OPT2_ARATH
ID OPT2_ARATH Reviewed; 734 AA.
AC O04514; Q1PFX3;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 2.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Oligopeptide transporter 2;
DE Short=AtOPT2;
GN Name=OPT2; OrderedLocusNames=At1g09930; ORFNames=F21M12.32;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP FUNCTION, NOMENCLATURE, AND TISSUE SPECIFICITY.
RX PubMed=11788749; DOI=10.1104/pp.010332;
RA Koh S., Wiles A.M., Sharp J.S., Naider F.R., Becker J.M., Stacey G.;
RT "An oligopeptide transporter gene family in Arabidopsis.";
RL Plant Physiol. 128:21-29(2002).
RN [5]
RP FUNCTION, AND INDUCTION.
RX PubMed=13129917; DOI=10.1074/jbc.m309338200;
RA Wintz H., Fox T., Wu Y.-Y., Feng V., Chen W., Chang H.-S., Zhu T.,
RA Vulpe C.D.;
RT "Expression profiles of Arabidopsis thaliana in mineral deficiencies reveal
RT novel transporters involved in metal homeostasis.";
RL J. Biol. Chem. 278:47644-47653(2003).
CC -!- FUNCTION: Involved in the translocation of tetra- and pentapeptides
CC across the cellular membrane in an energy-dependent manner.
CC {ECO:0000250, ECO:0000269|PubMed:11788749,
CC ECO:0000269|PubMed:13129917}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in flowers, leaves, roots, and stems.
CC {ECO:0000269|PubMed:11788749}.
CC -!- INDUCTION: Highly induced by iron deficiency.
CC {ECO:0000269|PubMed:13129917}.
CC -!- SIMILARITY: Belongs to the oligopeptide OPT transporter (TC 2.A.67.1)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB60748.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC000132; AAB60748.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28515.1; -; Genomic_DNA.
DR EMBL; DQ446240; ABE65610.1; -; mRNA.
DR PIR; F86233; F86233.
DR RefSeq; NP_172464.1; NM_100867.2.
DR AlphaFoldDB; O04514; -.
DR STRING; 3702.AT1G09930.1; -.
DR PaxDb; O04514; -.
DR PRIDE; O04514; -.
DR ProteomicsDB; 248765; -.
DR EnsemblPlants; AT1G09930.1; AT1G09930.1; AT1G09930.
DR GeneID; 837525; -.
DR Gramene; AT1G09930.1; AT1G09930.1; AT1G09930.
DR KEGG; ath:AT1G09930; -.
DR Araport; AT1G09930; -.
DR TAIR; locus:2024372; AT1G09930.
DR eggNOG; KOG2262; Eukaryota.
DR HOGENOM; CLU_004965_1_1_1; -.
DR InParanoid; O04514; -.
DR OMA; ANVFAGW; -.
DR OrthoDB; 190227at2759; -.
DR PhylomeDB; O04514; -.
DR PRO; PR:O04514; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O04514; baseline and differential.
DR Genevisible; O04514; AT.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; ISS:TAIR.
DR GO; GO:0035673; F:oligopeptide transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR004648; Oligpept_transpt.
DR InterPro; IPR004813; OPT.
DR PANTHER; PTHR22601; PTHR22601; 1.
DR Pfam; PF03169; OPT; 1.
DR TIGRFAMs; TIGR00727; ISP4_OPT; 1.
DR TIGRFAMs; TIGR00728; OPT_sfam; 1.
PE 2: Evidence at transcript level;
KW Membrane; Peptide transport; Protein transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..734
FT /note="Oligopeptide transporter 2"
FT /id="PRO_0000213779"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 359..379
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 414..434
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 525..545
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 596..616
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 644..664
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 677..697
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 456
FT /note="T -> I (in Ref. 3; ABE65610)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 734 AA; 82334 MW; 40622990EAF1E60B CRC64;
MAAIELHKPE INADDDDDES PVEQVRLTVS NHDDPSLPVW TFRMWFLGLL SCILLSFLNT
FFGYRTQPLM ITMISVQVVT LPLGKLMARV LPETKYKIGS WEFSFNPGPF NVKEHVLISM
FANAGAGFGS GTAYAVGIVD IIMAFYKRKI SFLASWILVI TTQILGYGWA GIMRKLVVDP
AQMWWPTSVL QVSLFRALHE KDNARMSRGK FFVIAFVCSF AWYIFPAYLF LTLSSISWVC
WAFPKSITAQ QLGSGMSGLG IGAFALDWSV IASYLGSPLV TPFFAIVNVL VGYVLVMYMV
IPISYWGMNV YEANKFPIFS SDLFDKQGQL YNISTIVNNK FELDMENYQQ QGRVYLSTFF
AISYGIGFAA IVSTLTHVAL FNGKGIWQQV RASTKAKMDI HTRLMKKYKD IPGWWFYSLL
AISLVLSLVL CIFMKDEIQM PWWGLLLASF MALTFTVPVS IITATTNQTP GLNIITEYLM
GVLLPGRPIA NVCFKTYGYI SMSQAISFLN DFKLGHYMKI PPRSMFLVQF IGTVIAGTVN
ISVAWYLLTS VENICQKELL PPNSPWTCPS DRVFFDASVI WGLVGPKRIF GRLGNYPALN
WFFLGGLIGP VLVWLLQKAF PTKTWISQIN LPVLLGATAA MPPATSVNFN CWIIVGVIFN
YFVFKYCKKW WQRYNYVLSA ALDAGLAFMG VLLYFSLTMN GISINHWWGA KGENCPLASC
PTAPGVLVDG CPVF
