:0000303|PubMed:28652362};
DE Short=AtOPSL1 {ECO:0000303|PubMed:28652362};
GN Name=OPSL1 {ECO:0000303|PubMed:28652362};
GN OrderedLocusNames=At5g01170 {ECO:0000312|Araport:AT5G01170};
GN ORFNames=F7J8.150 {ECO:0000312|EMBL:CAB69845.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=28652362; DOI=10.1073/pnas.1703258114;
RA Breda A.S., Hazak O., Hardtke C.S.;
RT "Phosphosite charge rather than shootward localization determines OCTOPUS
RT activity in root protophloem.";
RL Proc. Natl. Acad. Sci. U.S.A. 114:E5721-E5730(2017).
CC -!- FUNCTION: Potentiates primary root protophloem differentiation.
CC Regulates roots architecture. {ECO:0000269|PubMed:28652362}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9SS80};
CC Peripheral membrane protein {ECO:0000250|UniProtKB:Q9SS80}; Cytoplasmic
CC side {ECO:0000250|UniProtKB:Q9SS80}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q9SS80}.
CC -!- PTM: Phosphorylation at Ser-260 amplifies the promotion of protophloem
CC differentiation. {ECO:0000250|UniProtKB:Q9SS80}.
CC -!- MISCELLANEOUS: Can complement the protophloem differentiation defect
CC phenotypes of ops, brx and brx ops mutants.
CC {ECO:0000269|PubMed:28652362}.
CC -!- SIMILARITY: Belongs to the OCTOPUS family. {ECO:0000305}.
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DR EMBL; AL137189; CAB69845.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90306.1; -; Genomic_DNA.
DR EMBL; BT004217; AAO42234.1; -; mRNA.
DR EMBL; BT015922; AAU95458.1; -; mRNA.
DR PIR; T45957; T45957.
DR RefSeq; NP_195737.1; NM_120195.3.
DR AlphaFoldDB; Q9LFB9; -.
DR STRING; 3702.AT5G01170.1; -.
DR iPTMnet; Q9LFB9; -.
DR PaxDb; Q9LFB9; -.
DR PRIDE; Q9LFB9; -.
DR ProteomicsDB; 248762; -.
DR EnsemblPlants; AT5G01170.1; AT5G01170.1; AT5G01170.
DR GeneID; 831732; -.
DR Gramene; AT5G01170.1; AT5G01170.1; AT5G01170.
DR KEGG; ath:AT5G01170; -.
DR Araport; AT5G01170; -.
DR TAIR; locus:2150109; AT5G01170.
DR eggNOG; ENOG502QR95; Eukaryota.
DR HOGENOM; CLU_021226_0_0_1; -.
DR InParanoid; Q9LFB9; -.
DR OMA; FMGRSIP; -.
DR OrthoDB; 604708at2759; -.
DR PhylomeDB; Q9LFB9; -.
DR PRO; PR:Q9LFB9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LFB9; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0010088; P:phloem development; IMP:UniProtKB.
DR GO; GO:2000280; P:regulation of root development; IMP:UniProtKB.
DR InterPro; IPR008004; OCTOPUS-like.
DR PANTHER; PTHR31659; PTHR31659; 1.
DR Pfam; PF05340; DUF740; 3.
PE 2: Evidence at transcript level;
KW Cell membrane; Cytoplasm; Developmental protein; Differentiation; Membrane;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..568
FT /note="Protein OCTOPUS-like"
FT /id="PRO_0000445682"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 78..99
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 168..203
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 242..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 360..428
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 446..512
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 526..558
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 78..94
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 168..182
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 183..203
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 373..396
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 397..419
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 482..511
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 260
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9SS80"
FT CONFLICT 435
FT /note="S -> C (in Ref. 3; AAO42234)"
FT /evidence="ECO:0000305"
FT CONFLICT 516
FT /note="M -> V (in Ref. 3; AAO42234)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 568 AA; 62295 MW; 08CEA88B2C043072 CRC64;
MNLSADQAPV TAVDELAPPS QPHRLSTSCD LHPEERFSGF CPSCLCDRLS VLDHNAAPPP
SSSSRKPPSI SAVSLKALFK PSSSGTNNSN GNGRVRPGFF PELRRTKSFS AKNNEGFSGG
FEPQRRSCDV RLRDDERNLP INEAASVDKI EEEARESSVS EIVLEVTEEA EIEEDEENGE
KDPGEIVEEK SSEIGEEEEE LKPMKDYMDL YSQTKKPSVK DFAGSFFSAA SVFSKKLQKW
KQKQKVKKPR NGVGGGRPQS EIGVGRRSSD TDPRFSLDAG RFSVDIGRIS MDDSRYSLDE
PRASWDGHLI GRTTAARVPL PPSMLSVVEN APLNRSDMQI PSSPSIKPIS GDSDPIIIIP
GGSNQTRDYY TGPPSSRRRK SLDRSNSIRK IVTELEDVKS VSNSTTTIDS NSMETAENKG
NQNGDKKSRR WGKWSILGFI YRKGKDDEEE DRYSRSNSAG MVERSLSESW PEMRNGEGGG
PKMRRSNSNV SWRSSGGGSA RNKSSRYSSK DGENGMLRFY LTPMRRSWKT SGGSGGGGGG
GGGGGWEKTA AKANSHGHSI ARRVMRLY
