OPSR2_DANRE
ID OPSR2_DANRE Reviewed; 356 AA.
AC Q8AYN0; Q6DH62;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Red-sensitive opsin-2;
DE AltName: Full=Opsin-1, long-wave-sensitive 2;
DE Short=Opsin LWS-2;
DE AltName: Full=Red cone photoreceptor pigment 2;
GN Name=opn1lw2; Synonyms=lws2; ORFNames=zgc:92632;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND BIOPHYSICOCHEMICAL
RP PROPERTIES.
RC STRAIN=AB; TISSUE=Eye;
RX PubMed=12618404; DOI=10.1093/genetics/163.2.663;
RA Chinen A., Hamaoka T., Yamada Y., Kawamura S.;
RT "Gene duplication and spectral diversification of cone visual pigments of
RT zebrafish.";
RL Genetics 163:663-675(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Visual pigments are the light-absorbing molecules that
CC mediate vision. They consist of an apoprotein, opsin, covalently linked
CC to cis-retinal.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Absorption:
CC Abs(max)=548 nm {ECO:0000269|PubMed:12618404};
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- PTM: Phosphorylated on some or all of the serine and threonine residues
CC present in the C-terminal region. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Opsin
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AB087804; BAC24128.1; -; Genomic_DNA.
DR EMBL; AL844847; CAE30417.1; -; Genomic_DNA.
DR EMBL; BC076120; AAH76120.1; -; mRNA.
DR RefSeq; NP_001002443.1; NM_001002443.2.
DR AlphaFoldDB; Q8AYN0; -.
DR SMR; Q8AYN0; -.
DR STRING; 7955.ENSDARP00000065939; -.
DR PaxDb; Q8AYN0; -.
DR PRIDE; Q8AYN0; -.
DR Ensembl; ENSDART00000065940; ENSDARP00000065939; ENSDARG00000044861.
DR Ensembl; ENSDART00000189991; ENSDARP00000149112; ENSDARG00000115709.
DR GeneID; 436716; -.
DR KEGG; dre:436716; -.
DR CTD; 436716; -.
DR ZFIN; ZDB-GENE-040718-141; opn1lw2.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01030000234549; -.
DR HOGENOM; CLU_009579_3_0_1; -.
DR InParanoid; Q8AYN0; -.
DR OMA; HISRIFC; -.
DR OrthoDB; 940057at2759; -.
DR PhylomeDB; Q8AYN0; -.
DR TreeFam; TF324998; -.
DR Reactome; R-DRE-2187335; The retinoid cycle in cones (daylight vision).
DR Reactome; R-DRE-418594; G alpha (i) signalling events.
DR Reactome; R-DRE-419771; Opsins.
DR PRO; PR:Q8AYN0; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 11.
DR Bgee; ENSDARG00000044861; Expressed in retina and 4 other tissues.
DR ExpressionAtlas; Q8AYN0; baseline.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0001750; C:photoreceptor outer segment; IBA:GO_Central.
DR GO; GO:0008020; F:G protein-coupled photoreceptor activity; IBA:GO_Central.
DR GO; GO:0071482; P:cellular response to light stimulus; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007602; P:phototransduction; IBA:GO_Central.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR001760; Opsin.
DR InterPro; IPR000378; Opsin_red/grn.
DR InterPro; IPR027430; Retinal_BS.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00238; OPSIN.
DR PRINTS; PR00575; OPSINREDGRN.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR PROSITE; PS00238; OPSIN; 1.
PE 1: Evidence at protein level;
KW Chromophore; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Phosphoprotein; Photoreceptor protein; Receptor;
KW Reference proteome; Retinal protein; Sensory transduction; Transducer;
KW Transmembrane; Transmembrane helix; Vision.
FT CHAIN 1..356
FT /note="Red-sensitive opsin-2"
FT /id="PRO_0000197794"
FT TOPO_DOM 1..48
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..73
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 74..85
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..111
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..125
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..145
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 146..164
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..188
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 189..214
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..242
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 243..264
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..288
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 289..296
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..321
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 322..356
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 308
FT /note="N6-(retinylidene)lysine"
FT /evidence="ECO:0000250"
FT CARBOHYD 30
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 122..199
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 7
FT /note="A -> G (in Ref. 3; AAH76120)"
FT /evidence="ECO:0000305"
FT CONFLICT 102
FT /note="A -> S (in Ref. 3; AAH76120)"
FT /evidence="ECO:0000305"
FT CONFLICT 270
FT /note="I -> V (in Ref. 3; AAH76120)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 356 AA; 39518 MW; FCEA5CE2D665ADC3 CRC64;
MAEWANAAFA ARRRGDETTR DNAFSYTNSN NTRDPFEGPN YHIAPRWVYN VATVWMFFVV
VASTFTNGLV LVATAKFKKL RHPLNWILVN LAIADLGETL FASTISVINQ VFGYFILGHP
MCIFEGYTVS VCGIAGLWSL TVISWERWVV VCKPFGNVKF DGKWASAGII FSWVWAAVWC
APPIFGWSRY WPHGLKTSCG PDVFGGNEDP GVQSYMLVLM ITCCILPLAI IILCYIAVFL
AIHAVAQQQK DSESTQKAEK EVSRMVVVMI LAFCLCWGPY TAFACFAAAN PGYAFHPLAA
AMPAYFAKSA TIYNPIIYVF MNRQFRVCIM QLFGKKVDDG SEVSTSKTEV SSVAPA
