OPT2_YEAST
ID OPT2_YEAST Reviewed; 877 AA.
AC Q06593; D6W4J4;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Oligopeptide transporter 2;
GN Name=OPT2; OrderedLocusNames=YPR194C; ORFNames=P9677.13;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION.
RX PubMed=9643541; DOI=10.1046/j.1365-2958.1998.00827.x;
RA Lubkowitz M.A., Barnes D., Breslav M., Burchfield A., Naider F.,
RA Becker J.M.;
RT "Schizosaccharomyces pombe isp4 encodes a transporter representing a novel
RT family of oligopeptide transporters.";
RL Mol. Microbiol. 28:729-741(1998).
RN [4]
RP FUNCTION.
RX PubMed=10788431; DOI=10.1074/jbc.275.18.13259;
RA Bourbouloux A., Shahi P., Chakladar A., Delrot S., Bachhawat A.K.;
RT "Hgt1p, a high affinity glutathione transporter from the yeast
RT Saccharomyces cerevisiae.";
RL J. Biol. Chem. 275:13259-13265(2000).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Transports tetra- and pentapeptides. Does not transport
CC glutathione. {ECO:0000269|PubMed:10788431, ECO:0000269|PubMed:9643541}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the oligopeptide OPT transporter family.
CC {ECO:0000305}.
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DR EMBL; U25841; AAB64623.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11610.1; -; Genomic_DNA.
DR PIR; S58824; S58824.
DR RefSeq; NP_015520.1; NM_001184291.1.
DR AlphaFoldDB; Q06593; -.
DR BioGRID; 36366; 107.
DR DIP; DIP-5482N; -.
DR IntAct; Q06593; 2.
DR STRING; 4932.YPR194C; -.
DR TCDB; 2.A.67.1.4; the oligopeptide transporter (opt) family.
DR PaxDb; Q06593; -.
DR PRIDE; Q06593; -.
DR EnsemblFungi; YPR194C_mRNA; YPR194C; YPR194C.
DR GeneID; 856324; -.
DR KEGG; sce:YPR194C; -.
DR SGD; S000006398; OPT2.
DR VEuPathDB; FungiDB:YPR194C; -.
DR eggNOG; KOG2262; Eukaryota.
DR GeneTree; ENSGT00940000176656; -.
DR HOGENOM; CLU_004965_1_0_1; -.
DR InParanoid; Q06593; -.
DR OMA; QIYPAIK; -.
DR BioCyc; YEAST:G3O-34316-MON; -.
DR PRO; PR:Q06593; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q06593; protein.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0000138; C:Golgi trans cisterna; IDA:SGD.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005777; C:peroxisome; IDA:SGD.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0035673; F:oligopeptide transmembrane transporter activity; IDA:SGD.
DR GO; GO:0045454; P:cell redox homeostasis; IMP:SGD.
DR GO; GO:0006857; P:oligopeptide transport; IDA:SGD.
DR GO; GO:0051515; P:positive regulation of monopolar cell growth; IMP:SGD.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0061091; P:regulation of phospholipid translocation; IGI:SGD.
DR GO; GO:0044088; P:regulation of vacuole organization; IGI:SGD.
DR GO; GO:0042144; P:vacuole fusion, non-autophagic; IMP:SGD.
DR InterPro; IPR004648; Oligpept_transpt.
DR InterPro; IPR004813; OPT.
DR PANTHER; PTHR22601; PTHR22601; 1.
DR Pfam; PF03169; OPT; 1.
DR TIGRFAMs; TIGR00727; ISP4_OPT; 1.
DR TIGRFAMs; TIGR00728; OPT_sfam; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Peptide transport; Protein transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..877
FT /note="Oligopeptide transporter 2"
FT /id="PRO_0000262733"
FT TOPO_DOM 1..167
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 189
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 211..240
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 262..272
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 294..334
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 335..355
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 356..374
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 375..395
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 396..404
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 405..425
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 426..480
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 481..501
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 502..553
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 554..574
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 575..582
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 583..603
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 604..614
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 615..635
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 636..671
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 672..692
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 693..730
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 731..751
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 752..766
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 767..789
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 790..811
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 812..832
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 833..877
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CARBOHYD 374
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 860
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 877 AA; 101261 MW; 3524D342E7EE4B50 CRC64;
MSETVKDKVI IDEKVSTKGT VDYAEGAEYS ERLSNHSSDF SQWYTDEQIL HFMKKLGYEN
RTLYDIPEDV AYILKKMPEL TLEDSFKILK DSIIYFKDDE NIPHDQYEEW KRLVDLEDLD
SKEGIDEYDS FDIRAFASAI KFHSPYQEVR AVVDPEDDPT IPVETFRAYF LAIIWSVIGS
GFNEFFSHRV VSISLNTPII QMFLYICGKA WAKTIPCWTI TIRGRKYGIN IDKPWTQKEQ
MFSTLLYAIC QGAFYTHYNI LTQKLFYHSA FSFGYQFLLS LSVQFIGFGF AGILRKFVVY
PARALWPTVM PTIAINKALL GKEKHESGMS RYKFFFLTFF IMFIYNWFPT YIINILNTFN
WMTWIKPSNI NLANITGGVT GLGINPISSF DWNVISFNSP LVYPFWSYLT QYLGCILAAL
IVIAVYYSNY MSCQYLPIFT NSLYTNTGHS FKVTEVLDSD NKLDVKKYQS YSPPYYSAGN
LVSYGAFICA YPLMITWSFI VHSKLLFNAF KDWALNLWAM RKLKSWVTMF KSDYRALDDY
DDPHSNAMKN YKEVPDWWYF AILIGSLVVG IAVVEHYPTN TPVWGLFVCL GFNFVFLIPT
TILQATTGYS FGLNLLIEMV MGYALPGNPI AIMILKAFGY NIDGQADNYV SNLKIAHYCK
IPPMALFRGQ CVIVFIQIFV NLGVLNWQIS NIKDFCTPHQ NAKFTCPDAV TYYNASVVWG
AIGPKRIFNY IYPIFKWCWL IGACIGIFFG VWKRWGKFYP RYFDPMLFVG GMLNMSPPYN
LMYYTSGMIV SYISQYYMKR HHLNLWEKYN YVLSAGFSTG LVLSAIIIFF AVQYKDTAFN
WWGNTVPYAG ADGVGYPLKN ITDTANGYFG YAPGHYP
